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Gene amplification during differentiation of mammalian neural stem cells in vitro and in vivo

In development of amphibians and flies, gene amplification is one of mechanisms to increase gene expression. In mammalian cells, gene amplification seems to be restricted to tumorigenesis and acquiring of drug-resistance in cancer cells. Here, we report a complex gene amplification pattern in mouse...

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Autores principales: Fischer, Ulrike, Backes, Christina, Raslan, Abdulrahman, Keller, Andreas, Meier, Carola, Meese, Eckart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466667/
https://www.ncbi.nlm.nih.gov/pubmed/25760141
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author Fischer, Ulrike
Backes, Christina
Raslan, Abdulrahman
Keller, Andreas
Meier, Carola
Meese, Eckart
author_facet Fischer, Ulrike
Backes, Christina
Raslan, Abdulrahman
Keller, Andreas
Meier, Carola
Meese, Eckart
author_sort Fischer, Ulrike
collection PubMed
description In development of amphibians and flies, gene amplification is one of mechanisms to increase gene expression. In mammalian cells, gene amplification seems to be restricted to tumorigenesis and acquiring of drug-resistance in cancer cells. Here, we report a complex gene amplification pattern in mouse neural progenitor cells during differentiation with approximately 10% of the genome involved. Half of the amplified mouse chromosome regions overlap with amplified regions previously reported in human neural progenitor cells, indicating conserved mechanisms during differentiation. Using fluorescence in situ hybridization, we verified the amplification in single cells of primary mouse mesencephalon E14 (embryonic stage) neurosphere cells during differentiation. In vivo we confirmed gene amplifications of the TRP53 gene in cryosections from mouse embryos at stage E11.5. Gene amplification is not only a cancer-related mechanism but is also conserved in evolution, occurring during differentiation of mammalian neural stem cells
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spelling pubmed-44666672015-06-22 Gene amplification during differentiation of mammalian neural stem cells in vitro and in vivo Fischer, Ulrike Backes, Christina Raslan, Abdulrahman Keller, Andreas Meier, Carola Meese, Eckart Oncotarget Research Paper In development of amphibians and flies, gene amplification is one of mechanisms to increase gene expression. In mammalian cells, gene amplification seems to be restricted to tumorigenesis and acquiring of drug-resistance in cancer cells. Here, we report a complex gene amplification pattern in mouse neural progenitor cells during differentiation with approximately 10% of the genome involved. Half of the amplified mouse chromosome regions overlap with amplified regions previously reported in human neural progenitor cells, indicating conserved mechanisms during differentiation. Using fluorescence in situ hybridization, we verified the amplification in single cells of primary mouse mesencephalon E14 (embryonic stage) neurosphere cells during differentiation. In vivo we confirmed gene amplifications of the TRP53 gene in cryosections from mouse embryos at stage E11.5. Gene amplification is not only a cancer-related mechanism but is also conserved in evolution, occurring during differentiation of mammalian neural stem cells Impact Journals LLC 2015-02-19 /pmc/articles/PMC4466667/ /pubmed/25760141 Text en Copyright: © 2015 Fischer et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Fischer, Ulrike
Backes, Christina
Raslan, Abdulrahman
Keller, Andreas
Meier, Carola
Meese, Eckart
Gene amplification during differentiation of mammalian neural stem cells in vitro and in vivo
title Gene amplification during differentiation of mammalian neural stem cells in vitro and in vivo
title_full Gene amplification during differentiation of mammalian neural stem cells in vitro and in vivo
title_fullStr Gene amplification during differentiation of mammalian neural stem cells in vitro and in vivo
title_full_unstemmed Gene amplification during differentiation of mammalian neural stem cells in vitro and in vivo
title_short Gene amplification during differentiation of mammalian neural stem cells in vitro and in vivo
title_sort gene amplification during differentiation of mammalian neural stem cells in vitro and in vivo
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466667/
https://www.ncbi.nlm.nih.gov/pubmed/25760141
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