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Analysis of the type II-A CRISPR-Cas system of Streptococcus agalactiae reveals distinctive features according to genetic lineages

CRISPR-Cas systems (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) are found in 90% of archaea and about 40% of bacteria. In this original system, CRISPR arrays comprise short, almost unique sequences called spacers that are interspersed with conserved palindro...

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Autores principales: Lier, Clément, Baticle, Elodie, Horvath, Philippe, Haguenoer, Eve, Valentin, Anne-Sophie, Glaser, Philippe, Mereghetti, Laurent, Lanotte, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466440/
https://www.ncbi.nlm.nih.gov/pubmed/26124774
http://dx.doi.org/10.3389/fgene.2015.00214
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author Lier, Clément
Baticle, Elodie
Horvath, Philippe
Haguenoer, Eve
Valentin, Anne-Sophie
Glaser, Philippe
Mereghetti, Laurent
Lanotte, Philippe
author_facet Lier, Clément
Baticle, Elodie
Horvath, Philippe
Haguenoer, Eve
Valentin, Anne-Sophie
Glaser, Philippe
Mereghetti, Laurent
Lanotte, Philippe
author_sort Lier, Clément
collection PubMed
description CRISPR-Cas systems (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) are found in 90% of archaea and about 40% of bacteria. In this original system, CRISPR arrays comprise short, almost unique sequences called spacers that are interspersed with conserved palindromic repeats. These systems play a role in adaptive immunity and participate to fight non-self DNA such as integrative and conjugative elements, plasmids, and phages. In Streptococcus agalactiae, a bacterium implicated in colonization and infections in humans since the 1960s, two CRISPR-Cas systems have been described. A type II-A system, characterized by proteins Cas9, Cas1, Cas2, and Csn2, is ubiquitous, and a type I–C system, with the Cas8c signature protein, is present in about 20% of the isolates. Unlike type I–C, which appears to be non-functional, type II-A appears fully functional. Here we studied type II-A CRISPR-cas loci from 126 human isolates of S. agalactiae belonging to different clonal complexes that represent the diversity of the species and that have been implicated in colonization or infection. The CRISPR-cas locus was analyzed both at spacer and repeat levels. Major distinctive features were identified according to the phylogenetic lineages previously defined by multilocus sequence typing, especially for the sequence type (ST) 17, which is considered hypervirulent. Among other idiosyncrasies, ST-17 shows a significantly lower number of spacers in comparison with other lineages. This characteristic could reflect the peculiar virulence or colonization specificities of this lineage.
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spelling pubmed-44664402015-06-29 Analysis of the type II-A CRISPR-Cas system of Streptococcus agalactiae reveals distinctive features according to genetic lineages Lier, Clément Baticle, Elodie Horvath, Philippe Haguenoer, Eve Valentin, Anne-Sophie Glaser, Philippe Mereghetti, Laurent Lanotte, Philippe Front Genet Genetics CRISPR-Cas systems (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) are found in 90% of archaea and about 40% of bacteria. In this original system, CRISPR arrays comprise short, almost unique sequences called spacers that are interspersed with conserved palindromic repeats. These systems play a role in adaptive immunity and participate to fight non-self DNA such as integrative and conjugative elements, plasmids, and phages. In Streptococcus agalactiae, a bacterium implicated in colonization and infections in humans since the 1960s, two CRISPR-Cas systems have been described. A type II-A system, characterized by proteins Cas9, Cas1, Cas2, and Csn2, is ubiquitous, and a type I–C system, with the Cas8c signature protein, is present in about 20% of the isolates. Unlike type I–C, which appears to be non-functional, type II-A appears fully functional. Here we studied type II-A CRISPR-cas loci from 126 human isolates of S. agalactiae belonging to different clonal complexes that represent the diversity of the species and that have been implicated in colonization or infection. The CRISPR-cas locus was analyzed both at spacer and repeat levels. Major distinctive features were identified according to the phylogenetic lineages previously defined by multilocus sequence typing, especially for the sequence type (ST) 17, which is considered hypervirulent. Among other idiosyncrasies, ST-17 shows a significantly lower number of spacers in comparison with other lineages. This characteristic could reflect the peculiar virulence or colonization specificities of this lineage. Frontiers Media S.A. 2015-06-15 /pmc/articles/PMC4466440/ /pubmed/26124774 http://dx.doi.org/10.3389/fgene.2015.00214 Text en Copyright © 2015 Lier, Baticle, Horvath, Haguenoer, Valentin, Glaser, Mereghetti and Lanotte. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Lier, Clément
Baticle, Elodie
Horvath, Philippe
Haguenoer, Eve
Valentin, Anne-Sophie
Glaser, Philippe
Mereghetti, Laurent
Lanotte, Philippe
Analysis of the type II-A CRISPR-Cas system of Streptococcus agalactiae reveals distinctive features according to genetic lineages
title Analysis of the type II-A CRISPR-Cas system of Streptococcus agalactiae reveals distinctive features according to genetic lineages
title_full Analysis of the type II-A CRISPR-Cas system of Streptococcus agalactiae reveals distinctive features according to genetic lineages
title_fullStr Analysis of the type II-A CRISPR-Cas system of Streptococcus agalactiae reveals distinctive features according to genetic lineages
title_full_unstemmed Analysis of the type II-A CRISPR-Cas system of Streptococcus agalactiae reveals distinctive features according to genetic lineages
title_short Analysis of the type II-A CRISPR-Cas system of Streptococcus agalactiae reveals distinctive features according to genetic lineages
title_sort analysis of the type ii-a crispr-cas system of streptococcus agalactiae reveals distinctive features according to genetic lineages
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466440/
https://www.ncbi.nlm.nih.gov/pubmed/26124774
http://dx.doi.org/10.3389/fgene.2015.00214
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