Cargando…

Prevalence and genetic diversity of noroviruses in adults with acute gastroenteritis in Huzhou, China, 2013–2014

Norovirus (NoV) infection is the most common cause of nonbacterial acute gastroenteritis, which affects both adults and children. However, the molecular epidemiology of NoV in adults with acute gastroenteritis in China has not been investigated extensively. In this study, we investigated the occurre...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Xiaofang, Han, Jiankang, Chen, Liping, Xu, Deshun, Shen, Yuehua, Zha, Yunfeng, Zhu, Xiaojuan, Ji, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464852/
https://www.ncbi.nlm.nih.gov/pubmed/25951970
http://dx.doi.org/10.1007/s00705-015-2440-0
_version_ 1782376036704452608
author Wu, Xiaofang
Han, Jiankang
Chen, Liping
Xu, Deshun
Shen, Yuehua
Zha, Yunfeng
Zhu, Xiaojuan
Ji, Lei
author_facet Wu, Xiaofang
Han, Jiankang
Chen, Liping
Xu, Deshun
Shen, Yuehua
Zha, Yunfeng
Zhu, Xiaojuan
Ji, Lei
author_sort Wu, Xiaofang
collection PubMed
description Norovirus (NoV) infection is the most common cause of nonbacterial acute gastroenteritis, which affects both adults and children. However, the molecular epidemiology of NoV in adults with acute gastroenteritis in China has not been investigated extensively. In this study, we investigated the occurrence of NoV infections and analyzed the genetic diversity of NoV in adults with acute gastroenteritis in Huzhou, China. A total of 796 fecal samples were collected from outpatients (≥16 years of age) between March 2013 and February 2014. Real-time RT-PCR was performed to detect NoV genogroups I (GI) and II (GII). For genotyping, the capsid and RNA-dependent RNA polymerase (RdRp) genes were partially amplified and sequenced for phylogenetic analysis. NoVs were detected in 26.51 % (211/796) of the specimens, with GII being predominant, representing 96.20 % of the NoV infections. At least nine genotypes were identified among GI and GII specimens, including GI.P2/GI.2, GI.P3/GI.3, GI.P4/GI.4, GII.Pe/GII.4 Sydney_2012, GII.P12/GII.3, GII.P7/GII.6, GII.P16/GII.13, GII.Pe, and GII.Pg (RdRp only). This is the first report of a GII.P16/GII.13 recombinant virus in adults in China. GII.Pe/GII.4 Sydney_2012 was the most prevalent genotype and the only GII.4 variant identified during the study period. Our findings suggested that NoV was a common causative agent of acute gastroenteritis in adults in Huzhou, China. During the study period, the NoVs circulating in adults in Huzhou were predominantly GII.4 Sydney_2012 variants and GII NoV recombinants.
format Online
Article
Text
id pubmed-4464852
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Springer Vienna
record_format MEDLINE/PubMed
spelling pubmed-44648522015-06-17 Prevalence and genetic diversity of noroviruses in adults with acute gastroenteritis in Huzhou, China, 2013–2014 Wu, Xiaofang Han, Jiankang Chen, Liping Xu, Deshun Shen, Yuehua Zha, Yunfeng Zhu, Xiaojuan Ji, Lei Arch Virol Original Article Norovirus (NoV) infection is the most common cause of nonbacterial acute gastroenteritis, which affects both adults and children. However, the molecular epidemiology of NoV in adults with acute gastroenteritis in China has not been investigated extensively. In this study, we investigated the occurrence of NoV infections and analyzed the genetic diversity of NoV in adults with acute gastroenteritis in Huzhou, China. A total of 796 fecal samples were collected from outpatients (≥16 years of age) between March 2013 and February 2014. Real-time RT-PCR was performed to detect NoV genogroups I (GI) and II (GII). For genotyping, the capsid and RNA-dependent RNA polymerase (RdRp) genes were partially amplified and sequenced for phylogenetic analysis. NoVs were detected in 26.51 % (211/796) of the specimens, with GII being predominant, representing 96.20 % of the NoV infections. At least nine genotypes were identified among GI and GII specimens, including GI.P2/GI.2, GI.P3/GI.3, GI.P4/GI.4, GII.Pe/GII.4 Sydney_2012, GII.P12/GII.3, GII.P7/GII.6, GII.P16/GII.13, GII.Pe, and GII.Pg (RdRp only). This is the first report of a GII.P16/GII.13 recombinant virus in adults in China. GII.Pe/GII.4 Sydney_2012 was the most prevalent genotype and the only GII.4 variant identified during the study period. Our findings suggested that NoV was a common causative agent of acute gastroenteritis in adults in Huzhou, China. During the study period, the NoVs circulating in adults in Huzhou were predominantly GII.4 Sydney_2012 variants and GII NoV recombinants. Springer Vienna 2015-05-08 2015 /pmc/articles/PMC4464852/ /pubmed/25951970 http://dx.doi.org/10.1007/s00705-015-2440-0 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Wu, Xiaofang
Han, Jiankang
Chen, Liping
Xu, Deshun
Shen, Yuehua
Zha, Yunfeng
Zhu, Xiaojuan
Ji, Lei
Prevalence and genetic diversity of noroviruses in adults with acute gastroenteritis in Huzhou, China, 2013–2014
title Prevalence and genetic diversity of noroviruses in adults with acute gastroenteritis in Huzhou, China, 2013–2014
title_full Prevalence and genetic diversity of noroviruses in adults with acute gastroenteritis in Huzhou, China, 2013–2014
title_fullStr Prevalence and genetic diversity of noroviruses in adults with acute gastroenteritis in Huzhou, China, 2013–2014
title_full_unstemmed Prevalence and genetic diversity of noroviruses in adults with acute gastroenteritis in Huzhou, China, 2013–2014
title_short Prevalence and genetic diversity of noroviruses in adults with acute gastroenteritis in Huzhou, China, 2013–2014
title_sort prevalence and genetic diversity of noroviruses in adults with acute gastroenteritis in huzhou, china, 2013–2014
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464852/
https://www.ncbi.nlm.nih.gov/pubmed/25951970
http://dx.doi.org/10.1007/s00705-015-2440-0
work_keys_str_mv AT wuxiaofang prevalenceandgeneticdiversityofnorovirusesinadultswithacutegastroenteritisinhuzhouchina20132014
AT hanjiankang prevalenceandgeneticdiversityofnorovirusesinadultswithacutegastroenteritisinhuzhouchina20132014
AT chenliping prevalenceandgeneticdiversityofnorovirusesinadultswithacutegastroenteritisinhuzhouchina20132014
AT xudeshun prevalenceandgeneticdiversityofnorovirusesinadultswithacutegastroenteritisinhuzhouchina20132014
AT shenyuehua prevalenceandgeneticdiversityofnorovirusesinadultswithacutegastroenteritisinhuzhouchina20132014
AT zhayunfeng prevalenceandgeneticdiversityofnorovirusesinadultswithacutegastroenteritisinhuzhouchina20132014
AT zhuxiaojuan prevalenceandgeneticdiversityofnorovirusesinadultswithacutegastroenteritisinhuzhouchina20132014
AT jilei prevalenceandgeneticdiversityofnorovirusesinadultswithacutegastroenteritisinhuzhouchina20132014