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MiR-148a, a microRNA upregulated in the WNT subgroup tumors, inhibits invasion and tumorigenic potential of medulloblastoma cells by targeting Neuropilin 1

Medulloblastoma, a common pediatric malignant brain tumor consists of four molecular subgroups viz. WNT, SHH, Group 3 and Group 4. MiR-148a is over-expressed in the WNT subgroup tumors, which have the lowest incidence of metastasis and excellent survival among all medulloblastomas. MiR-148a was expr...

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Autores principales: Yogi, Kedar, Sridhar, Epari, Goel, Naina, Jalali, Rakesh, Goel, Atul, Moiyadi, Aliasgar, Thorat, Rahul, Panwalkar, Pooja, Khire, Atul, Dasgupta, Archya, Shetty, Prakash, Shirsat, Neelam Vishwanath
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468320/
https://www.ncbi.nlm.nih.gov/pubmed/26097868
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author Yogi, Kedar
Sridhar, Epari
Goel, Naina
Jalali, Rakesh
Goel, Atul
Moiyadi, Aliasgar
Thorat, Rahul
Panwalkar, Pooja
Khire, Atul
Dasgupta, Archya
Shetty, Prakash
Shirsat, Neelam Vishwanath
author_facet Yogi, Kedar
Sridhar, Epari
Goel, Naina
Jalali, Rakesh
Goel, Atul
Moiyadi, Aliasgar
Thorat, Rahul
Panwalkar, Pooja
Khire, Atul
Dasgupta, Archya
Shetty, Prakash
Shirsat, Neelam Vishwanath
author_sort Yogi, Kedar
collection PubMed
description Medulloblastoma, a common pediatric malignant brain tumor consists of four molecular subgroups viz. WNT, SHH, Group 3 and Group 4. MiR-148a is over-expressed in the WNT subgroup tumors, which have the lowest incidence of metastasis and excellent survival among all medulloblastomas. MiR-148a was expressed either in a transient manner using a synthetic mimic or in a stable doxycycline inducible manner using a lentiviral vector in non-WNT medulloblastoma cell lines. Expression of miR-148a to levels comparable to that in the WNT subgroup tumors was found to inhibit proliferation, clonogenic potential, invasion potential and tumorigenicity of medulloblastoma cells. MiR-148a expression in medulloblastoma cells brought about reduction in the expression of NRP1, a novel miR-148a target. Restoration of NRP1 expression in medulloblastoma cells was found to rescue the reduction in the invasion potential and tumorigenicity brought about by miR-148a expression. NRP1 is known to play role in multiple signaling pathways that promote tumor growth, invasion and metastasis. NRP1 expression in medulloblastomas was found to be associated with poor survival, with little or no expression in majority of the WNT tumors. The tumor suppressive effect of miR-148a expression accompanied by the down-regulation of NRP1 makes miR-148a an attractive therapeutic agent for the treatment of medulloblastomas.
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spelling pubmed-44683202015-06-19 MiR-148a, a microRNA upregulated in the WNT subgroup tumors, inhibits invasion and tumorigenic potential of medulloblastoma cells by targeting Neuropilin 1 Yogi, Kedar Sridhar, Epari Goel, Naina Jalali, Rakesh Goel, Atul Moiyadi, Aliasgar Thorat, Rahul Panwalkar, Pooja Khire, Atul Dasgupta, Archya Shetty, Prakash Shirsat, Neelam Vishwanath Oncoscience Research Paper Medulloblastoma, a common pediatric malignant brain tumor consists of four molecular subgroups viz. WNT, SHH, Group 3 and Group 4. MiR-148a is over-expressed in the WNT subgroup tumors, which have the lowest incidence of metastasis and excellent survival among all medulloblastomas. MiR-148a was expressed either in a transient manner using a synthetic mimic or in a stable doxycycline inducible manner using a lentiviral vector in non-WNT medulloblastoma cell lines. Expression of miR-148a to levels comparable to that in the WNT subgroup tumors was found to inhibit proliferation, clonogenic potential, invasion potential and tumorigenicity of medulloblastoma cells. MiR-148a expression in medulloblastoma cells brought about reduction in the expression of NRP1, a novel miR-148a target. Restoration of NRP1 expression in medulloblastoma cells was found to rescue the reduction in the invasion potential and tumorigenicity brought about by miR-148a expression. NRP1 is known to play role in multiple signaling pathways that promote tumor growth, invasion and metastasis. NRP1 expression in medulloblastomas was found to be associated with poor survival, with little or no expression in majority of the WNT tumors. The tumor suppressive effect of miR-148a expression accompanied by the down-regulation of NRP1 makes miR-148a an attractive therapeutic agent for the treatment of medulloblastomas. Impact Journals LLC 2015-03-02 /pmc/articles/PMC4468320/ /pubmed/26097868 Text en Copyright: © 2015 Yogi et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Yogi, Kedar
Sridhar, Epari
Goel, Naina
Jalali, Rakesh
Goel, Atul
Moiyadi, Aliasgar
Thorat, Rahul
Panwalkar, Pooja
Khire, Atul
Dasgupta, Archya
Shetty, Prakash
Shirsat, Neelam Vishwanath
MiR-148a, a microRNA upregulated in the WNT subgroup tumors, inhibits invasion and tumorigenic potential of medulloblastoma cells by targeting Neuropilin 1
title MiR-148a, a microRNA upregulated in the WNT subgroup tumors, inhibits invasion and tumorigenic potential of medulloblastoma cells by targeting Neuropilin 1
title_full MiR-148a, a microRNA upregulated in the WNT subgroup tumors, inhibits invasion and tumorigenic potential of medulloblastoma cells by targeting Neuropilin 1
title_fullStr MiR-148a, a microRNA upregulated in the WNT subgroup tumors, inhibits invasion and tumorigenic potential of medulloblastoma cells by targeting Neuropilin 1
title_full_unstemmed MiR-148a, a microRNA upregulated in the WNT subgroup tumors, inhibits invasion and tumorigenic potential of medulloblastoma cells by targeting Neuropilin 1
title_short MiR-148a, a microRNA upregulated in the WNT subgroup tumors, inhibits invasion and tumorigenic potential of medulloblastoma cells by targeting Neuropilin 1
title_sort mir-148a, a microrna upregulated in the wnt subgroup tumors, inhibits invasion and tumorigenic potential of medulloblastoma cells by targeting neuropilin 1
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468320/
https://www.ncbi.nlm.nih.gov/pubmed/26097868
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