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Clinical Potentials of Cardiomyocytes Derived from Patient-Specific Induced Pluripotent Stem Cells

The lack of appropriate human cardiomyocyte-based experimental platform has largely hindered the study of cardiac diseases and the development of therapeutic strategies. To date, somatic cells isolated from human subjects can be reprogramed into induced pluripotent stem cells (iPSCs) and subsequentl...

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Detalles Bibliográficos
Autores principales: Ng, Kwong-Man, Law, Cheuk-Yiu, Tse, Hung-Fat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470173/
https://www.ncbi.nlm.nih.gov/pubmed/26237594
http://dx.doi.org/10.3390/jcm3041105
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author Ng, Kwong-Man
Law, Cheuk-Yiu
Tse, Hung-Fat
author_facet Ng, Kwong-Man
Law, Cheuk-Yiu
Tse, Hung-Fat
author_sort Ng, Kwong-Man
collection PubMed
description The lack of appropriate human cardiomyocyte-based experimental platform has largely hindered the study of cardiac diseases and the development of therapeutic strategies. To date, somatic cells isolated from human subjects can be reprogramed into induced pluripotent stem cells (iPSCs) and subsequently differentiated into functional cardiomyocytes. This powerful reprogramming technology provides a novel in vitro human cell-based platform for the study of human hereditary cardiac disorders. The clinical potential of using iPSCs derived from patients with inherited cardiac disorders for therapeutic studies have been increasingly highlighted. In this review, the standard procedures for generating patient-specific iPSCs and the latest commonly used cardiac differentiation protocols will be outlined. Furthermore, the progress and limitations of current applications of iPSCs and iPSCs-derived cardiomyocytes in cell replacement therapy, disease modeling, drug-testing and toxicology studies will be discussed in detail.
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spelling pubmed-44701732015-07-28 Clinical Potentials of Cardiomyocytes Derived from Patient-Specific Induced Pluripotent Stem Cells Ng, Kwong-Man Law, Cheuk-Yiu Tse, Hung-Fat J Clin Med Review The lack of appropriate human cardiomyocyte-based experimental platform has largely hindered the study of cardiac diseases and the development of therapeutic strategies. To date, somatic cells isolated from human subjects can be reprogramed into induced pluripotent stem cells (iPSCs) and subsequently differentiated into functional cardiomyocytes. This powerful reprogramming technology provides a novel in vitro human cell-based platform for the study of human hereditary cardiac disorders. The clinical potential of using iPSCs derived from patients with inherited cardiac disorders for therapeutic studies have been increasingly highlighted. In this review, the standard procedures for generating patient-specific iPSCs and the latest commonly used cardiac differentiation protocols will be outlined. Furthermore, the progress and limitations of current applications of iPSCs and iPSCs-derived cardiomyocytes in cell replacement therapy, disease modeling, drug-testing and toxicology studies will be discussed in detail. MDPI 2014-10-15 /pmc/articles/PMC4470173/ /pubmed/26237594 http://dx.doi.org/10.3390/jcm3041105 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Ng, Kwong-Man
Law, Cheuk-Yiu
Tse, Hung-Fat
Clinical Potentials of Cardiomyocytes Derived from Patient-Specific Induced Pluripotent Stem Cells
title Clinical Potentials of Cardiomyocytes Derived from Patient-Specific Induced Pluripotent Stem Cells
title_full Clinical Potentials of Cardiomyocytes Derived from Patient-Specific Induced Pluripotent Stem Cells
title_fullStr Clinical Potentials of Cardiomyocytes Derived from Patient-Specific Induced Pluripotent Stem Cells
title_full_unstemmed Clinical Potentials of Cardiomyocytes Derived from Patient-Specific Induced Pluripotent Stem Cells
title_short Clinical Potentials of Cardiomyocytes Derived from Patient-Specific Induced Pluripotent Stem Cells
title_sort clinical potentials of cardiomyocytes derived from patient-specific induced pluripotent stem cells
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470173/
https://www.ncbi.nlm.nih.gov/pubmed/26237594
http://dx.doi.org/10.3390/jcm3041105
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