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Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3

Chinese sacbrood virus (CSBV) is a small RNA virus family belonging to the genus Iflavirus that causes larval death, and even the collapse of entire bee colonies. The virus particle is spherical, non-enveloped, and its viral capsid is composed of four proteins, although the functions of the structur...

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Autores principales: Fei, Dongliang, Zhang, Haochun, Diao, Qingyun, Jiang, Lili, Wang, Qiang, Zhong, Yi, Fan, Zhaobin, Ma, Mingxiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466328/
https://www.ncbi.nlm.nih.gov/pubmed/26067659
http://dx.doi.org/10.1371/journal.pone.0128486
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author Fei, Dongliang
Zhang, Haochun
Diao, Qingyun
Jiang, Lili
Wang, Qiang
Zhong, Yi
Fan, Zhaobin
Ma, Mingxiao
author_facet Fei, Dongliang
Zhang, Haochun
Diao, Qingyun
Jiang, Lili
Wang, Qiang
Zhong, Yi
Fan, Zhaobin
Ma, Mingxiao
author_sort Fei, Dongliang
collection PubMed
description Chinese sacbrood virus (CSBV) is a small RNA virus family belonging to the genus Iflavirus that causes larval death, and even the collapse of entire bee colonies. The virus particle is spherical, non-enveloped, and its viral capsid is composed of four proteins, although the functions of the structural proteins are unclear. In this study, we used codon recoding to express the recombinant proteins VP1, VP2, and VP3 in Escherichia coli. SDS-PAGE analysis and Western blotting revealed that the target genes were expressed at high levels. Mice were then immunized with the purified, recombinant proteins, and antibody levels and lymphocyte proliferation were analyzed by ELISA and the MTT assay, respectively. The results show that the recombinant proteins induced high antibody levels and promoted lymphocyte proliferation. Polyclonal antibodies directed against these proteins will aid future studies of the molecular pathogenesis of CSBV.
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spelling pubmed-44663282015-06-22 Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3 Fei, Dongliang Zhang, Haochun Diao, Qingyun Jiang, Lili Wang, Qiang Zhong, Yi Fan, Zhaobin Ma, Mingxiao PLoS One Research Article Chinese sacbrood virus (CSBV) is a small RNA virus family belonging to the genus Iflavirus that causes larval death, and even the collapse of entire bee colonies. The virus particle is spherical, non-enveloped, and its viral capsid is composed of four proteins, although the functions of the structural proteins are unclear. In this study, we used codon recoding to express the recombinant proteins VP1, VP2, and VP3 in Escherichia coli. SDS-PAGE analysis and Western blotting revealed that the target genes were expressed at high levels. Mice were then immunized with the purified, recombinant proteins, and antibody levels and lymphocyte proliferation were analyzed by ELISA and the MTT assay, respectively. The results show that the recombinant proteins induced high antibody levels and promoted lymphocyte proliferation. Polyclonal antibodies directed against these proteins will aid future studies of the molecular pathogenesis of CSBV. Public Library of Science 2015-06-12 /pmc/articles/PMC4466328/ /pubmed/26067659 http://dx.doi.org/10.1371/journal.pone.0128486 Text en © 2015 Fei et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fei, Dongliang
Zhang, Haochun
Diao, Qingyun
Jiang, Lili
Wang, Qiang
Zhong, Yi
Fan, Zhaobin
Ma, Mingxiao
Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3
title Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3
title_full Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3
title_fullStr Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3
title_full_unstemmed Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3
title_short Codon Optimization, Expression in Escherichia coli, and Immunogenicity of Recombinant Chinese Sacbrood Virus (CSBV) Structural Proteins VP1, VP2, and VP3
title_sort codon optimization, expression in escherichia coli, and immunogenicity of recombinant chinese sacbrood virus (csbv) structural proteins vp1, vp2, and vp3
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466328/
https://www.ncbi.nlm.nih.gov/pubmed/26067659
http://dx.doi.org/10.1371/journal.pone.0128486
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