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Dietary ω3 fatty acid exerts anti-allergic effect through the conversion to 17,18-epoxyeicosatetraenoic acid in the gut

ω3 polyunsaturated fatty acids (PUFAs) have anti-allergic and anti-inflammatory properties, but the immune-metabolic progression from dietary oil remains to be investigated. Here we identified 17,18-epoxyeicostetraenoic acid (17,18-EpETE) as an anti-allergic metabolite generated in the gut from diet...

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Autores principales: Kunisawa, Jun, Arita, Makoto, Hayasaka, Takahiro, Harada, Takashi, Iwamoto, Ryo, Nagasawa, Risa, Shikata, Shiori, Nagatake, Takahiro, Suzuki, Hidehiko, Hashimoto, Eri, Kurashima, Yosuke, Suzuki, Yuji, Arai, Hiroyuki, Setou, Mitsutoshi, Kiyono, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464255/
https://www.ncbi.nlm.nih.gov/pubmed/26065911
http://dx.doi.org/10.1038/srep09750
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author Kunisawa, Jun
Arita, Makoto
Hayasaka, Takahiro
Harada, Takashi
Iwamoto, Ryo
Nagasawa, Risa
Shikata, Shiori
Nagatake, Takahiro
Suzuki, Hidehiko
Hashimoto, Eri
Kurashima, Yosuke
Suzuki, Yuji
Arai, Hiroyuki
Setou, Mitsutoshi
Kiyono, Hiroshi
author_facet Kunisawa, Jun
Arita, Makoto
Hayasaka, Takahiro
Harada, Takashi
Iwamoto, Ryo
Nagasawa, Risa
Shikata, Shiori
Nagatake, Takahiro
Suzuki, Hidehiko
Hashimoto, Eri
Kurashima, Yosuke
Suzuki, Yuji
Arai, Hiroyuki
Setou, Mitsutoshi
Kiyono, Hiroshi
author_sort Kunisawa, Jun
collection PubMed
description ω3 polyunsaturated fatty acids (PUFAs) have anti-allergic and anti-inflammatory properties, but the immune-metabolic progression from dietary oil remains to be investigated. Here we identified 17,18-epoxyeicostetraenoic acid (17,18-EpETE) as an anti-allergic metabolite generated in the gut from dietary ω3 α-linolenic acid (ALA). Biochemical and imaging mass spectrometry analyses revealed increased ALA and its metabolites, especially eicosapentaenoic acid (EPA), in the intestines of mice receiving ALA-rich linseed oil (Lin-mice). In murine food allergy model, the decreased incidence of allergic diarrhea in Lin-mice was due to impairment of mast cell degranulation without affecting allergen-specific serum IgE. Liquid chromatography–tandem mass spectrometry-based mediator lipidomics identified 17,18-EpETE as a major ω3 EPA-derived metabolite generated from dietary ALA in the gut, and 17,18-EpETE exhibits anti-allergic function when administered in vivo. These findings suggest that metabolizing dietary ω3 PUFAs generates 17,18-EpETE, which is an endogenous anti-allergic metabolite and potentially is a therapeutic target to control intestinal allergies.
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spelling pubmed-44642552015-06-18 Dietary ω3 fatty acid exerts anti-allergic effect through the conversion to 17,18-epoxyeicosatetraenoic acid in the gut Kunisawa, Jun Arita, Makoto Hayasaka, Takahiro Harada, Takashi Iwamoto, Ryo Nagasawa, Risa Shikata, Shiori Nagatake, Takahiro Suzuki, Hidehiko Hashimoto, Eri Kurashima, Yosuke Suzuki, Yuji Arai, Hiroyuki Setou, Mitsutoshi Kiyono, Hiroshi Sci Rep Article ω3 polyunsaturated fatty acids (PUFAs) have anti-allergic and anti-inflammatory properties, but the immune-metabolic progression from dietary oil remains to be investigated. Here we identified 17,18-epoxyeicostetraenoic acid (17,18-EpETE) as an anti-allergic metabolite generated in the gut from dietary ω3 α-linolenic acid (ALA). Biochemical and imaging mass spectrometry analyses revealed increased ALA and its metabolites, especially eicosapentaenoic acid (EPA), in the intestines of mice receiving ALA-rich linseed oil (Lin-mice). In murine food allergy model, the decreased incidence of allergic diarrhea in Lin-mice was due to impairment of mast cell degranulation without affecting allergen-specific serum IgE. Liquid chromatography–tandem mass spectrometry-based mediator lipidomics identified 17,18-EpETE as a major ω3 EPA-derived metabolite generated from dietary ALA in the gut, and 17,18-EpETE exhibits anti-allergic function when administered in vivo. These findings suggest that metabolizing dietary ω3 PUFAs generates 17,18-EpETE, which is an endogenous anti-allergic metabolite and potentially is a therapeutic target to control intestinal allergies. Nature Publishing Group 2015-06-11 /pmc/articles/PMC4464255/ /pubmed/26065911 http://dx.doi.org/10.1038/srep09750 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kunisawa, Jun
Arita, Makoto
Hayasaka, Takahiro
Harada, Takashi
Iwamoto, Ryo
Nagasawa, Risa
Shikata, Shiori
Nagatake, Takahiro
Suzuki, Hidehiko
Hashimoto, Eri
Kurashima, Yosuke
Suzuki, Yuji
Arai, Hiroyuki
Setou, Mitsutoshi
Kiyono, Hiroshi
Dietary ω3 fatty acid exerts anti-allergic effect through the conversion to 17,18-epoxyeicosatetraenoic acid in the gut
title Dietary ω3 fatty acid exerts anti-allergic effect through the conversion to 17,18-epoxyeicosatetraenoic acid in the gut
title_full Dietary ω3 fatty acid exerts anti-allergic effect through the conversion to 17,18-epoxyeicosatetraenoic acid in the gut
title_fullStr Dietary ω3 fatty acid exerts anti-allergic effect through the conversion to 17,18-epoxyeicosatetraenoic acid in the gut
title_full_unstemmed Dietary ω3 fatty acid exerts anti-allergic effect through the conversion to 17,18-epoxyeicosatetraenoic acid in the gut
title_short Dietary ω3 fatty acid exerts anti-allergic effect through the conversion to 17,18-epoxyeicosatetraenoic acid in the gut
title_sort dietary ω3 fatty acid exerts anti-allergic effect through the conversion to 17,18-epoxyeicosatetraenoic acid in the gut
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464255/
https://www.ncbi.nlm.nih.gov/pubmed/26065911
http://dx.doi.org/10.1038/srep09750
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