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Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1

The hyperthermophilic archaeon Thermococcus onnurineus NA1 has been shown to produce H(2) when using CO, formate, or starch as a growth substrate. This strain can also utilize elemental sulfur as a terminal electron acceptor for heterotrophic growth. To gain insight into sulfur metabolism, the prote...

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Autores principales: Moon, Yoon-Jung, Kwon, Joseph, Yun, Sung-Ho, Lim, Hye Li, Kim, Jonghyun, Kim, Soo Jung, Kang, Sung Gyun, Lee, Jung-Hyun, Kim, Seung Il, Chung, Young-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463584/
https://www.ncbi.nlm.nih.gov/pubmed/25915030
http://dx.doi.org/10.3390/ijms16059167
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author Moon, Yoon-Jung
Kwon, Joseph
Yun, Sung-Ho
Lim, Hye Li
Kim, Jonghyun
Kim, Soo Jung
Kang, Sung Gyun
Lee, Jung-Hyun
Kim, Seung Il
Chung, Young-Ho
author_facet Moon, Yoon-Jung
Kwon, Joseph
Yun, Sung-Ho
Lim, Hye Li
Kim, Jonghyun
Kim, Soo Jung
Kang, Sung Gyun
Lee, Jung-Hyun
Kim, Seung Il
Chung, Young-Ho
author_sort Moon, Yoon-Jung
collection PubMed
description The hyperthermophilic archaeon Thermococcus onnurineus NA1 has been shown to produce H(2) when using CO, formate, or starch as a growth substrate. This strain can also utilize elemental sulfur as a terminal electron acceptor for heterotrophic growth. To gain insight into sulfur metabolism, the proteome of T. onnurineus NA1 cells grown under sulfur culture conditions was quantified and compared with those grown under H(2)-evolving substrate culture conditions. Using label-free nano-UPLC-MS(E)-based comparative proteomic analysis, approximately 38.4% of the total identified proteome (589 proteins) was found to be significantly up-regulated (≥1.5-fold) under sulfur culture conditions. Many of these proteins were functionally associated with carbon fixation, Fe–S cluster biogenesis, ATP synthesis, sulfur reduction, protein glycosylation, protein translocation, and formate oxidation. Based on the abundances of the identified proteins in this and other genomic studies, the pathways associated with reductive sulfur metabolism, H(2)-metabolism, and oxidative stress defense were proposed. The results also revealed markedly lower expression levels of enzymes involved in the sulfur assimilation pathway, as well as cysteine desulfurase, under sulfur culture condition. The present results provide the first global atlas of proteome changes triggered by sulfur, and may facilitate an understanding of how hyperthermophilic archaea adapt to sulfur-rich, extreme environments.
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spelling pubmed-44635842015-06-16 Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1 Moon, Yoon-Jung Kwon, Joseph Yun, Sung-Ho Lim, Hye Li Kim, Jonghyun Kim, Soo Jung Kang, Sung Gyun Lee, Jung-Hyun Kim, Seung Il Chung, Young-Ho Int J Mol Sci Article The hyperthermophilic archaeon Thermococcus onnurineus NA1 has been shown to produce H(2) when using CO, formate, or starch as a growth substrate. This strain can also utilize elemental sulfur as a terminal electron acceptor for heterotrophic growth. To gain insight into sulfur metabolism, the proteome of T. onnurineus NA1 cells grown under sulfur culture conditions was quantified and compared with those grown under H(2)-evolving substrate culture conditions. Using label-free nano-UPLC-MS(E)-based comparative proteomic analysis, approximately 38.4% of the total identified proteome (589 proteins) was found to be significantly up-regulated (≥1.5-fold) under sulfur culture conditions. Many of these proteins were functionally associated with carbon fixation, Fe–S cluster biogenesis, ATP synthesis, sulfur reduction, protein glycosylation, protein translocation, and formate oxidation. Based on the abundances of the identified proteins in this and other genomic studies, the pathways associated with reductive sulfur metabolism, H(2)-metabolism, and oxidative stress defense were proposed. The results also revealed markedly lower expression levels of enzymes involved in the sulfur assimilation pathway, as well as cysteine desulfurase, under sulfur culture condition. The present results provide the first global atlas of proteome changes triggered by sulfur, and may facilitate an understanding of how hyperthermophilic archaea adapt to sulfur-rich, extreme environments. MDPI 2015-04-23 /pmc/articles/PMC4463584/ /pubmed/25915030 http://dx.doi.org/10.3390/ijms16059167 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moon, Yoon-Jung
Kwon, Joseph
Yun, Sung-Ho
Lim, Hye Li
Kim, Jonghyun
Kim, Soo Jung
Kang, Sung Gyun
Lee, Jung-Hyun
Kim, Seung Il
Chung, Young-Ho
Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1
title Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1
title_full Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1
title_fullStr Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1
title_full_unstemmed Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1
title_short Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1
title_sort proteomic insights into sulfur metabolism in the hydrogen-producing hyperthermophilic archaeon thermococcus onnurineus na1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463584/
https://www.ncbi.nlm.nih.gov/pubmed/25915030
http://dx.doi.org/10.3390/ijms16059167
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