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Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3
Chinese sacbrood virus (CSBV) is a small RNA virus family belonging to the genus Iflavirus that causes larval death, and even the collapse of entire bee colonies. The virus particle is spherical, non-enveloped, and its viral capsid is composed of four proteins, although the functions of the structur...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466328/ https://www.ncbi.nlm.nih.gov/pubmed/26067659 http://dx.doi.org/10.1371/journal.pone.0128486 |
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author | Fei, Dongliang Zhang, Haochun Diao, Qingyun Jiang, Lili Wang, Qiang Zhong, Yi Fan, Zhaobin Ma, Mingxiao |
author_facet | Fei, Dongliang Zhang, Haochun Diao, Qingyun Jiang, Lili Wang, Qiang Zhong, Yi Fan, Zhaobin Ma, Mingxiao |
author_sort | Fei, Dongliang |
collection | PubMed |
description | Chinese sacbrood virus (CSBV) is a small RNA virus family belonging to the genus Iflavirus that causes larval death, and even the collapse of entire bee colonies. The virus particle is spherical, non-enveloped, and its viral capsid is composed of four proteins, although the functions of the structural proteins are unclear. In this study, we used codon recoding to express the recombinant proteins VP1, VP2, and VP3 in Escherichia coli. SDS-PAGE analysis and Western blotting revealed that the target genes were expressed at high levels. Mice were then immunized with the purified, recombinant proteins, and antibody levels and lymphocyte proliferation were analyzed by ELISA and the MTT assay, respectively. The results show that the recombinant proteins induced high antibody levels and promoted lymphocyte proliferation. Polyclonal antibodies directed against these proteins will aid future studies of the molecular pathogenesis of CSBV. |
format | Online Article Text |
id | pubmed-4466328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44663282015-06-22 Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3 Fei, Dongliang Zhang, Haochun Diao, Qingyun Jiang, Lili Wang, Qiang Zhong, Yi Fan, Zhaobin Ma, Mingxiao PLoS One Research Article Chinese sacbrood virus (CSBV) is a small RNA virus family belonging to the genus Iflavirus that causes larval death, and even the collapse of entire bee colonies. The virus particle is spherical, non-enveloped, and its viral capsid is composed of four proteins, although the functions of the structural proteins are unclear. In this study, we used codon recoding to express the recombinant proteins VP1, VP2, and VP3 in Escherichia coli. SDS-PAGE analysis and Western blotting revealed that the target genes were expressed at high levels. Mice were then immunized with the purified, recombinant proteins, and antibody levels and lymphocyte proliferation were analyzed by ELISA and the MTT assay, respectively. The results show that the recombinant proteins induced high antibody levels and promoted lymphocyte proliferation. Polyclonal antibodies directed against these proteins will aid future studies of the molecular pathogenesis of CSBV. Public Library of Science 2015-06-12 /pmc/articles/PMC4466328/ /pubmed/26067659 http://dx.doi.org/10.1371/journal.pone.0128486 Text en © 2015 Fei et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Fei, Dongliang Zhang, Haochun Diao, Qingyun Jiang, Lili Wang, Qiang Zhong, Yi Fan, Zhaobin Ma, Mingxiao Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3 |
title | Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3 |
title_full | Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3 |
title_fullStr | Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3 |
title_full_unstemmed | Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3 |
title_short | Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3 |
title_sort | codon optimization, expression in escherichia coli, and immunogenicity of recombinant chinese sacbrood virus (csbv) structural proteins vp1, vp2, and vp3 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466328/ https://www.ncbi.nlm.nih.gov/pubmed/26067659 http://dx.doi.org/10.1371/journal.pone.0128486 |
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