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Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis

Bipolaris papendorfii has been reported as a fungal plant pathogen that rarely causes opportunistic infection in humans. Secondary metabolites isolated from this fungus possess medicinal and anticancer properties. However, its genetic fundamental and basic biology are largely unknown. In this study,...

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Autores principales: Kuan, Chee Sian, Yew, Su Mei, Toh, Yue Fen, Chan, Chai Ling, Ngeow, Yun Fong, Lee, Kok Wei, Na, Shiang Ling, Yee, Wai-Yan, Hoh, Chee-Choong, Ng, Kee Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463846/
https://www.ncbi.nlm.nih.gov/pubmed/25922537
http://dx.doi.org/10.1093/dnares/dsv007
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author Kuan, Chee Sian
Yew, Su Mei
Toh, Yue Fen
Chan, Chai Ling
Ngeow, Yun Fong
Lee, Kok Wei
Na, Shiang Ling
Yee, Wai-Yan
Hoh, Chee-Choong
Ng, Kee Peng
author_facet Kuan, Chee Sian
Yew, Su Mei
Toh, Yue Fen
Chan, Chai Ling
Ngeow, Yun Fong
Lee, Kok Wei
Na, Shiang Ling
Yee, Wai-Yan
Hoh, Chee-Choong
Ng, Kee Peng
author_sort Kuan, Chee Sian
collection PubMed
description Bipolaris papendorfii has been reported as a fungal plant pathogen that rarely causes opportunistic infection in humans. Secondary metabolites isolated from this fungus possess medicinal and anticancer properties. However, its genetic fundamental and basic biology are largely unknown. In this study, we report the first draft genome sequence of B. papendorfii UM 226 isolated from the skin scraping of a patient. The assembled 33.4 Mb genome encodes 11,015 putative coding DNA sequences, of which, 2.49% are predicted transposable elements. Multilocus phylogenetic and phylogenomic analyses showed B. papendorfii UM 226 clustering with Curvularia species, apart from other plant pathogenic Bipolaris species. Its genomic features suggest that it is a heterothallic fungus with a putative unique gene encoding the LysM-containing protein which might be involved in fungal virulence on host plants, as well as a wide array of enzymes involved in carbohydrate metabolism, degradation of polysaccharides and lignin in the plant cell wall, secondary metabolite biosynthesis (including dimethylallyl tryptophan synthase, non-ribosomal peptide synthetase, polyketide synthase), the terpenoid pathway and the caffeine metabolism. This first genomic characterization of B. papendorfii provides the basis for further studies on its biology, pathogenicity and medicinal potential.
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spelling pubmed-44638462015-06-17 Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis Kuan, Chee Sian Yew, Su Mei Toh, Yue Fen Chan, Chai Ling Ngeow, Yun Fong Lee, Kok Wei Na, Shiang Ling Yee, Wai-Yan Hoh, Chee-Choong Ng, Kee Peng DNA Res Full Papers Bipolaris papendorfii has been reported as a fungal plant pathogen that rarely causes opportunistic infection in humans. Secondary metabolites isolated from this fungus possess medicinal and anticancer properties. However, its genetic fundamental and basic biology are largely unknown. In this study, we report the first draft genome sequence of B. papendorfii UM 226 isolated from the skin scraping of a patient. The assembled 33.4 Mb genome encodes 11,015 putative coding DNA sequences, of which, 2.49% are predicted transposable elements. Multilocus phylogenetic and phylogenomic analyses showed B. papendorfii UM 226 clustering with Curvularia species, apart from other plant pathogenic Bipolaris species. Its genomic features suggest that it is a heterothallic fungus with a putative unique gene encoding the LysM-containing protein which might be involved in fungal virulence on host plants, as well as a wide array of enzymes involved in carbohydrate metabolism, degradation of polysaccharides and lignin in the plant cell wall, secondary metabolite biosynthesis (including dimethylallyl tryptophan synthase, non-ribosomal peptide synthetase, polyketide synthase), the terpenoid pathway and the caffeine metabolism. This first genomic characterization of B. papendorfii provides the basis for further studies on its biology, pathogenicity and medicinal potential. Oxford University Press 2015-06 2015-04-27 /pmc/articles/PMC4463846/ /pubmed/25922537 http://dx.doi.org/10.1093/dnares/dsv007 Text en © The Author 2015. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Kuan, Chee Sian
Yew, Su Mei
Toh, Yue Fen
Chan, Chai Ling
Ngeow, Yun Fong
Lee, Kok Wei
Na, Shiang Ling
Yee, Wai-Yan
Hoh, Chee-Choong
Ng, Kee Peng
Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis
title Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis
title_full Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis
title_fullStr Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis
title_full_unstemmed Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis
title_short Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis
title_sort dissecting the fungal biology of bipolaris papendorfii: from phylogenetic to comparative genomic analysis
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463846/
https://www.ncbi.nlm.nih.gov/pubmed/25922537
http://dx.doi.org/10.1093/dnares/dsv007
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