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Comprehensive analysis of Panax ginseng root transcriptomes

BACKGROUND: Korean ginseng (Panax ginseng C.A. Meyer) is a highly effective medicinal plant containing ginsenosides with various pharmacological activities, whose roots are produced commercially for crude drugs. RESULTS: Here, we used the Illumina platform to generate over 232 million RNA sequencing...

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Autores principales: Jayakodi, Murukarthick, Lee, Sang-Choon, Lee, Yun Sun, Park, Hyun-Seung, Kim, Nam-Hoon, Jang, Woojong, Lee, Hyun Oh, Joh, Ho Jun, Yang, Tae-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464628/
https://www.ncbi.nlm.nih.gov/pubmed/26063328
http://dx.doi.org/10.1186/s12870-015-0527-0
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author Jayakodi, Murukarthick
Lee, Sang-Choon
Lee, Yun Sun
Park, Hyun-Seung
Kim, Nam-Hoon
Jang, Woojong
Lee, Hyun Oh
Joh, Ho Jun
Yang, Tae-Jin
author_facet Jayakodi, Murukarthick
Lee, Sang-Choon
Lee, Yun Sun
Park, Hyun-Seung
Kim, Nam-Hoon
Jang, Woojong
Lee, Hyun Oh
Joh, Ho Jun
Yang, Tae-Jin
author_sort Jayakodi, Murukarthick
collection PubMed
description BACKGROUND: Korean ginseng (Panax ginseng C.A. Meyer) is a highly effective medicinal plant containing ginsenosides with various pharmacological activities, whose roots are produced commercially for crude drugs. RESULTS: Here, we used the Illumina platform to generate over 232 million RNA sequencing reads from four root samples, including whole roots from one-year-old plants and three types of root tissue from six-year-old plants (i.e., main root bodies, rhizomes, and lateral roots). Through de novo assembly and reference-assisted selection, we obtained a non-redundant unigene set consisting of 55,949 transcripts with an average length of 1,250 bp. Among transcripts in the unigene set, 94 % were functionally annotated via similarity searches against protein databases. Approximately 28.6 % of the transcripts represent novel gene sequences that have not previously been reported for P. ginseng. Digital expression profiling revealed 364 genes showing differential expression patterns among the four root samples. Additionally, 32 were uniquely expressed in one-year-old roots, while seven were uniquely expressed in six-year-old root tissues. We identified 38 transcripts encoding enzymes involved in ginsenoside biosynthesis pathways and 189 encoding UDP-glycosyltransferases. CONCLUSION: Our analysis provides new insights into the role of the root transcriptome in development and secondary metabolite biosynthesis in P. ginseng. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0527-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-44646282015-06-14 Comprehensive analysis of Panax ginseng root transcriptomes Jayakodi, Murukarthick Lee, Sang-Choon Lee, Yun Sun Park, Hyun-Seung Kim, Nam-Hoon Jang, Woojong Lee, Hyun Oh Joh, Ho Jun Yang, Tae-Jin BMC Plant Biol Research Article BACKGROUND: Korean ginseng (Panax ginseng C.A. Meyer) is a highly effective medicinal plant containing ginsenosides with various pharmacological activities, whose roots are produced commercially for crude drugs. RESULTS: Here, we used the Illumina platform to generate over 232 million RNA sequencing reads from four root samples, including whole roots from one-year-old plants and three types of root tissue from six-year-old plants (i.e., main root bodies, rhizomes, and lateral roots). Through de novo assembly and reference-assisted selection, we obtained a non-redundant unigene set consisting of 55,949 transcripts with an average length of 1,250 bp. Among transcripts in the unigene set, 94 % were functionally annotated via similarity searches against protein databases. Approximately 28.6 % of the transcripts represent novel gene sequences that have not previously been reported for P. ginseng. Digital expression profiling revealed 364 genes showing differential expression patterns among the four root samples. Additionally, 32 were uniquely expressed in one-year-old roots, while seven were uniquely expressed in six-year-old root tissues. We identified 38 transcripts encoding enzymes involved in ginsenoside biosynthesis pathways and 189 encoding UDP-glycosyltransferases. CONCLUSION: Our analysis provides new insights into the role of the root transcriptome in development and secondary metabolite biosynthesis in P. ginseng. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0527-0) contains supplementary material, which is available to authorized users. BioMed Central 2015-06-12 /pmc/articles/PMC4464628/ /pubmed/26063328 http://dx.doi.org/10.1186/s12870-015-0527-0 Text en © Jayakodi et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Jayakodi, Murukarthick
Lee, Sang-Choon
Lee, Yun Sun
Park, Hyun-Seung
Kim, Nam-Hoon
Jang, Woojong
Lee, Hyun Oh
Joh, Ho Jun
Yang, Tae-Jin
Comprehensive analysis of Panax ginseng root transcriptomes
title Comprehensive analysis of Panax ginseng root transcriptomes
title_full Comprehensive analysis of Panax ginseng root transcriptomes
title_fullStr Comprehensive analysis of Panax ginseng root transcriptomes
title_full_unstemmed Comprehensive analysis of Panax ginseng root transcriptomes
title_short Comprehensive analysis of Panax ginseng root transcriptomes
title_sort comprehensive analysis of panax ginseng root transcriptomes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464628/
https://www.ncbi.nlm.nih.gov/pubmed/26063328
http://dx.doi.org/10.1186/s12870-015-0527-0
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