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CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid
Glioblastomas respond differently to all-trans retinoic acid (RA) for unknown reasons. Because CRABP-II and FABP5 mediate RA intracellular signaling respectively and lead to distinct biological consequences, their expression patterns in different grades of astrocytomas and the glioblastoma cells lin...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467409/ https://www.ncbi.nlm.nih.gov/pubmed/25797252 |
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author | Xia, Shi-Lin Wu, Mo-Li Li, Hong Wang, Jia-Hui Chen, Nan-Nan Chen, Xiao-Yan Kong, Qing-You Sun, Zheng Liu, Jia |
author_facet | Xia, Shi-Lin Wu, Mo-Li Li, Hong Wang, Jia-Hui Chen, Nan-Nan Chen, Xiao-Yan Kong, Qing-You Sun, Zheng Liu, Jia |
author_sort | Xia, Shi-Lin |
collection | PubMed |
description | Glioblastomas respond differently to all-trans retinoic acid (RA) for unknown reasons. Because CRABP-II and FABP5 mediate RA intracellular signaling respectively and lead to distinct biological consequences, their expression patterns in different grades of astrocytomas and the glioblastoma cells lines LN18, LN428 and U251 were examined to identify potential correlations with RA sensitivities. The response of glioblastoma cells to RA, decitabine or the FABP5 competitive inhibitor, BMS309403, was analyzed. CRABP-II and FABP5 were expressed to varying degrees by the 84-astrocytoma cases examined. Treatment of LN428, U251 and LN18 cells with RA failed to suppress their growth; however, U251 proliferation was inhibited by decitabine. The combination of decitabine and RA suppressed the growth of all three cell lines and induced significant apoptosis of LN428 and U251 cells. Both CRABP-II and FABP5 were transcribed in the three cell lines but FABP5 proteins were undetectable in U251 cells. The ratio of CRABP-II to FABP5 was not altered after RA, decitabine or RA and decitabine treatment and the resistance of cells to RA was not reversed by BMS309403 treatment. In conclusion, CRABP-II and FABP5 expression patterns are neither related to the tumor grades nor correlated with RA sensitivity. Additional molecular factors may be present that determines the sensitivity of glioblastoma cells to RA. Dicitabine may improve the sensitivity of glioblastoma cells to RA, however, its underlying mechanism and its in vivo feasibility need to be investigated. |
format | Online Article Text |
id | pubmed-4467409 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-44674092015-06-22 CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid Xia, Shi-Lin Wu, Mo-Li Li, Hong Wang, Jia-Hui Chen, Nan-Nan Chen, Xiao-Yan Kong, Qing-You Sun, Zheng Liu, Jia Oncotarget Research Paper Glioblastomas respond differently to all-trans retinoic acid (RA) for unknown reasons. Because CRABP-II and FABP5 mediate RA intracellular signaling respectively and lead to distinct biological consequences, their expression patterns in different grades of astrocytomas and the glioblastoma cells lines LN18, LN428 and U251 were examined to identify potential correlations with RA sensitivities. The response of glioblastoma cells to RA, decitabine or the FABP5 competitive inhibitor, BMS309403, was analyzed. CRABP-II and FABP5 were expressed to varying degrees by the 84-astrocytoma cases examined. Treatment of LN428, U251 and LN18 cells with RA failed to suppress their growth; however, U251 proliferation was inhibited by decitabine. The combination of decitabine and RA suppressed the growth of all three cell lines and induced significant apoptosis of LN428 and U251 cells. Both CRABP-II and FABP5 were transcribed in the three cell lines but FABP5 proteins were undetectable in U251 cells. The ratio of CRABP-II to FABP5 was not altered after RA, decitabine or RA and decitabine treatment and the resistance of cells to RA was not reversed by BMS309403 treatment. In conclusion, CRABP-II and FABP5 expression patterns are neither related to the tumor grades nor correlated with RA sensitivity. Additional molecular factors may be present that determines the sensitivity of glioblastoma cells to RA. Dicitabine may improve the sensitivity of glioblastoma cells to RA, however, its underlying mechanism and its in vivo feasibility need to be investigated. Impact Journals LLC 2015-01-21 /pmc/articles/PMC4467409/ /pubmed/25797252 Text en Copyright: © 2015 Xia et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Xia, Shi-Lin Wu, Mo-Li Li, Hong Wang, Jia-Hui Chen, Nan-Nan Chen, Xiao-Yan Kong, Qing-You Sun, Zheng Liu, Jia CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid |
title | CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid |
title_full | CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid |
title_fullStr | CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid |
title_full_unstemmed | CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid |
title_short | CRABP-II- and FABP5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid |
title_sort | crabp-ii- and fabp5-independent responsiveness of human glioblastoma cells to all-trans retinoic acid |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467409/ https://www.ncbi.nlm.nih.gov/pubmed/25797252 |
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