Development and characterization of microsatellite markers for Veratrum maackii (Melanthiaceae)(1)

PREMISE OF THE STUDY: Microsatellite markers were developed in Veratrum maackii (Melanthiaceae) to assess the pattern of population genetic structures across the species’ distribution. METHODS AND RESULTS: Ten polymorphic loci were isolated from V. maackii using 454 shotgun pyrosequencing and the bi...

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Detalles Bibliográficos
Autores principales: Li, Yue, Iijima, Satoshi, Maki, Masayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4467762/
https://www.ncbi.nlm.nih.gov/pubmed/26082881
http://dx.doi.org/10.3732/apps.1500030
Descripción
Sumario:PREMISE OF THE STUDY: Microsatellite markers were developed in Veratrum maackii (Melanthiaceae) to assess the pattern of population genetic structures across the species’ distribution. METHODS AND RESULTS: Ten polymorphic loci were isolated from V. maackii using 454 shotgun pyrosequencing and the biotin capture method. The number of alleles per locus ranged from three to 19. The observed heterozygosity and expected heterozygosity ranged from 0.143 to 0.929 and 0.269 to 0.904, respectively. CONCLUSIONS: These newly developed microsatellite markers are useful for assessing the genetic diversity, population structure, and demographic history of V. maackii across its distribution range.

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