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Simultaneous bioethanol distillery wastewater treatment and xylanase production by the phyllosphere yeast Pseudozyma antarctica GB-4(0)

Bioethanol production using lignocellulosic biomass generates lignocellulosic bioethanol distillery wastewater (LBDW) that contains a large amount of xylose, making it a potential inexpensive source of xylose for biomaterials production. The main goal of this study was the production of useful enzym...

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Autores principales: Watanabe, Takashi, Suzuki, Ken, Sato, Ikuo, Morita, Tomotake, Koike, Hideaki, Shinozaki, Yukiko, Ueda, Hirokazu, Koitabashi, Motoo, Kitamoto, Hiroko K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463951/
https://www.ncbi.nlm.nih.gov/pubmed/26069206
http://dx.doi.org/10.1186/s13568-015-0121-8
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author Watanabe, Takashi
Suzuki, Ken
Sato, Ikuo
Morita, Tomotake
Koike, Hideaki
Shinozaki, Yukiko
Ueda, Hirokazu
Koitabashi, Motoo
Kitamoto, Hiroko K
author_facet Watanabe, Takashi
Suzuki, Ken
Sato, Ikuo
Morita, Tomotake
Koike, Hideaki
Shinozaki, Yukiko
Ueda, Hirokazu
Koitabashi, Motoo
Kitamoto, Hiroko K
author_sort Watanabe, Takashi
collection PubMed
description Bioethanol production using lignocellulosic biomass generates lignocellulosic bioethanol distillery wastewater (LBDW) that contains a large amount of xylose, making it a potential inexpensive source of xylose for biomaterials production. The main goal of this study was the production of useful enzymes from LBDW during treatment of this wastewater. In this study, we found that xylose strongly induced two yeast strains, Pseudozyma antarctica T-34 and GB-4(0), to produce novel xylanases, PaXynT and PaXynG, respectively. The nucleotide sequence of PaXynT [accession No. DF196774 (GAC73192.1)], obtained from the genome database of strain T-34 using its N-terminal amino acid sequence, was 91% identical to that of PaXynG (accession No. AB901085), and the deduced amino acid sequence is 98% identical. The specific activities of the purified PaXynT and PaXynG were about 52 U/mg. The optimal pH and temperature for both enzymes’ activities were 5.2 and 50°C, respectively. They hydrolyzed xylan to xylose and neither had β-xylosidase (EC 3.2.1.37) activity, indicating that they are endo-β-xylanases (EC 3.2.1.8). With these results, we expect that PaXyns can be employed in saccharizing lignocellulosic biomass materials for the production of useful products just like other endoxylanases. After 72 h of LBDW fed-batch cultivation using a jar-fermentor, strain GB-4(0) produced 17.3 U/ml (corresponding to about 0.3 g/l) of PaXynG and removed 63% of dissolved organic carbon and 87% of dissolved total phosphorus from LBDW. These results demonstrate the potential of P. antarctica for xylanase production during LBDW treatment.
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spelling pubmed-44639512015-06-17 Simultaneous bioethanol distillery wastewater treatment and xylanase production by the phyllosphere yeast Pseudozyma antarctica GB-4(0) Watanabe, Takashi Suzuki, Ken Sato, Ikuo Morita, Tomotake Koike, Hideaki Shinozaki, Yukiko Ueda, Hirokazu Koitabashi, Motoo Kitamoto, Hiroko K AMB Express Original Article Bioethanol production using lignocellulosic biomass generates lignocellulosic bioethanol distillery wastewater (LBDW) that contains a large amount of xylose, making it a potential inexpensive source of xylose for biomaterials production. The main goal of this study was the production of useful enzymes from LBDW during treatment of this wastewater. In this study, we found that xylose strongly induced two yeast strains, Pseudozyma antarctica T-34 and GB-4(0), to produce novel xylanases, PaXynT and PaXynG, respectively. The nucleotide sequence of PaXynT [accession No. DF196774 (GAC73192.1)], obtained from the genome database of strain T-34 using its N-terminal amino acid sequence, was 91% identical to that of PaXynG (accession No. AB901085), and the deduced amino acid sequence is 98% identical. The specific activities of the purified PaXynT and PaXynG were about 52 U/mg. The optimal pH and temperature for both enzymes’ activities were 5.2 and 50°C, respectively. They hydrolyzed xylan to xylose and neither had β-xylosidase (EC 3.2.1.37) activity, indicating that they are endo-β-xylanases (EC 3.2.1.8). With these results, we expect that PaXyns can be employed in saccharizing lignocellulosic biomass materials for the production of useful products just like other endoxylanases. After 72 h of LBDW fed-batch cultivation using a jar-fermentor, strain GB-4(0) produced 17.3 U/ml (corresponding to about 0.3 g/l) of PaXynG and removed 63% of dissolved organic carbon and 87% of dissolved total phosphorus from LBDW. These results demonstrate the potential of P. antarctica for xylanase production during LBDW treatment. Springer Berlin Heidelberg 2015-06-12 /pmc/articles/PMC4463951/ /pubmed/26069206 http://dx.doi.org/10.1186/s13568-015-0121-8 Text en © Watanabe et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Watanabe, Takashi
Suzuki, Ken
Sato, Ikuo
Morita, Tomotake
Koike, Hideaki
Shinozaki, Yukiko
Ueda, Hirokazu
Koitabashi, Motoo
Kitamoto, Hiroko K
Simultaneous bioethanol distillery wastewater treatment and xylanase production by the phyllosphere yeast Pseudozyma antarctica GB-4(0)
title Simultaneous bioethanol distillery wastewater treatment and xylanase production by the phyllosphere yeast Pseudozyma antarctica GB-4(0)
title_full Simultaneous bioethanol distillery wastewater treatment and xylanase production by the phyllosphere yeast Pseudozyma antarctica GB-4(0)
title_fullStr Simultaneous bioethanol distillery wastewater treatment and xylanase production by the phyllosphere yeast Pseudozyma antarctica GB-4(0)
title_full_unstemmed Simultaneous bioethanol distillery wastewater treatment and xylanase production by the phyllosphere yeast Pseudozyma antarctica GB-4(0)
title_short Simultaneous bioethanol distillery wastewater treatment and xylanase production by the phyllosphere yeast Pseudozyma antarctica GB-4(0)
title_sort simultaneous bioethanol distillery wastewater treatment and xylanase production by the phyllosphere yeast pseudozyma antarctica gb-4(0)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463951/
https://www.ncbi.nlm.nih.gov/pubmed/26069206
http://dx.doi.org/10.1186/s13568-015-0121-8
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