Steady-state cross-correlations for live two-colour super-resolution localization data sets

Cross-correlation of super-resolution images gathered from point localizations allows for robust quantification of protein co-distributions in chemically fixed cells. Here this is extended to dynamic systems through an analysis that quantifies the steady-state cross-correlation between spectrally di...

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Detalles Bibliográficos
Autores principales: Stone, Matthew B., Veatch, Sarah L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467025/
https://www.ncbi.nlm.nih.gov/pubmed/26066572
http://dx.doi.org/10.1038/ncomms8347
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author Stone, Matthew B.
Veatch, Sarah L.
author_facet Stone, Matthew B.
Veatch, Sarah L.
author_sort Stone, Matthew B.
collection PubMed
description Cross-correlation of super-resolution images gathered from point localizations allows for robust quantification of protein co-distributions in chemically fixed cells. Here this is extended to dynamic systems through an analysis that quantifies the steady-state cross-correlation between spectrally distinguishable probes. This methodology is used to quantify the co-distribution of several mobile membrane proteins in both vesicles and live cells, including Lyn kinase and the B-cell receptor during antigen stimulation.
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spelling pubmed-44670252015-07-13 Steady-state cross-correlations for live two-colour super-resolution localization data sets Stone, Matthew B. Veatch, Sarah L. Nat Commun Article Cross-correlation of super-resolution images gathered from point localizations allows for robust quantification of protein co-distributions in chemically fixed cells. Here this is extended to dynamic systems through an analysis that quantifies the steady-state cross-correlation between spectrally distinguishable probes. This methodology is used to quantify the co-distribution of several mobile membrane proteins in both vesicles and live cells, including Lyn kinase and the B-cell receptor during antigen stimulation. Nature Pub. Group 2015-06-12 /pmc/articles/PMC4467025/ /pubmed/26066572 http://dx.doi.org/10.1038/ncomms8347 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Stone, Matthew B.
Veatch, Sarah L.
Steady-state cross-correlations for live two-colour super-resolution localization data sets
title Steady-state cross-correlations for live two-colour super-resolution localization data sets
title_full Steady-state cross-correlations for live two-colour super-resolution localization data sets
title_fullStr Steady-state cross-correlations for live two-colour super-resolution localization data sets
title_full_unstemmed Steady-state cross-correlations for live two-colour super-resolution localization data sets
title_short Steady-state cross-correlations for live two-colour super-resolution localization data sets
title_sort steady-state cross-correlations for live two-colour super-resolution localization data sets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467025/
https://www.ncbi.nlm.nih.gov/pubmed/26066572
http://dx.doi.org/10.1038/ncomms8347
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