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Mechanical stability of bivalent transition metal complexes analyzed by single-molecule force spectroscopy

Multivalent biomolecular interactions allow for a balanced interplay of mechanical stability and malleability, and nature makes widely use of it. For instance, systems of similar thermal stability may have very different rupture forces. Thus it is of paramount interest to study and understand the me...

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Autores principales: Gensler, Manuel, Eidamshaus, Christian, Taszarek, Maurice, Reissig, Hans-Ulrich, Rabe, Jürgen P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Beilstein-Institut 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464087/
https://www.ncbi.nlm.nih.gov/pubmed/26124883
http://dx.doi.org/10.3762/bjoc.11.91
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author Gensler, Manuel
Eidamshaus, Christian
Taszarek, Maurice
Reissig, Hans-Ulrich
Rabe, Jürgen P
author_facet Gensler, Manuel
Eidamshaus, Christian
Taszarek, Maurice
Reissig, Hans-Ulrich
Rabe, Jürgen P
author_sort Gensler, Manuel
collection PubMed
description Multivalent biomolecular interactions allow for a balanced interplay of mechanical stability and malleability, and nature makes widely use of it. For instance, systems of similar thermal stability may have very different rupture forces. Thus it is of paramount interest to study and understand the mechanical properties of multivalent systems through well-characterized model systems. We analyzed the rupture behavior of three different bivalent pyridine coordination complexes with Cu(2+) in aqueous environment by single-molecule force spectroscopy. Those complexes share the same supramolecular interaction leading to similar thermal off-rates in the range of 0.09 and 0.36 s(−1), compared to 1.7 s(−1) for the monovalent complex. On the other hand, the backbones exhibit different flexibility, and we determined a broad range of rupture lengths between 0.3 and 1.1 nm, with higher most-probable rupture forces for the stiffer backbones. Interestingly, the medium-flexible connection has the highest rupture forces, whereas the ligands with highest and lowest rigidity seem to be prone to consecutive bond rupture. The presented approach allows separating bond and backbone effects in multivalent model systems.
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spelling pubmed-44640872015-06-29 Mechanical stability of bivalent transition metal complexes analyzed by single-molecule force spectroscopy Gensler, Manuel Eidamshaus, Christian Taszarek, Maurice Reissig, Hans-Ulrich Rabe, Jürgen P Beilstein J Org Chem Full Research Paper Multivalent biomolecular interactions allow for a balanced interplay of mechanical stability and malleability, and nature makes widely use of it. For instance, systems of similar thermal stability may have very different rupture forces. Thus it is of paramount interest to study and understand the mechanical properties of multivalent systems through well-characterized model systems. We analyzed the rupture behavior of three different bivalent pyridine coordination complexes with Cu(2+) in aqueous environment by single-molecule force spectroscopy. Those complexes share the same supramolecular interaction leading to similar thermal off-rates in the range of 0.09 and 0.36 s(−1), compared to 1.7 s(−1) for the monovalent complex. On the other hand, the backbones exhibit different flexibility, and we determined a broad range of rupture lengths between 0.3 and 1.1 nm, with higher most-probable rupture forces for the stiffer backbones. Interestingly, the medium-flexible connection has the highest rupture forces, whereas the ligands with highest and lowest rigidity seem to be prone to consecutive bond rupture. The presented approach allows separating bond and backbone effects in multivalent model systems. Beilstein-Institut 2015-05-15 /pmc/articles/PMC4464087/ /pubmed/26124883 http://dx.doi.org/10.3762/bjoc.11.91 Text en Copyright © 2015, Gensler et al. https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjoc/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (https://www.beilstein-journals.org/bjoc/terms)
spellingShingle Full Research Paper
Gensler, Manuel
Eidamshaus, Christian
Taszarek, Maurice
Reissig, Hans-Ulrich
Rabe, Jürgen P
Mechanical stability of bivalent transition metal complexes analyzed by single-molecule force spectroscopy
title Mechanical stability of bivalent transition metal complexes analyzed by single-molecule force spectroscopy
title_full Mechanical stability of bivalent transition metal complexes analyzed by single-molecule force spectroscopy
title_fullStr Mechanical stability of bivalent transition metal complexes analyzed by single-molecule force spectroscopy
title_full_unstemmed Mechanical stability of bivalent transition metal complexes analyzed by single-molecule force spectroscopy
title_short Mechanical stability of bivalent transition metal complexes analyzed by single-molecule force spectroscopy
title_sort mechanical stability of bivalent transition metal complexes analyzed by single-molecule force spectroscopy
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464087/
https://www.ncbi.nlm.nih.gov/pubmed/26124883
http://dx.doi.org/10.3762/bjoc.11.91
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