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Antiviral activity of silymarin against chikungunya virus
The mosquito-borne chikungunya virus (CHIKV) causes chikungunya fever, with clinical presentations such as severe back and small joint pain, and debilitating arthritis associated with crippling pains that persist for weeks and even years. Although there are several studies to evaluate the efficacy o...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468427/ https://www.ncbi.nlm.nih.gov/pubmed/26078201 http://dx.doi.org/10.1038/srep11421 |
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author | Lani, Rafidah Hassandarvish, Pouya Chiam, Chun Wei Moghaddam, Ehsan Chu, Justin Jang Hann Rausalu, Kai Merits, Andres Higgs, Stephen Vanlandingham, Dana Abu Bakar, Sazaly Zandi, Keivan |
author_facet | Lani, Rafidah Hassandarvish, Pouya Chiam, Chun Wei Moghaddam, Ehsan Chu, Justin Jang Hann Rausalu, Kai Merits, Andres Higgs, Stephen Vanlandingham, Dana Abu Bakar, Sazaly Zandi, Keivan |
author_sort | Lani, Rafidah |
collection | PubMed |
description | The mosquito-borne chikungunya virus (CHIKV) causes chikungunya fever, with clinical presentations such as severe back and small joint pain, and debilitating arthritis associated with crippling pains that persist for weeks and even years. Although there are several studies to evaluate the efficacy of drugs against CHIKV, the treatment for chikungunya fever is mainly symptom-based and no effective licensed vaccine or antiviral are available. Here, we investigated the antiviral activity of three types of flavonoids against CHIKV in vitro replication. Three compounds: silymarin, quercetin and kaempferol were evaluated for their in vitro antiviral activities against CHIKV using a CHIKV replicon cell line and clinical isolate of CHIKV of Central/East African genotype. A cytopathic effect inhibition assay was used to determine their activities on CHIKV viral replication and quantitative reverse transcription PCR was used to calculate virus yield. Antiviral activity of effective compound was further investigated by evaluation of CHIKV protein expression using western blotting for CHIKV nsP1, nsP3, and E2E1 proteins. Briefly, silymarin exhibited significant antiviral activity against CHIKV, reducing both CHIKV replication efficiency and down-regulating production of viral proteins involved in replication. This study may have important consequence for broaden the chance of getting the effective antiviral for CHIKV infection. |
format | Online Article Text |
id | pubmed-4468427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-44684272015-06-18 Antiviral activity of silymarin against chikungunya virus Lani, Rafidah Hassandarvish, Pouya Chiam, Chun Wei Moghaddam, Ehsan Chu, Justin Jang Hann Rausalu, Kai Merits, Andres Higgs, Stephen Vanlandingham, Dana Abu Bakar, Sazaly Zandi, Keivan Sci Rep Article The mosquito-borne chikungunya virus (CHIKV) causes chikungunya fever, with clinical presentations such as severe back and small joint pain, and debilitating arthritis associated with crippling pains that persist for weeks and even years. Although there are several studies to evaluate the efficacy of drugs against CHIKV, the treatment for chikungunya fever is mainly symptom-based and no effective licensed vaccine or antiviral are available. Here, we investigated the antiviral activity of three types of flavonoids against CHIKV in vitro replication. Three compounds: silymarin, quercetin and kaempferol were evaluated for their in vitro antiviral activities against CHIKV using a CHIKV replicon cell line and clinical isolate of CHIKV of Central/East African genotype. A cytopathic effect inhibition assay was used to determine their activities on CHIKV viral replication and quantitative reverse transcription PCR was used to calculate virus yield. Antiviral activity of effective compound was further investigated by evaluation of CHIKV protein expression using western blotting for CHIKV nsP1, nsP3, and E2E1 proteins. Briefly, silymarin exhibited significant antiviral activity against CHIKV, reducing both CHIKV replication efficiency and down-regulating production of viral proteins involved in replication. This study may have important consequence for broaden the chance of getting the effective antiviral for CHIKV infection. Nature Publishing Group 2015-06-16 /pmc/articles/PMC4468427/ /pubmed/26078201 http://dx.doi.org/10.1038/srep11421 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Lani, Rafidah Hassandarvish, Pouya Chiam, Chun Wei Moghaddam, Ehsan Chu, Justin Jang Hann Rausalu, Kai Merits, Andres Higgs, Stephen Vanlandingham, Dana Abu Bakar, Sazaly Zandi, Keivan Antiviral activity of silymarin against chikungunya virus |
title | Antiviral activity of silymarin against chikungunya virus |
title_full | Antiviral activity of silymarin against chikungunya virus |
title_fullStr | Antiviral activity of silymarin against chikungunya virus |
title_full_unstemmed | Antiviral activity of silymarin against chikungunya virus |
title_short | Antiviral activity of silymarin against chikungunya virus |
title_sort | antiviral activity of silymarin against chikungunya virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468427/ https://www.ncbi.nlm.nih.gov/pubmed/26078201 http://dx.doi.org/10.1038/srep11421 |
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