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Effects of Four Formulations of Prostaglandin Analogs on Eye Surface Cells. A Comparative Study

We evaluated the cytotoxic effects of four prostaglandin analogs (PGAs) used to treat glaucoma. First we established primary cultures of conjunctival stromal cells from healthy donors. Then cell cultures were incubated with different concentrations (0, 0.1, 1, 5, 25, 50 and 100%) of commercial formu...

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Autores principales: Pérez-Roca, Fernando, Rodrigo-Morales, Esther, Garzón, Ingrid, Oliveira, Ana-Celeste, Martín-Piedra, Miguel-Ángel, Carriel, Víctor, Ortiz-Pérez, Ana-Isabel, Sánchez-Montesinos, Indalecio, Campos, Antonio, Alaminos, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466352/
https://www.ncbi.nlm.nih.gov/pubmed/26067827
http://dx.doi.org/10.1371/journal.pone.0129419
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author Pérez-Roca, Fernando
Rodrigo-Morales, Esther
Garzón, Ingrid
Oliveira, Ana-Celeste
Martín-Piedra, Miguel-Ángel
Carriel, Víctor
Ortiz-Pérez, Ana-Isabel
Sánchez-Montesinos, Indalecio
Campos, Antonio
Alaminos, Miguel
author_facet Pérez-Roca, Fernando
Rodrigo-Morales, Esther
Garzón, Ingrid
Oliveira, Ana-Celeste
Martín-Piedra, Miguel-Ángel
Carriel, Víctor
Ortiz-Pérez, Ana-Isabel
Sánchez-Montesinos, Indalecio
Campos, Antonio
Alaminos, Miguel
author_sort Pérez-Roca, Fernando
collection PubMed
description We evaluated the cytotoxic effects of four prostaglandin analogs (PGAs) used to treat glaucoma. First we established primary cultures of conjunctival stromal cells from healthy donors. Then cell cultures were incubated with different concentrations (0, 0.1, 1, 5, 25, 50 and 100%) of commercial formulations of bimatoprost, tafluprost, travoprost and latanoprost for increasing periods (5 and 30 min, 1 h, 6 h and 24 h) and cell survival was assessed with three different methods: WST-1, MTT and calcein/AM-ethidium homodimer-1 assays. Our results showed that all PGAs were associated with a certain level of cell damage, which correlated significantly with the concentration of PGA used, and to a lesser extent with culture time. Tafluprost tended to be less toxic than bimatoprost, travoprost and latanoprost after all culture periods. The results for WST-1, MTT and calcein/AM-ethidium homodimer-1 correlated closely. When the average lethal dose 50 was calculated, we found that the most cytotoxic drug was latanoprost, whereas tafluprost was the most sparing of the ocular surface in vitro. These results indicate the need to design novel PGAs with high effectiveness but free from the cytotoxic effects that we found, or at least to obtain drugs that are functional at low dosages. The fact that the commercial formulation of tafluprost used in this work was preservative-free may support the current tendency to eliminate preservatives from eye drops for clinical use.
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spelling pubmed-44663522015-06-22 Effects of Four Formulations of Prostaglandin Analogs on Eye Surface Cells. A Comparative Study Pérez-Roca, Fernando Rodrigo-Morales, Esther Garzón, Ingrid Oliveira, Ana-Celeste Martín-Piedra, Miguel-Ángel Carriel, Víctor Ortiz-Pérez, Ana-Isabel Sánchez-Montesinos, Indalecio Campos, Antonio Alaminos, Miguel PLoS One Research Article We evaluated the cytotoxic effects of four prostaglandin analogs (PGAs) used to treat glaucoma. First we established primary cultures of conjunctival stromal cells from healthy donors. Then cell cultures were incubated with different concentrations (0, 0.1, 1, 5, 25, 50 and 100%) of commercial formulations of bimatoprost, tafluprost, travoprost and latanoprost for increasing periods (5 and 30 min, 1 h, 6 h and 24 h) and cell survival was assessed with three different methods: WST-1, MTT and calcein/AM-ethidium homodimer-1 assays. Our results showed that all PGAs were associated with a certain level of cell damage, which correlated significantly with the concentration of PGA used, and to a lesser extent with culture time. Tafluprost tended to be less toxic than bimatoprost, travoprost and latanoprost after all culture periods. The results for WST-1, MTT and calcein/AM-ethidium homodimer-1 correlated closely. When the average lethal dose 50 was calculated, we found that the most cytotoxic drug was latanoprost, whereas tafluprost was the most sparing of the ocular surface in vitro. These results indicate the need to design novel PGAs with high effectiveness but free from the cytotoxic effects that we found, or at least to obtain drugs that are functional at low dosages. The fact that the commercial formulation of tafluprost used in this work was preservative-free may support the current tendency to eliminate preservatives from eye drops for clinical use. Public Library of Science 2015-06-12 /pmc/articles/PMC4466352/ /pubmed/26067827 http://dx.doi.org/10.1371/journal.pone.0129419 Text en © 2015 Pérez-Roca et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pérez-Roca, Fernando
Rodrigo-Morales, Esther
Garzón, Ingrid
Oliveira, Ana-Celeste
Martín-Piedra, Miguel-Ángel
Carriel, Víctor
Ortiz-Pérez, Ana-Isabel
Sánchez-Montesinos, Indalecio
Campos, Antonio
Alaminos, Miguel
Effects of Four Formulations of Prostaglandin Analogs on Eye Surface Cells. A Comparative Study
title Effects of Four Formulations of Prostaglandin Analogs on Eye Surface Cells. A Comparative Study
title_full Effects of Four Formulations of Prostaglandin Analogs on Eye Surface Cells. A Comparative Study
title_fullStr Effects of Four Formulations of Prostaglandin Analogs on Eye Surface Cells. A Comparative Study
title_full_unstemmed Effects of Four Formulations of Prostaglandin Analogs on Eye Surface Cells. A Comparative Study
title_short Effects of Four Formulations of Prostaglandin Analogs on Eye Surface Cells. A Comparative Study
title_sort effects of four formulations of prostaglandin analogs on eye surface cells. a comparative study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466352/
https://www.ncbi.nlm.nih.gov/pubmed/26067827
http://dx.doi.org/10.1371/journal.pone.0129419
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