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Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling
O-glycosylation is a common protein modification. Aberrant O-glycosylation is associated with many cancers. GALNT1 is a GalNAc-transferase that initiates protein O-glycosylation. We found that GALNT1 is frequently up-regulated in hepatocellular carcinoma (HCC) and is associated with poor patient sur...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467392/ https://www.ncbi.nlm.nih.gov/pubmed/25730904 |
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author | Huang, Miao-Juei Hu, Rey-Heng Chou, Chih-Hsing Hsu, Chia-Lang Liu, Ya-Wen Huang, John Hung, Ji-Shiang Lai, I-Rue Juan, Hsueh-Fen Yu, Sung-Liang Wu, Yao-Ming Huang, Min-Chuan |
author_facet | Huang, Miao-Juei Hu, Rey-Heng Chou, Chih-Hsing Hsu, Chia-Lang Liu, Ya-Wen Huang, John Hung, Ji-Shiang Lai, I-Rue Juan, Hsueh-Fen Yu, Sung-Liang Wu, Yao-Ming Huang, Min-Chuan |
author_sort | Huang, Miao-Juei |
collection | PubMed |
description | O-glycosylation is a common protein modification. Aberrant O-glycosylation is associated with many cancers. GALNT1 is a GalNAc-transferase that initiates protein O-glycosylation. We found that GALNT1 is frequently up-regulated in hepatocellular carcinoma (HCC) and is associated with poor patient survival. Overexpression of GALNT1 increased and knockdown decreased HCC cell migration and invasion. Knockdown of GALNT1 inhibited EGF-induced migration and invasion. Knockdown of GALNT1 decreased EGFR activation and increased EGFR degradation, by decreasing EGFR O-glycosylation. This study demonstrates that down-regulation of GALNT1 is sufficient to suppress malignant phenotype of HCC cells by decreasing EGFR signaling. Thus, GALNT1 is a potential target in HCC. |
format | Online Article Text |
id | pubmed-4467392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-44673922015-06-22 Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling Huang, Miao-Juei Hu, Rey-Heng Chou, Chih-Hsing Hsu, Chia-Lang Liu, Ya-Wen Huang, John Hung, Ji-Shiang Lai, I-Rue Juan, Hsueh-Fen Yu, Sung-Liang Wu, Yao-Ming Huang, Min-Chuan Oncotarget Research Paper O-glycosylation is a common protein modification. Aberrant O-glycosylation is associated with many cancers. GALNT1 is a GalNAc-transferase that initiates protein O-glycosylation. We found that GALNT1 is frequently up-regulated in hepatocellular carcinoma (HCC) and is associated with poor patient survival. Overexpression of GALNT1 increased and knockdown decreased HCC cell migration and invasion. Knockdown of GALNT1 inhibited EGF-induced migration and invasion. Knockdown of GALNT1 decreased EGFR activation and increased EGFR degradation, by decreasing EGFR O-glycosylation. This study demonstrates that down-regulation of GALNT1 is sufficient to suppress malignant phenotype of HCC cells by decreasing EGFR signaling. Thus, GALNT1 is a potential target in HCC. Impact Journals LLC 2015-02-04 /pmc/articles/PMC4467392/ /pubmed/25730904 Text en Copyright: © 2015 Huang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Huang, Miao-Juei Hu, Rey-Heng Chou, Chih-Hsing Hsu, Chia-Lang Liu, Ya-Wen Huang, John Hung, Ji-Shiang Lai, I-Rue Juan, Hsueh-Fen Yu, Sung-Liang Wu, Yao-Ming Huang, Min-Chuan Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling |
title | Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling |
title_full | Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling |
title_fullStr | Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling |
title_full_unstemmed | Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling |
title_short | Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling |
title_sort | knockdown of galnt1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing egfr signaling |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467392/ https://www.ncbi.nlm.nih.gov/pubmed/25730904 |
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