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Small non-coding RNA deregulation in endometrial carcinogenesis

Small non-coding RNAs (sncRNAs) represent a heterogeneous group of <200nt-long transcripts comprising microRNAs, PIWI-interacting RNAs (piRNAs) and small-nucleolar-RNAs (snoRNAs) involved in physiological and pathological processes such as carcinogenesis and tumor progression. Aberrant sncRNA exp...

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Autores principales: Ravo, Maria, Cordella, Angela, Rinaldi, Antonio, Bruno, Giuseppina, Alexandrova, Elena, Saggese, Pasquale, Nassa, Giovanni, Giurato, Giorgio, Tarallo, Roberta, Marchese, Giovanna, Rizzo, Francesca, Stellato, Claudia, Biancardi, Rossella, Troisi, Jacopo, Di Spiezio Sardo, Attilio, Zullo, Fulvio, Weisz, Alessandro, Guida, Maurizio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467107/
https://www.ncbi.nlm.nih.gov/pubmed/25686835
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author Ravo, Maria
Cordella, Angela
Rinaldi, Antonio
Bruno, Giuseppina
Alexandrova, Elena
Saggese, Pasquale
Nassa, Giovanni
Giurato, Giorgio
Tarallo, Roberta
Marchese, Giovanna
Rizzo, Francesca
Stellato, Claudia
Biancardi, Rossella
Troisi, Jacopo
Di Spiezio Sardo, Attilio
Zullo, Fulvio
Weisz, Alessandro
Guida, Maurizio
author_facet Ravo, Maria
Cordella, Angela
Rinaldi, Antonio
Bruno, Giuseppina
Alexandrova, Elena
Saggese, Pasquale
Nassa, Giovanni
Giurato, Giorgio
Tarallo, Roberta
Marchese, Giovanna
Rizzo, Francesca
Stellato, Claudia
Biancardi, Rossella
Troisi, Jacopo
Di Spiezio Sardo, Attilio
Zullo, Fulvio
Weisz, Alessandro
Guida, Maurizio
author_sort Ravo, Maria
collection PubMed
description Small non-coding RNAs (sncRNAs) represent a heterogeneous group of <200nt-long transcripts comprising microRNAs, PIWI-interacting RNAs (piRNAs) and small-nucleolar-RNAs (snoRNAs) involved in physiological and pathological processes such as carcinogenesis and tumor progression. Aberrant sncRNA expression in cancer has been associated with specific clinical phenotypes, grading, staging, metastases development and resistance to therapy. Aim of the present work is to study the role of sncRNAs in endometrial carcinogenesis. Changes in sncRNA expression were identified by high-throughput genomic analysis of paired normal, hyperplastic and cancerous endometrial tissues obtained by endometrial biopsies (n = 10). Using smallRNA sequencing and microarrays we identified significant differences in sncRNA expression pattern between normal, hyperplastic and neoplastic endometrium. This led to the definition of a sncRNA signature (129 microRNAs, 2 of which not previously described, 10 piRNAs and 3 snoRNAs) of neoplastic transformation. Functional bioinformatics analysis identified as downstream targets multiple signaling pathways potentially involved in the hyperplastic and neoplastic tissue responses, including Wnt/β-catenin, and ERK/MAPK and TGF-β-Signaling. Considering the regulatory role of sncRNAs, this newly identified sncRNA signature is likely to reflect the events leading to endometrial cancer, which can be exploited to dissect the carcinogenic process including novel biomarkers for early and non-invasive diagnosis of these tumors.
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spelling pubmed-44671072015-06-22 Small non-coding RNA deregulation in endometrial carcinogenesis Ravo, Maria Cordella, Angela Rinaldi, Antonio Bruno, Giuseppina Alexandrova, Elena Saggese, Pasquale Nassa, Giovanni Giurato, Giorgio Tarallo, Roberta Marchese, Giovanna Rizzo, Francesca Stellato, Claudia Biancardi, Rossella Troisi, Jacopo Di Spiezio Sardo, Attilio Zullo, Fulvio Weisz, Alessandro Guida, Maurizio Oncotarget Research Paper Small non-coding RNAs (sncRNAs) represent a heterogeneous group of <200nt-long transcripts comprising microRNAs, PIWI-interacting RNAs (piRNAs) and small-nucleolar-RNAs (snoRNAs) involved in physiological and pathological processes such as carcinogenesis and tumor progression. Aberrant sncRNA expression in cancer has been associated with specific clinical phenotypes, grading, staging, metastases development and resistance to therapy. Aim of the present work is to study the role of sncRNAs in endometrial carcinogenesis. Changes in sncRNA expression were identified by high-throughput genomic analysis of paired normal, hyperplastic and cancerous endometrial tissues obtained by endometrial biopsies (n = 10). Using smallRNA sequencing and microarrays we identified significant differences in sncRNA expression pattern between normal, hyperplastic and neoplastic endometrium. This led to the definition of a sncRNA signature (129 microRNAs, 2 of which not previously described, 10 piRNAs and 3 snoRNAs) of neoplastic transformation. Functional bioinformatics analysis identified as downstream targets multiple signaling pathways potentially involved in the hyperplastic and neoplastic tissue responses, including Wnt/β-catenin, and ERK/MAPK and TGF-β-Signaling. Considering the regulatory role of sncRNAs, this newly identified sncRNA signature is likely to reflect the events leading to endometrial cancer, which can be exploited to dissect the carcinogenic process including novel biomarkers for early and non-invasive diagnosis of these tumors. Impact Journals LLC 2015-02-28 /pmc/articles/PMC4467107/ /pubmed/25686835 Text en Copyright: © 2015 Ravo et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Ravo, Maria
Cordella, Angela
Rinaldi, Antonio
Bruno, Giuseppina
Alexandrova, Elena
Saggese, Pasquale
Nassa, Giovanni
Giurato, Giorgio
Tarallo, Roberta
Marchese, Giovanna
Rizzo, Francesca
Stellato, Claudia
Biancardi, Rossella
Troisi, Jacopo
Di Spiezio Sardo, Attilio
Zullo, Fulvio
Weisz, Alessandro
Guida, Maurizio
Small non-coding RNA deregulation in endometrial carcinogenesis
title Small non-coding RNA deregulation in endometrial carcinogenesis
title_full Small non-coding RNA deregulation in endometrial carcinogenesis
title_fullStr Small non-coding RNA deregulation in endometrial carcinogenesis
title_full_unstemmed Small non-coding RNA deregulation in endometrial carcinogenesis
title_short Small non-coding RNA deregulation in endometrial carcinogenesis
title_sort small non-coding rna deregulation in endometrial carcinogenesis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467107/
https://www.ncbi.nlm.nih.gov/pubmed/25686835
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