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Comprehensive analysis of Panax ginseng root transcriptomes
BACKGROUND: Korean ginseng (Panax ginseng C.A. Meyer) is a highly effective medicinal plant containing ginsenosides with various pharmacological activities, whose roots are produced commercially for crude drugs. RESULTS: Here, we used the Illumina platform to generate over 232 million RNA sequencing...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464628/ https://www.ncbi.nlm.nih.gov/pubmed/26063328 http://dx.doi.org/10.1186/s12870-015-0527-0 |
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author | Jayakodi, Murukarthick Lee, Sang-Choon Lee, Yun Sun Park, Hyun-Seung Kim, Nam-Hoon Jang, Woojong Lee, Hyun Oh Joh, Ho Jun Yang, Tae-Jin |
author_facet | Jayakodi, Murukarthick Lee, Sang-Choon Lee, Yun Sun Park, Hyun-Seung Kim, Nam-Hoon Jang, Woojong Lee, Hyun Oh Joh, Ho Jun Yang, Tae-Jin |
author_sort | Jayakodi, Murukarthick |
collection | PubMed |
description | BACKGROUND: Korean ginseng (Panax ginseng C.A. Meyer) is a highly effective medicinal plant containing ginsenosides with various pharmacological activities, whose roots are produced commercially for crude drugs. RESULTS: Here, we used the Illumina platform to generate over 232 million RNA sequencing reads from four root samples, including whole roots from one-year-old plants and three types of root tissue from six-year-old plants (i.e., main root bodies, rhizomes, and lateral roots). Through de novo assembly and reference-assisted selection, we obtained a non-redundant unigene set consisting of 55,949 transcripts with an average length of 1,250 bp. Among transcripts in the unigene set, 94 % were functionally annotated via similarity searches against protein databases. Approximately 28.6 % of the transcripts represent novel gene sequences that have not previously been reported for P. ginseng. Digital expression profiling revealed 364 genes showing differential expression patterns among the four root samples. Additionally, 32 were uniquely expressed in one-year-old roots, while seven were uniquely expressed in six-year-old root tissues. We identified 38 transcripts encoding enzymes involved in ginsenoside biosynthesis pathways and 189 encoding UDP-glycosyltransferases. CONCLUSION: Our analysis provides new insights into the role of the root transcriptome in development and secondary metabolite biosynthesis in P. ginseng. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0527-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4464628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44646282015-06-14 Comprehensive analysis of Panax ginseng root transcriptomes Jayakodi, Murukarthick Lee, Sang-Choon Lee, Yun Sun Park, Hyun-Seung Kim, Nam-Hoon Jang, Woojong Lee, Hyun Oh Joh, Ho Jun Yang, Tae-Jin BMC Plant Biol Research Article BACKGROUND: Korean ginseng (Panax ginseng C.A. Meyer) is a highly effective medicinal plant containing ginsenosides with various pharmacological activities, whose roots are produced commercially for crude drugs. RESULTS: Here, we used the Illumina platform to generate over 232 million RNA sequencing reads from four root samples, including whole roots from one-year-old plants and three types of root tissue from six-year-old plants (i.e., main root bodies, rhizomes, and lateral roots). Through de novo assembly and reference-assisted selection, we obtained a non-redundant unigene set consisting of 55,949 transcripts with an average length of 1,250 bp. Among transcripts in the unigene set, 94 % were functionally annotated via similarity searches against protein databases. Approximately 28.6 % of the transcripts represent novel gene sequences that have not previously been reported for P. ginseng. Digital expression profiling revealed 364 genes showing differential expression patterns among the four root samples. Additionally, 32 were uniquely expressed in one-year-old roots, while seven were uniquely expressed in six-year-old root tissues. We identified 38 transcripts encoding enzymes involved in ginsenoside biosynthesis pathways and 189 encoding UDP-glycosyltransferases. CONCLUSION: Our analysis provides new insights into the role of the root transcriptome in development and secondary metabolite biosynthesis in P. ginseng. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0527-0) contains supplementary material, which is available to authorized users. BioMed Central 2015-06-12 /pmc/articles/PMC4464628/ /pubmed/26063328 http://dx.doi.org/10.1186/s12870-015-0527-0 Text en © Jayakodi et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Jayakodi, Murukarthick Lee, Sang-Choon Lee, Yun Sun Park, Hyun-Seung Kim, Nam-Hoon Jang, Woojong Lee, Hyun Oh Joh, Ho Jun Yang, Tae-Jin Comprehensive analysis of Panax ginseng root transcriptomes |
title | Comprehensive analysis of Panax ginseng root transcriptomes |
title_full | Comprehensive analysis of Panax ginseng root transcriptomes |
title_fullStr | Comprehensive analysis of Panax ginseng root transcriptomes |
title_full_unstemmed | Comprehensive analysis of Panax ginseng root transcriptomes |
title_short | Comprehensive analysis of Panax ginseng root transcriptomes |
title_sort | comprehensive analysis of panax ginseng root transcriptomes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464628/ https://www.ncbi.nlm.nih.gov/pubmed/26063328 http://dx.doi.org/10.1186/s12870-015-0527-0 |
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