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Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone

Though benign, giant cell tumor of bone (GCTB) can become aggressive and can exhibit a high mitotic rate, necrosis and rarely vascular invasion and metastasis. GCTB has unique histologic characteristics, a high rate of multinucleated cells, a variable and unpredictable growth potential and uncertain...

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Autores principales: Conceição, André Luis Giacometti, Babeto, Erica, Candido, Natalia Maria, Franco, Fernanda Craveiro, de Campos Zuccari, Débora Aparecida Pires, Bonilha, Jane Lopes, Cordeiro, José Antônio, Calmon, Marilia Freitas, Rahal, Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466407/
https://www.ncbi.nlm.nih.gov/pubmed/26078788
http://dx.doi.org/10.7150/jca.11238
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author Conceição, André Luis Giacometti
Babeto, Erica
Candido, Natalia Maria
Franco, Fernanda Craveiro
de Campos Zuccari, Débora Aparecida Pires
Bonilha, Jane Lopes
Cordeiro, José Antônio
Calmon, Marilia Freitas
Rahal, Paula
author_facet Conceição, André Luis Giacometti
Babeto, Erica
Candido, Natalia Maria
Franco, Fernanda Craveiro
de Campos Zuccari, Débora Aparecida Pires
Bonilha, Jane Lopes
Cordeiro, José Antônio
Calmon, Marilia Freitas
Rahal, Paula
author_sort Conceição, André Luis Giacometti
collection PubMed
description Though benign, giant cell tumor of bone (GCTB) can become aggressive and can exhibit a high mitotic rate, necrosis and rarely vascular invasion and metastasis. GCTB has unique histologic characteristics, a high rate of multinucleated cells, a variable and unpredictable growth potential and uncertain biological behavior. In this study, we sought to identify genes differentially expressed in GCTB, thus building a molecular profile of this tumor. We performed quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry and analyses of methylation to identify genes that are putatively associated with GCTB. The expression of the ADAM23 and CDKN2A genes was decreased in GCTB samples compared to normal bone tissue, measured by qPCR. Additionally, a high hypermethylation frequency of the promoter regions of ADAM23 and CDKN2A in GCTB was observed. The expression of the MAP2K3, MMP14, TIMP2 and VIM genes was significantly higher in GCTB than in normal bone tissue, a fact that was confirmed by qPCR and immunohistochemistry. The set of genes identified here furthers our understanding of the molecular basis of GCTB.
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spelling pubmed-44664072015-06-15 Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone Conceição, André Luis Giacometti Babeto, Erica Candido, Natalia Maria Franco, Fernanda Craveiro de Campos Zuccari, Débora Aparecida Pires Bonilha, Jane Lopes Cordeiro, José Antônio Calmon, Marilia Freitas Rahal, Paula J Cancer Research Paper Though benign, giant cell tumor of bone (GCTB) can become aggressive and can exhibit a high mitotic rate, necrosis and rarely vascular invasion and metastasis. GCTB has unique histologic characteristics, a high rate of multinucleated cells, a variable and unpredictable growth potential and uncertain biological behavior. In this study, we sought to identify genes differentially expressed in GCTB, thus building a molecular profile of this tumor. We performed quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry and analyses of methylation to identify genes that are putatively associated with GCTB. The expression of the ADAM23 and CDKN2A genes was decreased in GCTB samples compared to normal bone tissue, measured by qPCR. Additionally, a high hypermethylation frequency of the promoter regions of ADAM23 and CDKN2A in GCTB was observed. The expression of the MAP2K3, MMP14, TIMP2 and VIM genes was significantly higher in GCTB than in normal bone tissue, a fact that was confirmed by qPCR and immunohistochemistry. The set of genes identified here furthers our understanding of the molecular basis of GCTB. Ivyspring International Publisher 2015-05-23 /pmc/articles/PMC4466407/ /pubmed/26078788 http://dx.doi.org/10.7150/jca.11238 Text en © 2015 Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions.
spellingShingle Research Paper
Conceição, André Luis Giacometti
Babeto, Erica
Candido, Natalia Maria
Franco, Fernanda Craveiro
de Campos Zuccari, Débora Aparecida Pires
Bonilha, Jane Lopes
Cordeiro, José Antônio
Calmon, Marilia Freitas
Rahal, Paula
Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone
title Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone
title_full Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone
title_fullStr Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone
title_full_unstemmed Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone
title_short Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone
title_sort differential expression of adam23, cdkn2a (p16), mmp14 and vim associated with giant cell tumor of bone
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466407/
https://www.ncbi.nlm.nih.gov/pubmed/26078788
http://dx.doi.org/10.7150/jca.11238
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