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Genotyping-By-Sequencing (GBS) Detects Genetic Structure and Confirms Behavioral QTL in Tame and Aggressive Foxes (Vulpes vulpes)
The silver fox (Vulpes vulpes) offers a novel model for studying the genetics of social behavior and animal domestication. Selection of foxes, separately, for tame and for aggressive behavior has yielded two strains with markedly different, genetically determined, behavioral phenotypes. Tame strain...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4465646/ https://www.ncbi.nlm.nih.gov/pubmed/26061395 http://dx.doi.org/10.1371/journal.pone.0127013 |
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author | Johnson, Jennifer L. Wittgenstein, Helena Mitchell, Sharon E. Hyma, Katie E. Temnykh, Svetlana V. Kharlamova, Anastasiya V. Gulevich, Rimma G. Vladimirova, Anastasiya V. Fong, Hiu Wa Flora Acland, Gregory M. Trut, Lyudmila N. Kukekova, Anna V. |
author_facet | Johnson, Jennifer L. Wittgenstein, Helena Mitchell, Sharon E. Hyma, Katie E. Temnykh, Svetlana V. Kharlamova, Anastasiya V. Gulevich, Rimma G. Vladimirova, Anastasiya V. Fong, Hiu Wa Flora Acland, Gregory M. Trut, Lyudmila N. Kukekova, Anna V. |
author_sort | Johnson, Jennifer L. |
collection | PubMed |
description | The silver fox (Vulpes vulpes) offers a novel model for studying the genetics of social behavior and animal domestication. Selection of foxes, separately, for tame and for aggressive behavior has yielded two strains with markedly different, genetically determined, behavioral phenotypes. Tame strain foxes are eager to establish human contact while foxes from the aggressive strain are aggressive and difficult to handle. These strains have been maintained as separate outbred lines for over 40 generations but their genetic structure has not been previously investigated. We applied a genotyping-by-sequencing (GBS) approach to provide insights into the genetic composition of these fox populations. Sequence analysis of EcoT22I genomic libraries of tame and aggressive foxes identified 48,294 high quality SNPs. Population structure analysis revealed genetic divergence between the two strains and more diversity in the aggressive strain than in the tame one. Significant differences in allele frequency between the strains were identified for 68 SNPs. Three of these SNPs were located on fox chromosome 14 within an interval of a previously identified behavioral QTL, further supporting the importance of this region for behavior. The GBS SNP data confirmed that significant genetic diversity has been preserved in both fox populations despite many years of selective breeding. Analysis of SNP allele frequencies in the two populations identified several regions of genetic divergence between the tame and aggressive foxes, some of which may represent targets of selection for behavior. The GBS protocol used in this study significantly expanded genomic resources for the fox, and can be adapted for SNP discovery and genotyping in other canid species. |
format | Online Article Text |
id | pubmed-4465646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44656462015-06-25 Genotyping-By-Sequencing (GBS) Detects Genetic Structure and Confirms Behavioral QTL in Tame and Aggressive Foxes (Vulpes vulpes) Johnson, Jennifer L. Wittgenstein, Helena Mitchell, Sharon E. Hyma, Katie E. Temnykh, Svetlana V. Kharlamova, Anastasiya V. Gulevich, Rimma G. Vladimirova, Anastasiya V. Fong, Hiu Wa Flora Acland, Gregory M. Trut, Lyudmila N. Kukekova, Anna V. PLoS One Research Article The silver fox (Vulpes vulpes) offers a novel model for studying the genetics of social behavior and animal domestication. Selection of foxes, separately, for tame and for aggressive behavior has yielded two strains with markedly different, genetically determined, behavioral phenotypes. Tame strain foxes are eager to establish human contact while foxes from the aggressive strain are aggressive and difficult to handle. These strains have been maintained as separate outbred lines for over 40 generations but their genetic structure has not been previously investigated. We applied a genotyping-by-sequencing (GBS) approach to provide insights into the genetic composition of these fox populations. Sequence analysis of EcoT22I genomic libraries of tame and aggressive foxes identified 48,294 high quality SNPs. Population structure analysis revealed genetic divergence between the two strains and more diversity in the aggressive strain than in the tame one. Significant differences in allele frequency between the strains were identified for 68 SNPs. Three of these SNPs were located on fox chromosome 14 within an interval of a previously identified behavioral QTL, further supporting the importance of this region for behavior. The GBS SNP data confirmed that significant genetic diversity has been preserved in both fox populations despite many years of selective breeding. Analysis of SNP allele frequencies in the two populations identified several regions of genetic divergence between the tame and aggressive foxes, some of which may represent targets of selection for behavior. The GBS protocol used in this study significantly expanded genomic resources for the fox, and can be adapted for SNP discovery and genotyping in other canid species. Public Library of Science 2015-06-10 /pmc/articles/PMC4465646/ /pubmed/26061395 http://dx.doi.org/10.1371/journal.pone.0127013 Text en © 2015 Johnson et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Johnson, Jennifer L. Wittgenstein, Helena Mitchell, Sharon E. Hyma, Katie E. Temnykh, Svetlana V. Kharlamova, Anastasiya V. Gulevich, Rimma G. Vladimirova, Anastasiya V. Fong, Hiu Wa Flora Acland, Gregory M. Trut, Lyudmila N. Kukekova, Anna V. Genotyping-By-Sequencing (GBS) Detects Genetic Structure and Confirms Behavioral QTL in Tame and Aggressive Foxes (Vulpes vulpes) |
title | Genotyping-By-Sequencing (GBS) Detects Genetic Structure and Confirms Behavioral QTL in Tame and Aggressive Foxes (Vulpes vulpes) |
title_full | Genotyping-By-Sequencing (GBS) Detects Genetic Structure and Confirms Behavioral QTL in Tame and Aggressive Foxes (Vulpes vulpes) |
title_fullStr | Genotyping-By-Sequencing (GBS) Detects Genetic Structure and Confirms Behavioral QTL in Tame and Aggressive Foxes (Vulpes vulpes) |
title_full_unstemmed | Genotyping-By-Sequencing (GBS) Detects Genetic Structure and Confirms Behavioral QTL in Tame and Aggressive Foxes (Vulpes vulpes) |
title_short | Genotyping-By-Sequencing (GBS) Detects Genetic Structure and Confirms Behavioral QTL in Tame and Aggressive Foxes (Vulpes vulpes) |
title_sort | genotyping-by-sequencing (gbs) detects genetic structure and confirms behavioral qtl in tame and aggressive foxes (vulpes vulpes) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4465646/ https://www.ncbi.nlm.nih.gov/pubmed/26061395 http://dx.doi.org/10.1371/journal.pone.0127013 |
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