miR-372 inhibits p62 in head and neck squamous cell carcinoma in vitro and in vivo

Here we showed that exogenous miR-372 expression and knockdown of p62 (sequestosome1 or SQSTM1), both increased migration of head and neck squamous cell carcinoma (HNSCC) cells. p62 induced phase II detoxification enzyme NADPH quinone oxidoreductase 1 (NQO1), which decreased ROS levels and cell migr...

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Autores principales: Yeh, Li-Yin, Liu, Chung-Ji, Wong, Yong-Kie, Chang, Christine, Lin, Shu-Chun, Chang, Kuo-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467422/
https://www.ncbi.nlm.nih.gov/pubmed/25714028
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author Yeh, Li-Yin
Liu, Chung-Ji
Wong, Yong-Kie
Chang, Christine
Lin, Shu-Chun
Chang, Kuo-Wei
author_facet Yeh, Li-Yin
Liu, Chung-Ji
Wong, Yong-Kie
Chang, Christine
Lin, Shu-Chun
Chang, Kuo-Wei
author_sort Yeh, Li-Yin
collection PubMed
description Here we showed that exogenous miR-372 expression and knockdown of p62 (sequestosome1 or SQSTM1), both increased migration of head and neck squamous cell carcinoma (HNSCC) cells. p62 induced phase II detoxification enzyme NADPH quinone oxidoreductase 1 (NQO1), which decreased ROS levels and cell migration. Also, miR-372 decreased p62 during hypoxia, thus increasing cell migration. Levels of miR-372 and p62 inversely correlated in human HNSCC tissues. Plasma levels of miR-372 was associated with advanced tumor stage and patient mortality. Both plasma and salivary miR-372 levels were decreased after tumor resection. We conclude that miR-372 decreases p62, thus increasing ROS and motility in HNSCC cells.
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spelling pubmed-44674222015-06-22 miR-372 inhibits p62 in head and neck squamous cell carcinoma in vitro and in vivo Yeh, Li-Yin Liu, Chung-Ji Wong, Yong-Kie Chang, Christine Lin, Shu-Chun Chang, Kuo-Wei Oncotarget Research Paper Here we showed that exogenous miR-372 expression and knockdown of p62 (sequestosome1 or SQSTM1), both increased migration of head and neck squamous cell carcinoma (HNSCC) cells. p62 induced phase II detoxification enzyme NADPH quinone oxidoreductase 1 (NQO1), which decreased ROS levels and cell migration. Also, miR-372 decreased p62 during hypoxia, thus increasing cell migration. Levels of miR-372 and p62 inversely correlated in human HNSCC tissues. Plasma levels of miR-372 was associated with advanced tumor stage and patient mortality. Both plasma and salivary miR-372 levels were decreased after tumor resection. We conclude that miR-372 decreases p62, thus increasing ROS and motility in HNSCC cells. Impact Journals LLC 2015-01-21 /pmc/articles/PMC4467422/ /pubmed/25714028 Text en Copyright: © 2015 Yeh et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Yeh, Li-Yin
Liu, Chung-Ji
Wong, Yong-Kie
Chang, Christine
Lin, Shu-Chun
Chang, Kuo-Wei
miR-372 inhibits p62 in head and neck squamous cell carcinoma in vitro and in vivo
title miR-372 inhibits p62 in head and neck squamous cell carcinoma in vitro and in vivo
title_full miR-372 inhibits p62 in head and neck squamous cell carcinoma in vitro and in vivo
title_fullStr miR-372 inhibits p62 in head and neck squamous cell carcinoma in vitro and in vivo
title_full_unstemmed miR-372 inhibits p62 in head and neck squamous cell carcinoma in vitro and in vivo
title_short miR-372 inhibits p62 in head and neck squamous cell carcinoma in vitro and in vivo
title_sort mir-372 inhibits p62 in head and neck squamous cell carcinoma in vitro and in vivo
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467422/
https://www.ncbi.nlm.nih.gov/pubmed/25714028
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