Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens

The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various...

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Autores principales: Mediavilla-Gradolph, María Concepción, De Toro-Peinado, Inmaculada, Bermúdez-Ruiz, María Pilar, García-Martínez, María de los Ángeles, Ortega-Torres, María, Montiel Quezel-Guerraz, Natalia, Palop-Borrás, Begoña
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
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Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463989/
https://www.ncbi.nlm.nih.gov/pubmed/26106617
http://dx.doi.org/10.1155/2015/854078
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author Mediavilla-Gradolph, María Concepción
De Toro-Peinado, Inmaculada
Bermúdez-Ruiz, María Pilar
García-Martínez, María de los Ángeles
Ortega-Torres, María
Montiel Quezel-Guerraz, Natalia
Palop-Borrás, Begoña
author_facet Mediavilla-Gradolph, María Concepción
De Toro-Peinado, Inmaculada
Bermúdez-Ruiz, María Pilar
García-Martínez, María de los Ángeles
Ortega-Torres, María
Montiel Quezel-Guerraz, Natalia
Palop-Borrás, Begoña
author_sort Mediavilla-Gradolph, María Concepción
collection PubMed
description The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species.
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spelling pubmed-44639892015-06-23 Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens Mediavilla-Gradolph, María Concepción De Toro-Peinado, Inmaculada Bermúdez-Ruiz, María Pilar García-Martínez, María de los Ángeles Ortega-Torres, María Montiel Quezel-Guerraz, Natalia Palop-Borrás, Begoña Biomed Res Int Research Article The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species. Hindawi Publishing Corporation 2015 2015-05-28 /pmc/articles/PMC4463989/ /pubmed/26106617 http://dx.doi.org/10.1155/2015/854078 Text en Copyright © 2015 María Concepción Mediavilla-Gradolph et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mediavilla-Gradolph, María Concepción
De Toro-Peinado, Inmaculada
Bermúdez-Ruiz, María Pilar
García-Martínez, María de los Ángeles
Ortega-Torres, María
Montiel Quezel-Guerraz, Natalia
Palop-Borrás, Begoña
Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens
title Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens
title_full Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens
title_fullStr Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens
title_full_unstemmed Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens
title_short Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens
title_sort use of maldi-tof ms for identification of nontuberculous mycobacterium species isolated from clinical specimens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463989/
https://www.ncbi.nlm.nih.gov/pubmed/26106617
http://dx.doi.org/10.1155/2015/854078
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