Intravital FRET: Probing Cellular and Tissue Function in Vivo

The development of intravital Förster Resonance Energy Transfer (FRET) is required to probe cellular and tissue function in the natural context: the living organism. Only in this way can biomedicine truly comprehend pathogenesis and develop effective therapeutic strategies. Here we demonstrate and d...

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Autores principales: Radbruch, Helena, Bremer, Daniel, Mothes, Ronja, Günther, Robert, Rinnenthal, Jan Leo, Pohlan, Julian, Ulbricht, Carolin, Hauser, Anja E., Niesner, Raluca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463726/
https://www.ncbi.nlm.nih.gov/pubmed/26006244
http://dx.doi.org/10.3390/ijms160511713
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author Radbruch, Helena
Bremer, Daniel
Mothes, Ronja
Günther, Robert
Rinnenthal, Jan Leo
Pohlan, Julian
Ulbricht, Carolin
Hauser, Anja E.
Niesner, Raluca
author_facet Radbruch, Helena
Bremer, Daniel
Mothes, Ronja
Günther, Robert
Rinnenthal, Jan Leo
Pohlan, Julian
Ulbricht, Carolin
Hauser, Anja E.
Niesner, Raluca
author_sort Radbruch, Helena
collection PubMed
description The development of intravital Förster Resonance Energy Transfer (FRET) is required to probe cellular and tissue function in the natural context: the living organism. Only in this way can biomedicine truly comprehend pathogenesis and develop effective therapeutic strategies. Here we demonstrate and discuss the advantages and pitfalls of two strategies to quantify FRET in vivo—ratiometrically and time-resolved by fluorescence lifetime imaging—and show their concrete application in the context of neuroinflammation in adult mice.
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spelling pubmed-44637262015-06-16 Intravital FRET: Probing Cellular and Tissue Function in Vivo Radbruch, Helena Bremer, Daniel Mothes, Ronja Günther, Robert Rinnenthal, Jan Leo Pohlan, Julian Ulbricht, Carolin Hauser, Anja E. Niesner, Raluca Int J Mol Sci Article The development of intravital Förster Resonance Energy Transfer (FRET) is required to probe cellular and tissue function in the natural context: the living organism. Only in this way can biomedicine truly comprehend pathogenesis and develop effective therapeutic strategies. Here we demonstrate and discuss the advantages and pitfalls of two strategies to quantify FRET in vivo—ratiometrically and time-resolved by fluorescence lifetime imaging—and show their concrete application in the context of neuroinflammation in adult mice. MDPI 2015-05-21 /pmc/articles/PMC4463726/ /pubmed/26006244 http://dx.doi.org/10.3390/ijms160511713 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Radbruch, Helena
Bremer, Daniel
Mothes, Ronja
Günther, Robert
Rinnenthal, Jan Leo
Pohlan, Julian
Ulbricht, Carolin
Hauser, Anja E.
Niesner, Raluca
Intravital FRET: Probing Cellular and Tissue Function in Vivo
title Intravital FRET: Probing Cellular and Tissue Function in Vivo
title_full Intravital FRET: Probing Cellular and Tissue Function in Vivo
title_fullStr Intravital FRET: Probing Cellular and Tissue Function in Vivo
title_full_unstemmed Intravital FRET: Probing Cellular and Tissue Function in Vivo
title_short Intravital FRET: Probing Cellular and Tissue Function in Vivo
title_sort intravital fret: probing cellular and tissue function in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463726/
https://www.ncbi.nlm.nih.gov/pubmed/26006244
http://dx.doi.org/10.3390/ijms160511713
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