Cargando…
Repeatability of Foveal Measurements Using Spectralis Optical Coherence Tomography Segmentation Software
PURPOSE: To investigate repeatability and reproducibility of thickness of eight individual retinal layers at axial and lateral foveal locations, as well as foveal width, measured from Spectralis spectral domain optical coherence tomography (SD-OCT) scans using newly available retinal layer segmentat...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468112/ https://www.ncbi.nlm.nih.gov/pubmed/26076457 http://dx.doi.org/10.1371/journal.pone.0129005 |
_version_ | 1782376442004242432 |
---|---|
author | Ctori, Irene Huntjens, Byki |
author_facet | Ctori, Irene Huntjens, Byki |
author_sort | Ctori, Irene |
collection | PubMed |
description | PURPOSE: To investigate repeatability and reproducibility of thickness of eight individual retinal layers at axial and lateral foveal locations, as well as foveal width, measured from Spectralis spectral domain optical coherence tomography (SD-OCT) scans using newly available retinal layer segmentation software. METHODS: High-resolution SD-OCT scans were acquired for 40 eyes of 40 young healthy volunteers. Two scans were obtained in a single visit for each participant. Using new Spectralis segmentation software, two investigators independently obtained thickness of each of eight individual retinal layers at 0°, 2° and 5° eccentricities nasal and temporal to foveal centre, as well as foveal width measurements. Bland-Altman Coefficient of Repeatability (CoR) was calculated for inter-investigator and inter-scan agreement of all retinal measurements. Spearman's ρ indicated correlation of manually located central retinal thickness (RT(0)) with automated minimum foveal thickness (MFT) measurements. In addition, we investigated nasal-temporal symmetry of individual retinal layer thickness within the foveal pit. RESULTS: Inter-scan CoR values ranged from 3.1μm for axial retinal nerve fibre layer thickness to 15.0μm for the ganglion cell layer at 5° eccentricity. Mean foveal width was 2550μm ± 322μm with a CoR of 13μm for inter-investigator and 40μm for inter-scan agreement. Correlation of RT(0) and MFT was very good (ρ = 0.97, P < 0.0005). There were no significant differences in thickness of any individual retinal layers at 2° nasal compared to temporal to fovea (P > 0.05); however this symmetry could not be found at 5° eccentricity. CONCLUSIONS: We demonstrate excellent repeatability and reproducibility of each of eight individual retinal layer thickness measurements within the fovea as well as foveal width using Spectralis SD-OCT segmentation software in a young, healthy cohort. Thickness of all individual retinal layers were symmetrical at 2°, but not at 5° eccentricity away from the fovea. |
format | Online Article Text |
id | pubmed-4468112 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44681122015-06-25 Repeatability of Foveal Measurements Using Spectralis Optical Coherence Tomography Segmentation Software Ctori, Irene Huntjens, Byki PLoS One Research Article PURPOSE: To investigate repeatability and reproducibility of thickness of eight individual retinal layers at axial and lateral foveal locations, as well as foveal width, measured from Spectralis spectral domain optical coherence tomography (SD-OCT) scans using newly available retinal layer segmentation software. METHODS: High-resolution SD-OCT scans were acquired for 40 eyes of 40 young healthy volunteers. Two scans were obtained in a single visit for each participant. Using new Spectralis segmentation software, two investigators independently obtained thickness of each of eight individual retinal layers at 0°, 2° and 5° eccentricities nasal and temporal to foveal centre, as well as foveal width measurements. Bland-Altman Coefficient of Repeatability (CoR) was calculated for inter-investigator and inter-scan agreement of all retinal measurements. Spearman's ρ indicated correlation of manually located central retinal thickness (RT(0)) with automated minimum foveal thickness (MFT) measurements. In addition, we investigated nasal-temporal symmetry of individual retinal layer thickness within the foveal pit. RESULTS: Inter-scan CoR values ranged from 3.1μm for axial retinal nerve fibre layer thickness to 15.0μm for the ganglion cell layer at 5° eccentricity. Mean foveal width was 2550μm ± 322μm with a CoR of 13μm for inter-investigator and 40μm for inter-scan agreement. Correlation of RT(0) and MFT was very good (ρ = 0.97, P < 0.0005). There were no significant differences in thickness of any individual retinal layers at 2° nasal compared to temporal to fovea (P > 0.05); however this symmetry could not be found at 5° eccentricity. CONCLUSIONS: We demonstrate excellent repeatability and reproducibility of each of eight individual retinal layer thickness measurements within the fovea as well as foveal width using Spectralis SD-OCT segmentation software in a young, healthy cohort. Thickness of all individual retinal layers were symmetrical at 2°, but not at 5° eccentricity away from the fovea. Public Library of Science 2015-06-15 /pmc/articles/PMC4468112/ /pubmed/26076457 http://dx.doi.org/10.1371/journal.pone.0129005 Text en © 2015 Ctori, Huntjens http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ctori, Irene Huntjens, Byki Repeatability of Foveal Measurements Using Spectralis Optical Coherence Tomography Segmentation Software |
title | Repeatability of Foveal Measurements Using Spectralis Optical Coherence Tomography Segmentation Software |
title_full | Repeatability of Foveal Measurements Using Spectralis Optical Coherence Tomography Segmentation Software |
title_fullStr | Repeatability of Foveal Measurements Using Spectralis Optical Coherence Tomography Segmentation Software |
title_full_unstemmed | Repeatability of Foveal Measurements Using Spectralis Optical Coherence Tomography Segmentation Software |
title_short | Repeatability of Foveal Measurements Using Spectralis Optical Coherence Tomography Segmentation Software |
title_sort | repeatability of foveal measurements using spectralis optical coherence tomography segmentation software |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468112/ https://www.ncbi.nlm.nih.gov/pubmed/26076457 http://dx.doi.org/10.1371/journal.pone.0129005 |
work_keys_str_mv | AT ctoriirene repeatabilityoffovealmeasurementsusingspectralisopticalcoherencetomographysegmentationsoftware AT huntjensbyki repeatabilityoffovealmeasurementsusingspectralisopticalcoherencetomographysegmentationsoftware |