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High Rab27A expression indicates favorable prognosis in CRC

BACKGROUND: Rab27A is a peculiar member in Rab family and has been suggested to play essential roles in the development of human cancers. However, the association between Rab27A expression and clinicopathological characteristics of colorectal cancer (CRC) has not been elucidated yet. METHODS: One-st...

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Autores principales: Shi, Chuanbing, Yang, Xiaojun, Ni, Yijiang, Hou, Ning, Xu, Li, Zhan, Feng, Zhu, Huijun, Xiong, Lin, Chen, Pingsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4465473/
https://www.ncbi.nlm.nih.gov/pubmed/26070933
http://dx.doi.org/10.1186/s13000-015-0303-3
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author Shi, Chuanbing
Yang, Xiaojun
Ni, Yijiang
Hou, Ning
Xu, Li
Zhan, Feng
Zhu, Huijun
Xiong, Lin
Chen, Pingsheng
author_facet Shi, Chuanbing
Yang, Xiaojun
Ni, Yijiang
Hou, Ning
Xu, Li
Zhan, Feng
Zhu, Huijun
Xiong, Lin
Chen, Pingsheng
author_sort Shi, Chuanbing
collection PubMed
description BACKGROUND: Rab27A is a peculiar member in Rab family and has been suggested to play essential roles in the development of human cancers. However, the association between Rab27A expression and clinicopathological characteristics of colorectal cancer (CRC) has not been elucidated yet. METHODS: One-step quantitative real-time polymerase chain reaction (qPCR) test with 18 fresh-frozen CRC samples and immunohistochemistry (IHC) analysis in 112 CRC cases were executed to evaluate the relationship between Rab27A expression and the clinicopathological features of CRC. Cox regression and Kaplan-Meier survival analyses were performed to identify the prognostic factors for 112 CRC patients. RESULTS: The results specified that the expression levels of Rab27A mRNA and protein were significantly higher in CRC tissues than that in matched non-cancerous tissues, in both qPCR test (p = 0.029) and IHC analysis (p = 0.020). The IHC data indicated that the Rab27A protein expression in CRC was statistically correlated with lymph node metastasis (p = 0.022) and TNM stage (p = 0.026). Cox multi-factor analysis and Kaplan-Meier method suggested Rab27A protein expression (p = 0.012) and tumor differentiation (p = 0.004) were significantly associated with the overall survival of CRC patients. CONCLUSION: The data indicated the differentiate expression of Rab27A in CRC tissues and matched non-cancerous tissues. Rab27A may be used as a valuable prognostic biomarker for CRC patients.
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spelling pubmed-44654732015-06-15 High Rab27A expression indicates favorable prognosis in CRC Shi, Chuanbing Yang, Xiaojun Ni, Yijiang Hou, Ning Xu, Li Zhan, Feng Zhu, Huijun Xiong, Lin Chen, Pingsheng Diagn Pathol Research BACKGROUND: Rab27A is a peculiar member in Rab family and has been suggested to play essential roles in the development of human cancers. However, the association between Rab27A expression and clinicopathological characteristics of colorectal cancer (CRC) has not been elucidated yet. METHODS: One-step quantitative real-time polymerase chain reaction (qPCR) test with 18 fresh-frozen CRC samples and immunohistochemistry (IHC) analysis in 112 CRC cases were executed to evaluate the relationship between Rab27A expression and the clinicopathological features of CRC. Cox regression and Kaplan-Meier survival analyses were performed to identify the prognostic factors for 112 CRC patients. RESULTS: The results specified that the expression levels of Rab27A mRNA and protein were significantly higher in CRC tissues than that in matched non-cancerous tissues, in both qPCR test (p = 0.029) and IHC analysis (p = 0.020). The IHC data indicated that the Rab27A protein expression in CRC was statistically correlated with lymph node metastasis (p = 0.022) and TNM stage (p = 0.026). Cox multi-factor analysis and Kaplan-Meier method suggested Rab27A protein expression (p = 0.012) and tumor differentiation (p = 0.004) were significantly associated with the overall survival of CRC patients. CONCLUSION: The data indicated the differentiate expression of Rab27A in CRC tissues and matched non-cancerous tissues. Rab27A may be used as a valuable prognostic biomarker for CRC patients. BioMed Central 2015-06-13 /pmc/articles/PMC4465473/ /pubmed/26070933 http://dx.doi.org/10.1186/s13000-015-0303-3 Text en © Shi et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Research
Shi, Chuanbing
Yang, Xiaojun
Ni, Yijiang
Hou, Ning
Xu, Li
Zhan, Feng
Zhu, Huijun
Xiong, Lin
Chen, Pingsheng
High Rab27A expression indicates favorable prognosis in CRC
title High Rab27A expression indicates favorable prognosis in CRC
title_full High Rab27A expression indicates favorable prognosis in CRC
title_fullStr High Rab27A expression indicates favorable prognosis in CRC
title_full_unstemmed High Rab27A expression indicates favorable prognosis in CRC
title_short High Rab27A expression indicates favorable prognosis in CRC
title_sort high rab27a expression indicates favorable prognosis in crc
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4465473/
https://www.ncbi.nlm.nih.gov/pubmed/26070933
http://dx.doi.org/10.1186/s13000-015-0303-3
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