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Early embryo achievement through isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. ‘Monreal Rosso’ and ‘Nules’

Microspore embryogenesis is a method of achieving complete homozygosity from plants. It is particularly useful for woody species, like Citrus, characterized by long juvenility, a high degree of heterozygosity and often self-incompatibility. Anther culture is currently the method of choice for micros...

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Autores principales: Chiancone, Benedetta, Karasawa, Marines M. Gniech, Gianguzzi, Valeria, Abdelgalel, Ahmed M., Bárány, Ivett, Testillano, Pilar S., Marinoni, Daniela Torello, Botta, Roberto, Germanà, Maria Antonietta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463929/
https://www.ncbi.nlm.nih.gov/pubmed/26124764
http://dx.doi.org/10.3389/fpls.2015.00413
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author Chiancone, Benedetta
Karasawa, Marines M. Gniech
Gianguzzi, Valeria
Abdelgalel, Ahmed M.
Bárány, Ivett
Testillano, Pilar S.
Marinoni, Daniela Torello
Botta, Roberto
Germanà, Maria Antonietta
author_facet Chiancone, Benedetta
Karasawa, Marines M. Gniech
Gianguzzi, Valeria
Abdelgalel, Ahmed M.
Bárány, Ivett
Testillano, Pilar S.
Marinoni, Daniela Torello
Botta, Roberto
Germanà, Maria Antonietta
author_sort Chiancone, Benedetta
collection PubMed
description Microspore embryogenesis is a method of achieving complete homozygosity from plants. It is particularly useful for woody species, like Citrus, characterized by long juvenility, a high degree of heterozygosity and often self-incompatibility. Anther culture is currently the method of choice for microspore embryogenesis in many crops. However, isolated microspore culture is a better way to investigate the processes at the cellular, physiological, biochemical, and molecular levels as it avoids the influence of somatic anther tissue. To exploit the potential of this technique, it is important to separate the key factors affecting the process and, among them, culture medium composition and particularly the plant growth regulators and their concentration, as they can greatly enhance regeneration efficiency. To our knowledge, the ability of meta-Topolin, a naturally occurring aromatic cytokinin, to induce gametic embryogenesis in isolated microspores of Citrus has never been investigated. In this study, the effect of two concentrations of meta-Topolin instead of benzyladenine or zeatin in the culture medium was investigated in isolated microspore culture of two genotypes of Citrus. After 11 months of isolated microspore culture, for both genotypes and for all the four tested media, the microspore reprogramming and their sporophytic development was observed by the presence of multinucleated calli and microspore-derived embryos at different stages. Microsatellite analysis of parental and embryo samples was performed to determine the embryo alleles constitution of early embryos produced in all tested media, confirming their origin from microspores. To our knowledge, this is the first successful report of Citrus microspore embryogenesis with isolated microspore culture in Citrus, and in particular in Citrus clementina Hort. ex Tan, cvs. ‘Monreal Rosso’ and ‘Nules.’
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spelling pubmed-44639292015-06-29 Early embryo achievement through isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. ‘Monreal Rosso’ and ‘Nules’ Chiancone, Benedetta Karasawa, Marines M. Gniech Gianguzzi, Valeria Abdelgalel, Ahmed M. Bárány, Ivett Testillano, Pilar S. Marinoni, Daniela Torello Botta, Roberto Germanà, Maria Antonietta Front Plant Sci Plant Science Microspore embryogenesis is a method of achieving complete homozygosity from plants. It is particularly useful for woody species, like Citrus, characterized by long juvenility, a high degree of heterozygosity and often self-incompatibility. Anther culture is currently the method of choice for microspore embryogenesis in many crops. However, isolated microspore culture is a better way to investigate the processes at the cellular, physiological, biochemical, and molecular levels as it avoids the influence of somatic anther tissue. To exploit the potential of this technique, it is important to separate the key factors affecting the process and, among them, culture medium composition and particularly the plant growth regulators and their concentration, as they can greatly enhance regeneration efficiency. To our knowledge, the ability of meta-Topolin, a naturally occurring aromatic cytokinin, to induce gametic embryogenesis in isolated microspores of Citrus has never been investigated. In this study, the effect of two concentrations of meta-Topolin instead of benzyladenine or zeatin in the culture medium was investigated in isolated microspore culture of two genotypes of Citrus. After 11 months of isolated microspore culture, for both genotypes and for all the four tested media, the microspore reprogramming and their sporophytic development was observed by the presence of multinucleated calli and microspore-derived embryos at different stages. Microsatellite analysis of parental and embryo samples was performed to determine the embryo alleles constitution of early embryos produced in all tested media, confirming their origin from microspores. To our knowledge, this is the first successful report of Citrus microspore embryogenesis with isolated microspore culture in Citrus, and in particular in Citrus clementina Hort. ex Tan, cvs. ‘Monreal Rosso’ and ‘Nules.’ Frontiers Media S.A. 2015-06-11 /pmc/articles/PMC4463929/ /pubmed/26124764 http://dx.doi.org/10.3389/fpls.2015.00413 Text en Copyright © 2015 Chiancone, Gniech Karasawa, Gianguzzi, Abdelgalel, Bárány, Testillano, Torello Marinoni, Botta and Germanà. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Chiancone, Benedetta
Karasawa, Marines M. Gniech
Gianguzzi, Valeria
Abdelgalel, Ahmed M.
Bárány, Ivett
Testillano, Pilar S.
Marinoni, Daniela Torello
Botta, Roberto
Germanà, Maria Antonietta
Early embryo achievement through isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. ‘Monreal Rosso’ and ‘Nules’
title Early embryo achievement through isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. ‘Monreal Rosso’ and ‘Nules’
title_full Early embryo achievement through isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. ‘Monreal Rosso’ and ‘Nules’
title_fullStr Early embryo achievement through isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. ‘Monreal Rosso’ and ‘Nules’
title_full_unstemmed Early embryo achievement through isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. ‘Monreal Rosso’ and ‘Nules’
title_short Early embryo achievement through isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. ‘Monreal Rosso’ and ‘Nules’
title_sort early embryo achievement through isolated microspore culture in citrus clementina hort. ex tan., cvs. ‘monreal rosso’ and ‘nules’
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463929/
https://www.ncbi.nlm.nih.gov/pubmed/26124764
http://dx.doi.org/10.3389/fpls.2015.00413
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