EPR spectroscopy of chlorpromazine-induced free radical formation in normal human melanocytes

The purpose of this study was to estimate the effect of chlorpromazine on free radical concentration in HEMn-DP melanocytes using electron paramagnetic resonance (EPR) spectroscopy. It was found that chlorpromazine at concentrations of 1 × 10(−7) and 1 × 10(−6) M contributed to the formation of free...

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Autores principales: Otręba, Michał, Zdybel, Magdalena, Pilawa, Barbara, Beberok, Artur, Wrześniok, Dorota, Rok, Jakub, Buszman, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464733/
https://www.ncbi.nlm.nih.gov/pubmed/25981866
http://dx.doi.org/10.1007/s00249-015-1029-6
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author Otręba, Michał
Zdybel, Magdalena
Pilawa, Barbara
Beberok, Artur
Wrześniok, Dorota
Rok, Jakub
Buszman, Ewa
author_facet Otręba, Michał
Zdybel, Magdalena
Pilawa, Barbara
Beberok, Artur
Wrześniok, Dorota
Rok, Jakub
Buszman, Ewa
author_sort Otręba, Michał
collection PubMed
description The purpose of this study was to estimate the effect of chlorpromazine on free radical concentration in HEMn-DP melanocytes using electron paramagnetic resonance (EPR) spectroscopy. It was found that chlorpromazine at concentrations of 1 × 10(−7) and 1 × 10(−6) M contributed to the formation of free radicals (g values ~2) in a dose-dependent manner. The increase in free radical formation was accompanied by an increase in cytotoxicity, as shown by a tetrazolium assay. Homogeneous broadening of EPR lines, slow spin–lattice relaxation processes, and strong dipolar interactions characterized all the tested cellular samples. The performed examination of free radical formation in cells exposed to different chlorpromazine concentrations confirmed the usefulness of electron paramagnetic resonance spectroscopy to determine the effect of a drug on free radical production in a cellular model system in vitro.
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spelling pubmed-44647332015-06-17 EPR spectroscopy of chlorpromazine-induced free radical formation in normal human melanocytes Otręba, Michał Zdybel, Magdalena Pilawa, Barbara Beberok, Artur Wrześniok, Dorota Rok, Jakub Buszman, Ewa Eur Biophys J Original Paper The purpose of this study was to estimate the effect of chlorpromazine on free radical concentration in HEMn-DP melanocytes using electron paramagnetic resonance (EPR) spectroscopy. It was found that chlorpromazine at concentrations of 1 × 10(−7) and 1 × 10(−6) M contributed to the formation of free radicals (g values ~2) in a dose-dependent manner. The increase in free radical formation was accompanied by an increase in cytotoxicity, as shown by a tetrazolium assay. Homogeneous broadening of EPR lines, slow spin–lattice relaxation processes, and strong dipolar interactions characterized all the tested cellular samples. The performed examination of free radical formation in cells exposed to different chlorpromazine concentrations confirmed the usefulness of electron paramagnetic resonance spectroscopy to determine the effect of a drug on free radical production in a cellular model system in vitro. Springer Berlin Heidelberg 2015-05-16 2015 /pmc/articles/PMC4464733/ /pubmed/25981866 http://dx.doi.org/10.1007/s00249-015-1029-6 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Paper
Otręba, Michał
Zdybel, Magdalena
Pilawa, Barbara
Beberok, Artur
Wrześniok, Dorota
Rok, Jakub
Buszman, Ewa
EPR spectroscopy of chlorpromazine-induced free radical formation in normal human melanocytes
title EPR spectroscopy of chlorpromazine-induced free radical formation in normal human melanocytes
title_full EPR spectroscopy of chlorpromazine-induced free radical formation in normal human melanocytes
title_fullStr EPR spectroscopy of chlorpromazine-induced free radical formation in normal human melanocytes
title_full_unstemmed EPR spectroscopy of chlorpromazine-induced free radical formation in normal human melanocytes
title_short EPR spectroscopy of chlorpromazine-induced free radical formation in normal human melanocytes
title_sort epr spectroscopy of chlorpromazine-induced free radical formation in normal human melanocytes
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464733/
https://www.ncbi.nlm.nih.gov/pubmed/25981866
http://dx.doi.org/10.1007/s00249-015-1029-6
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