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Induction of rat hepatic mitochondrial membrane permeability transition pore opening by leaf extract of Olax subscorpioidea
BACKGROUND: The induction of the mitochondrial membrane permeability transition (MMPT) pore has been implicated in the cascade of events involved in apoptosis (programmed cell death). Olax subscorpioidea is traditionally used for the treatment of several diseases and infection. However, its role on...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466771/ https://www.ncbi.nlm.nih.gov/pubmed/26109790 http://dx.doi.org/10.4103/0974-8490.157998 |
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author | Adegbite, Oluwatobi Samuel Akinsanya, Yetunde Ifeoma Kukoyi, Ayobami Jahdahunsi Iyanda-Joel, Wisdom O. Daniel, Oluwatoyin O. Adebayo, Abiodun Humphrey |
author_facet | Adegbite, Oluwatobi Samuel Akinsanya, Yetunde Ifeoma Kukoyi, Ayobami Jahdahunsi Iyanda-Joel, Wisdom O. Daniel, Oluwatoyin O. Adebayo, Abiodun Humphrey |
author_sort | Adegbite, Oluwatobi Samuel |
collection | PubMed |
description | BACKGROUND: The induction of the mitochondrial membrane permeability transition (MMPT) pore has been implicated in the cascade of events involved in apoptosis (programmed cell death). Olax subscorpioidea is traditionally used for the treatment of several diseases and infection. However, its role on MMPT is not yet established. This study was aimed at evaluating the effects of varying concentrations of the methanol leaf extract of O. subscorpioidea (MEOS) on MMPT pore opening, mitochondrial adenosine triphosphatase (ATPase), and mitochondrial lipid peroxidation. MATERIALS AND METHODS: Opening of the pore was spectrophotometrically assayed under succinate-energized conditions. RESULTS: In the absence of triggering agent (calcium), MEOS induced MMPT pore opening by 350, 612, 827, 845% at 36, 60, 86 and 112 μg/ml, respectively. MEOS further induced MMPT pore opening in the presence of a triggering agent by 866, 905, 831, 840, 949% at 12, 36, 60, 86 and 112 μg/ml, respectively. The extract significantly induced mitochondrial membrane lipid peroxidation in all the concentration used. MEOS also significantly increased mitochondrial ATP hydrolysis by mitochondrial ATPase in all concentration of the extract used. CONCLUSION: It may be deduced from this results, that MEOS contains certain bioactive components that may find use in pathological conditions that require an enhanced rate of apoptosis. |
format | Online Article Text |
id | pubmed-4466771 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-44667712015-06-24 Induction of rat hepatic mitochondrial membrane permeability transition pore opening by leaf extract of Olax subscorpioidea Adegbite, Oluwatobi Samuel Akinsanya, Yetunde Ifeoma Kukoyi, Ayobami Jahdahunsi Iyanda-Joel, Wisdom O. Daniel, Oluwatoyin O. Adebayo, Abiodun Humphrey Pharmacognosy Res Original Article BACKGROUND: The induction of the mitochondrial membrane permeability transition (MMPT) pore has been implicated in the cascade of events involved in apoptosis (programmed cell death). Olax subscorpioidea is traditionally used for the treatment of several diseases and infection. However, its role on MMPT is not yet established. This study was aimed at evaluating the effects of varying concentrations of the methanol leaf extract of O. subscorpioidea (MEOS) on MMPT pore opening, mitochondrial adenosine triphosphatase (ATPase), and mitochondrial lipid peroxidation. MATERIALS AND METHODS: Opening of the pore was spectrophotometrically assayed under succinate-energized conditions. RESULTS: In the absence of triggering agent (calcium), MEOS induced MMPT pore opening by 350, 612, 827, 845% at 36, 60, 86 and 112 μg/ml, respectively. MEOS further induced MMPT pore opening in the presence of a triggering agent by 866, 905, 831, 840, 949% at 12, 36, 60, 86 and 112 μg/ml, respectively. The extract significantly induced mitochondrial membrane lipid peroxidation in all the concentration used. MEOS also significantly increased mitochondrial ATP hydrolysis by mitochondrial ATPase in all concentration of the extract used. CONCLUSION: It may be deduced from this results, that MEOS contains certain bioactive components that may find use in pathological conditions that require an enhanced rate of apoptosis. Medknow Publications & Media Pvt Ltd 2015-06 /pmc/articles/PMC4466771/ /pubmed/26109790 http://dx.doi.org/10.4103/0974-8490.157998 Text en Copyright: © Pharmacognosy Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Adegbite, Oluwatobi Samuel Akinsanya, Yetunde Ifeoma Kukoyi, Ayobami Jahdahunsi Iyanda-Joel, Wisdom O. Daniel, Oluwatoyin O. Adebayo, Abiodun Humphrey Induction of rat hepatic mitochondrial membrane permeability transition pore opening by leaf extract of Olax subscorpioidea |
title | Induction of rat hepatic mitochondrial membrane permeability transition pore opening by leaf extract of Olax subscorpioidea |
title_full | Induction of rat hepatic mitochondrial membrane permeability transition pore opening by leaf extract of Olax subscorpioidea |
title_fullStr | Induction of rat hepatic mitochondrial membrane permeability transition pore opening by leaf extract of Olax subscorpioidea |
title_full_unstemmed | Induction of rat hepatic mitochondrial membrane permeability transition pore opening by leaf extract of Olax subscorpioidea |
title_short | Induction of rat hepatic mitochondrial membrane permeability transition pore opening by leaf extract of Olax subscorpioidea |
title_sort | induction of rat hepatic mitochondrial membrane permeability transition pore opening by leaf extract of olax subscorpioidea |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466771/ https://www.ncbi.nlm.nih.gov/pubmed/26109790 http://dx.doi.org/10.4103/0974-8490.157998 |
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