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Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity

Natural products using plants have received considerable attention because of their potential to treat various diseases. Arrabidaea chica (Humb. & Bonpl.) B. Verlot is a native tropical American vine with healing properties employed in folk medicine for wound healing, inflammation, and gastroint...

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Autores principales: Servat-Medina, Leila, González-Gómez, Alvaro, Reyes-Ortega, Felisa, Sousa, Ilza Maria Oliveira, de Cássia Almeida Queiroz, Nubia, Zago, Patricia Maria Wiziack, Jorge, Michelle Pedrosa, Monteiro, Karin Maia, de Carvalho, João Ernesto, Román, Julio San, Foglio, Mary Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467739/
https://www.ncbi.nlm.nih.gov/pubmed/26089666
http://dx.doi.org/10.2147/IJN.S83705
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author Servat-Medina, Leila
González-Gómez, Alvaro
Reyes-Ortega, Felisa
Sousa, Ilza Maria Oliveira
de Cássia Almeida Queiroz, Nubia
Zago, Patricia Maria Wiziack
Jorge, Michelle Pedrosa
Monteiro, Karin Maia
de Carvalho, João Ernesto
Román, Julio San
Foglio, Mary Ann
author_facet Servat-Medina, Leila
González-Gómez, Alvaro
Reyes-Ortega, Felisa
Sousa, Ilza Maria Oliveira
de Cássia Almeida Queiroz, Nubia
Zago, Patricia Maria Wiziack
Jorge, Michelle Pedrosa
Monteiro, Karin Maia
de Carvalho, João Ernesto
Román, Julio San
Foglio, Mary Ann
author_sort Servat-Medina, Leila
collection PubMed
description Natural products using plants have received considerable attention because of their potential to treat various diseases. Arrabidaea chica (Humb. & Bonpl.) B. Verlot is a native tropical American vine with healing properties employed in folk medicine for wound healing, inflammation, and gastrointestinal colic. Applying nanotechnology to plant extracts has revealed an advantageous strategy for herbal drugs considering the numerous features that nanostructured systems offer, including solubility, bioavailability, and pharmacological activity enhancement. The present study reports the preparation and characterization of chitosan–sodium tripolyphosphate nanoparticles (NPs) charged with A. chica standardized extract (AcE). Particle size and zeta potential were measured using a Zetasizer Nano ZS. The NP morphological characteristics were observed using scanning electron microscopy. Our studies indicated that the chitosan/sodium tripolyphosphate mass ratio of 5 and volume ratio of 10 were found to be the best condition to achieve the lowest NP sizes, with an average hydrodynamic diameter of 150±13 nm and a zeta potential of +45±2 mV. Particle size decreased with AcE addition (60±10.2 nm), suggesting an interaction between the extract’s composition and polymers. The NP biocompatibility was evaluated using human skin fibroblasts. AcE-NP demonstrated capability of maintaining cell viability at the lowest concentrations tested, stimulating cell proliferation at higher concentrations. Antiulcerogenic activity of AcE-NP was also evaluated with an acute gastric ulcer experimental model induced by ethanol and indomethacin. NPs loaded with A. chica extract reduced the ulcerative lesion index using lower doses compared with the free extract, suggesting that extract encapsulation in chitosan NPs allowed for a dose reduction for a gastroprotective effect. The AcE encapsulation offers an approach for further application of the A. chica extract that could be considered a potential candidate for ulcer-healing pharmaceutical systems.
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spelling pubmed-44677392015-06-18 Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity Servat-Medina, Leila González-Gómez, Alvaro Reyes-Ortega, Felisa Sousa, Ilza Maria Oliveira de Cássia Almeida Queiroz, Nubia Zago, Patricia Maria Wiziack Jorge, Michelle Pedrosa Monteiro, Karin Maia de Carvalho, João Ernesto Román, Julio San Foglio, Mary Ann Int J Nanomedicine Original Research Natural products using plants have received considerable attention because of their potential to treat various diseases. Arrabidaea chica (Humb. & Bonpl.) B. Verlot is a native tropical American vine with healing properties employed in folk medicine for wound healing, inflammation, and gastrointestinal colic. Applying nanotechnology to plant extracts has revealed an advantageous strategy for herbal drugs considering the numerous features that nanostructured systems offer, including solubility, bioavailability, and pharmacological activity enhancement. The present study reports the preparation and characterization of chitosan–sodium tripolyphosphate nanoparticles (NPs) charged with A. chica standardized extract (AcE). Particle size and zeta potential were measured using a Zetasizer Nano ZS. The NP morphological characteristics were observed using scanning electron microscopy. Our studies indicated that the chitosan/sodium tripolyphosphate mass ratio of 5 and volume ratio of 10 were found to be the best condition to achieve the lowest NP sizes, with an average hydrodynamic diameter of 150±13 nm and a zeta potential of +45±2 mV. Particle size decreased with AcE addition (60±10.2 nm), suggesting an interaction between the extract’s composition and polymers. The NP biocompatibility was evaluated using human skin fibroblasts. AcE-NP demonstrated capability of maintaining cell viability at the lowest concentrations tested, stimulating cell proliferation at higher concentrations. Antiulcerogenic activity of AcE-NP was also evaluated with an acute gastric ulcer experimental model induced by ethanol and indomethacin. NPs loaded with A. chica extract reduced the ulcerative lesion index using lower doses compared with the free extract, suggesting that extract encapsulation in chitosan NPs allowed for a dose reduction for a gastroprotective effect. The AcE encapsulation offers an approach for further application of the A. chica extract that could be considered a potential candidate for ulcer-healing pharmaceutical systems. Dove Medical Press 2015-06-09 /pmc/articles/PMC4467739/ /pubmed/26089666 http://dx.doi.org/10.2147/IJN.S83705 Text en © 2015 Servat-Medina et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Servat-Medina, Leila
González-Gómez, Alvaro
Reyes-Ortega, Felisa
Sousa, Ilza Maria Oliveira
de Cássia Almeida Queiroz, Nubia
Zago, Patricia Maria Wiziack
Jorge, Michelle Pedrosa
Monteiro, Karin Maia
de Carvalho, João Ernesto
Román, Julio San
Foglio, Mary Ann
Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity
title Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity
title_full Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity
title_fullStr Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity
title_full_unstemmed Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity
title_short Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity
title_sort chitosan–tripolyphosphate nanoparticles as arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467739/
https://www.ncbi.nlm.nih.gov/pubmed/26089666
http://dx.doi.org/10.2147/IJN.S83705
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