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A Moonlighting Human Protein Is Involved in Mitochondrial Import of tRNA
In yeast Saccharomyces cerevisiae, ~3% of the lysine transfer RNA acceptor 1 (tRK1) pool is imported into mitochondria while the second isoacceptor, tRK2, fully remains in the cytosol. The mitochondrial function of tRK1 is suggested to boost mitochondrial translation under stress conditions. Strikin...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463592/ https://www.ncbi.nlm.nih.gov/pubmed/25918939 http://dx.doi.org/10.3390/ijms16059354 |
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author | Baleva, Maria Gowher, Ali Kamenski, Piotr Tarassov, Ivan Entelis, Nina Masquida, Benoît |
author_facet | Baleva, Maria Gowher, Ali Kamenski, Piotr Tarassov, Ivan Entelis, Nina Masquida, Benoît |
author_sort | Baleva, Maria |
collection | PubMed |
description | In yeast Saccharomyces cerevisiae, ~3% of the lysine transfer RNA acceptor 1 (tRK1) pool is imported into mitochondria while the second isoacceptor, tRK2, fully remains in the cytosol. The mitochondrial function of tRK1 is suggested to boost mitochondrial translation under stress conditions. Strikingly, yeast tRK1 can also be imported into human mitochondria in vivo, and can thus be potentially used as a vector to address RNAs with therapeutic anti-replicative capacity into mitochondria of sick cells. Better understanding of the targeting mechanism in yeast and human is thus critical. Mitochondrial import of tRK1 in yeast proceeds first through a drastic conformational rearrangement of tRK1 induced by enolase 2, which carries this freight to the mitochondrial pre-lysyl-tRNA synthetase (preMSK). The latter may cross the mitochondrial membranes to reach the matrix where imported tRK1 could be used by the mitochondrial translation apparatus. This work focuses on the characterization of the complex that tRK1 forms with human enolases and their role on the interaction between tRK1 and human pre-lysyl-tRNA synthetase (preKARS2). |
format | Online Article Text |
id | pubmed-4463592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-44635922015-06-16 A Moonlighting Human Protein Is Involved in Mitochondrial Import of tRNA Baleva, Maria Gowher, Ali Kamenski, Piotr Tarassov, Ivan Entelis, Nina Masquida, Benoît Int J Mol Sci Article In yeast Saccharomyces cerevisiae, ~3% of the lysine transfer RNA acceptor 1 (tRK1) pool is imported into mitochondria while the second isoacceptor, tRK2, fully remains in the cytosol. The mitochondrial function of tRK1 is suggested to boost mitochondrial translation under stress conditions. Strikingly, yeast tRK1 can also be imported into human mitochondria in vivo, and can thus be potentially used as a vector to address RNAs with therapeutic anti-replicative capacity into mitochondria of sick cells. Better understanding of the targeting mechanism in yeast and human is thus critical. Mitochondrial import of tRK1 in yeast proceeds first through a drastic conformational rearrangement of tRK1 induced by enolase 2, which carries this freight to the mitochondrial pre-lysyl-tRNA synthetase (preMSK). The latter may cross the mitochondrial membranes to reach the matrix where imported tRK1 could be used by the mitochondrial translation apparatus. This work focuses on the characterization of the complex that tRK1 forms with human enolases and their role on the interaction between tRK1 and human pre-lysyl-tRNA synthetase (preKARS2). MDPI 2015-04-24 /pmc/articles/PMC4463592/ /pubmed/25918939 http://dx.doi.org/10.3390/ijms16059354 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Baleva, Maria Gowher, Ali Kamenski, Piotr Tarassov, Ivan Entelis, Nina Masquida, Benoît A Moonlighting Human Protein Is Involved in Mitochondrial Import of tRNA |
title | A Moonlighting Human Protein Is Involved in Mitochondrial Import of tRNA |
title_full | A Moonlighting Human Protein Is Involved in Mitochondrial Import of tRNA |
title_fullStr | A Moonlighting Human Protein Is Involved in Mitochondrial Import of tRNA |
title_full_unstemmed | A Moonlighting Human Protein Is Involved in Mitochondrial Import of tRNA |
title_short | A Moonlighting Human Protein Is Involved in Mitochondrial Import of tRNA |
title_sort | moonlighting human protein is involved in mitochondrial import of trna |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463592/ https://www.ncbi.nlm.nih.gov/pubmed/25918939 http://dx.doi.org/10.3390/ijms16059354 |
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