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Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling

O-glycosylation is a common protein modification. Aberrant O-glycosylation is associated with many cancers. GALNT1 is a GalNAc-transferase that initiates protein O-glycosylation. We found that GALNT1 is frequently up-regulated in hepatocellular carcinoma (HCC) and is associated with poor patient sur...

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Autores principales: Huang, Miao-Juei, Hu, Rey-Heng, Chou, Chih-Hsing, Hsu, Chia-Lang, Liu, Ya-Wen, Huang, John, Hung, Ji-Shiang, Lai, I-Rue, Juan, Hsueh-Fen, Yu, Sung-Liang, Wu, Yao-Ming, Huang, Min-Chuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467392/
https://www.ncbi.nlm.nih.gov/pubmed/25730904
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author Huang, Miao-Juei
Hu, Rey-Heng
Chou, Chih-Hsing
Hsu, Chia-Lang
Liu, Ya-Wen
Huang, John
Hung, Ji-Shiang
Lai, I-Rue
Juan, Hsueh-Fen
Yu, Sung-Liang
Wu, Yao-Ming
Huang, Min-Chuan
author_facet Huang, Miao-Juei
Hu, Rey-Heng
Chou, Chih-Hsing
Hsu, Chia-Lang
Liu, Ya-Wen
Huang, John
Hung, Ji-Shiang
Lai, I-Rue
Juan, Hsueh-Fen
Yu, Sung-Liang
Wu, Yao-Ming
Huang, Min-Chuan
author_sort Huang, Miao-Juei
collection PubMed
description O-glycosylation is a common protein modification. Aberrant O-glycosylation is associated with many cancers. GALNT1 is a GalNAc-transferase that initiates protein O-glycosylation. We found that GALNT1 is frequently up-regulated in hepatocellular carcinoma (HCC) and is associated with poor patient survival. Overexpression of GALNT1 increased and knockdown decreased HCC cell migration and invasion. Knockdown of GALNT1 inhibited EGF-induced migration and invasion. Knockdown of GALNT1 decreased EGFR activation and increased EGFR degradation, by decreasing EGFR O-glycosylation. This study demonstrates that down-regulation of GALNT1 is sufficient to suppress malignant phenotype of HCC cells by decreasing EGFR signaling. Thus, GALNT1 is a potential target in HCC.
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spelling pubmed-44673922015-06-22 Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling Huang, Miao-Juei Hu, Rey-Heng Chou, Chih-Hsing Hsu, Chia-Lang Liu, Ya-Wen Huang, John Hung, Ji-Shiang Lai, I-Rue Juan, Hsueh-Fen Yu, Sung-Liang Wu, Yao-Ming Huang, Min-Chuan Oncotarget Research Paper O-glycosylation is a common protein modification. Aberrant O-glycosylation is associated with many cancers. GALNT1 is a GalNAc-transferase that initiates protein O-glycosylation. We found that GALNT1 is frequently up-regulated in hepatocellular carcinoma (HCC) and is associated with poor patient survival. Overexpression of GALNT1 increased and knockdown decreased HCC cell migration and invasion. Knockdown of GALNT1 inhibited EGF-induced migration and invasion. Knockdown of GALNT1 decreased EGFR activation and increased EGFR degradation, by decreasing EGFR O-glycosylation. This study demonstrates that down-regulation of GALNT1 is sufficient to suppress malignant phenotype of HCC cells by decreasing EGFR signaling. Thus, GALNT1 is a potential target in HCC. Impact Journals LLC 2015-02-04 /pmc/articles/PMC4467392/ /pubmed/25730904 Text en Copyright: © 2015 Huang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Huang, Miao-Juei
Hu, Rey-Heng
Chou, Chih-Hsing
Hsu, Chia-Lang
Liu, Ya-Wen
Huang, John
Hung, Ji-Shiang
Lai, I-Rue
Juan, Hsueh-Fen
Yu, Sung-Liang
Wu, Yao-Ming
Huang, Min-Chuan
Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling
title Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling
title_full Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling
title_fullStr Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling
title_full_unstemmed Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling
title_short Knockdown of GALNT1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing EGFR signaling
title_sort knockdown of galnt1 suppresses malignant phenotype of hepatocellular carcinoma by suppressing egfr signaling
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467392/
https://www.ncbi.nlm.nih.gov/pubmed/25730904
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