Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury

Apelin-13 inhibits neuronal apoptosis caused by hydrogen peroxide, yet apoptosis following cerebral ischemia-reperfusion injury has rarely been studied. In this study, Apelin-13 (0.1 μg/g) was injected into the lateral ventricle of middle cerebral artery occlusion model rats. TTC, TUNEL, and immunoh...

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Autores principales: Yan, Xiao-ge, Cheng, Bao-hua, Wang, Xin, Ding, Liang-cai, Liu, Hai-qing, Chen, Jing, Bai, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468768/
https://www.ncbi.nlm.nih.gov/pubmed/26109951
http://dx.doi.org/10.4103/1673-5374.157243
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author Yan, Xiao-ge
Cheng, Bao-hua
Wang, Xin
Ding, Liang-cai
Liu, Hai-qing
Chen, Jing
Bai, Bo
author_facet Yan, Xiao-ge
Cheng, Bao-hua
Wang, Xin
Ding, Liang-cai
Liu, Hai-qing
Chen, Jing
Bai, Bo
author_sort Yan, Xiao-ge
collection PubMed
description Apelin-13 inhibits neuronal apoptosis caused by hydrogen peroxide, yet apoptosis following cerebral ischemia-reperfusion injury has rarely been studied. In this study, Apelin-13 (0.1 μg/g) was injected into the lateral ventricle of middle cerebral artery occlusion model rats. TTC, TUNEL, and immunohistochemical staining showed that compared with the cerebral ischemia/reperfusion group, infarct volume and apoptotic cell number at the ischemic penumbra region were decreased in the Apelin-13 treatment group. Additionally, Apelin-13 treatment increased Bcl-2 immunoreactivity and decreased caspase-3 immunoreactivity. Our findings suggest that Apelin-13 is neuroprotective against cerebral ischemia/reperfusion injury through inhibition of neuronal apoptosis.
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spelling pubmed-44687682015-06-24 Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury Yan, Xiao-ge Cheng, Bao-hua Wang, Xin Ding, Liang-cai Liu, Hai-qing Chen, Jing Bai, Bo Neural Regen Res Research Article Apelin-13 inhibits neuronal apoptosis caused by hydrogen peroxide, yet apoptosis following cerebral ischemia-reperfusion injury has rarely been studied. In this study, Apelin-13 (0.1 μg/g) was injected into the lateral ventricle of middle cerebral artery occlusion model rats. TTC, TUNEL, and immunohistochemical staining showed that compared with the cerebral ischemia/reperfusion group, infarct volume and apoptotic cell number at the ischemic penumbra region were decreased in the Apelin-13 treatment group. Additionally, Apelin-13 treatment increased Bcl-2 immunoreactivity and decreased caspase-3 immunoreactivity. Our findings suggest that Apelin-13 is neuroprotective against cerebral ischemia/reperfusion injury through inhibition of neuronal apoptosis. Medknow Publications & Media Pvt Ltd 2015-05 /pmc/articles/PMC4468768/ /pubmed/26109951 http://dx.doi.org/10.4103/1673-5374.157243 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yan, Xiao-ge
Cheng, Bao-hua
Wang, Xin
Ding, Liang-cai
Liu, Hai-qing
Chen, Jing
Bai, Bo
Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury
title Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury
title_full Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury
title_fullStr Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury
title_full_unstemmed Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury
title_short Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury
title_sort lateral intracerebroventricular injection of apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468768/
https://www.ncbi.nlm.nih.gov/pubmed/26109951
http://dx.doi.org/10.4103/1673-5374.157243
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