Cargando…
From Glacier to Sauna: RNA-Seq of the Human Pathogen Black Fungus Exophiala dermatitidis under Varying Temperature Conditions Exhibits Common and Novel Fungal Response
Exophiala dermatitidis (Wangiella dermatitidis) belongs to the group of the so-called black yeasts. Thanks in part to its thick and strongly melanized cell walls, E. dermatitidis is extremely tolerant to various kinds of stress, including extreme pH, temperature and desiccation. E. dermatitidis is a...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463862/ https://www.ncbi.nlm.nih.gov/pubmed/26061625 http://dx.doi.org/10.1371/journal.pone.0127103 |
_version_ | 1782375852774785024 |
---|---|
author | Blasi, Barbara Tafer, Hakim Tesei, Donatella Sterflinger, Katja |
author_facet | Blasi, Barbara Tafer, Hakim Tesei, Donatella Sterflinger, Katja |
author_sort | Blasi, Barbara |
collection | PubMed |
description | Exophiala dermatitidis (Wangiella dermatitidis) belongs to the group of the so-called black yeasts. Thanks in part to its thick and strongly melanized cell walls, E. dermatitidis is extremely tolerant to various kinds of stress, including extreme pH, temperature and desiccation. E. dermatitidis is also the agent responsible for various severe illnesses in humans, such as pneumonia and keratitis, and might lead to fatal brain infections. Due to its association with the human environment, its poly-extremophilic lifestyle and its pathogenicity in humans, E. dermatitidis has become an important model organism. In this study we present the functional analysis of the transcriptional response of the fungus at 1°C and 45°C, in comparison with that at 37°C, for two different exposition times, i.e. 1 hour and 1 week. At 1°C, E. dermatitidis uses a large repertoire of tools to acclimatize, such as lipid membrane fluidization, trehalose production or cytoskeleton rearrangement, which allows the fungus to remain metabolically active. At 45°C, the fungus drifts into a replicative state and increases the activity of the Golgi apparatus. As a novel finding, our study provides evidence that, apart from the protein coding genes, non-coding RNAs, circular RNAs as well as fusion-transcripts are differentially regulated and that the function of the fusion-transcripts can be related to the corresponding temperature condition. This work establishes that E. dermatitidis adapts to its environment by modulating coding and non-coding gene transcription levels and through the regulation of chimeric and circular RNAs. |
format | Online Article Text |
id | pubmed-4463862 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44638622015-06-25 From Glacier to Sauna: RNA-Seq of the Human Pathogen Black Fungus Exophiala dermatitidis under Varying Temperature Conditions Exhibits Common and Novel Fungal Response Blasi, Barbara Tafer, Hakim Tesei, Donatella Sterflinger, Katja PLoS One Research Article Exophiala dermatitidis (Wangiella dermatitidis) belongs to the group of the so-called black yeasts. Thanks in part to its thick and strongly melanized cell walls, E. dermatitidis is extremely tolerant to various kinds of stress, including extreme pH, temperature and desiccation. E. dermatitidis is also the agent responsible for various severe illnesses in humans, such as pneumonia and keratitis, and might lead to fatal brain infections. Due to its association with the human environment, its poly-extremophilic lifestyle and its pathogenicity in humans, E. dermatitidis has become an important model organism. In this study we present the functional analysis of the transcriptional response of the fungus at 1°C and 45°C, in comparison with that at 37°C, for two different exposition times, i.e. 1 hour and 1 week. At 1°C, E. dermatitidis uses a large repertoire of tools to acclimatize, such as lipid membrane fluidization, trehalose production or cytoskeleton rearrangement, which allows the fungus to remain metabolically active. At 45°C, the fungus drifts into a replicative state and increases the activity of the Golgi apparatus. As a novel finding, our study provides evidence that, apart from the protein coding genes, non-coding RNAs, circular RNAs as well as fusion-transcripts are differentially regulated and that the function of the fusion-transcripts can be related to the corresponding temperature condition. This work establishes that E. dermatitidis adapts to its environment by modulating coding and non-coding gene transcription levels and through the regulation of chimeric and circular RNAs. Public Library of Science 2015-06-10 /pmc/articles/PMC4463862/ /pubmed/26061625 http://dx.doi.org/10.1371/journal.pone.0127103 Text en © 2015 Blasi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Blasi, Barbara Tafer, Hakim Tesei, Donatella Sterflinger, Katja From Glacier to Sauna: RNA-Seq of the Human Pathogen Black Fungus Exophiala dermatitidis under Varying Temperature Conditions Exhibits Common and Novel Fungal Response |
title | From Glacier to Sauna: RNA-Seq of the Human Pathogen Black Fungus Exophiala dermatitidis under Varying Temperature Conditions Exhibits Common and Novel Fungal Response |
title_full | From Glacier to Sauna: RNA-Seq of the Human Pathogen Black Fungus Exophiala dermatitidis under Varying Temperature Conditions Exhibits Common and Novel Fungal Response |
title_fullStr | From Glacier to Sauna: RNA-Seq of the Human Pathogen Black Fungus Exophiala dermatitidis under Varying Temperature Conditions Exhibits Common and Novel Fungal Response |
title_full_unstemmed | From Glacier to Sauna: RNA-Seq of the Human Pathogen Black Fungus Exophiala dermatitidis under Varying Temperature Conditions Exhibits Common and Novel Fungal Response |
title_short | From Glacier to Sauna: RNA-Seq of the Human Pathogen Black Fungus Exophiala dermatitidis under Varying Temperature Conditions Exhibits Common and Novel Fungal Response |
title_sort | from glacier to sauna: rna-seq of the human pathogen black fungus exophiala dermatitidis under varying temperature conditions exhibits common and novel fungal response |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463862/ https://www.ncbi.nlm.nih.gov/pubmed/26061625 http://dx.doi.org/10.1371/journal.pone.0127103 |
work_keys_str_mv | AT blasibarbara fromglaciertosaunarnaseqofthehumanpathogenblackfungusexophialadermatitidisundervaryingtemperatureconditionsexhibitscommonandnovelfungalresponse AT taferhakim fromglaciertosaunarnaseqofthehumanpathogenblackfungusexophialadermatitidisundervaryingtemperatureconditionsexhibitscommonandnovelfungalresponse AT teseidonatella fromglaciertosaunarnaseqofthehumanpathogenblackfungusexophialadermatitidisundervaryingtemperatureconditionsexhibitscommonandnovelfungalresponse AT sterflingerkatja fromglaciertosaunarnaseqofthehumanpathogenblackfungusexophialadermatitidisundervaryingtemperatureconditionsexhibitscommonandnovelfungalresponse |