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Mechanism of regulation of ‘chromosome kissing’ induced by Fob1 and its physiological significance
Protein-mediated “chromosome kissing” between two DNA sites in trans (or in cis) is known to facilitate three-dimensional control of gene expression and DNA replication. However, the mechanisms of regulation of the long-range interactions are unknown. Here, we show that the replication terminator pr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470286/ https://www.ncbi.nlm.nih.gov/pubmed/26063576 http://dx.doi.org/10.1101/gad.260844.115 |
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author | Choudhury, Malay Zaman, Shamsu Jiang, James C. Jazwinski, S. Michal Bastia, Deepak |
author_facet | Choudhury, Malay Zaman, Shamsu Jiang, James C. Jazwinski, S. Michal Bastia, Deepak |
author_sort | Choudhury, Malay |
collection | PubMed |
description | Protein-mediated “chromosome kissing” between two DNA sites in trans (or in cis) is known to facilitate three-dimensional control of gene expression and DNA replication. However, the mechanisms of regulation of the long-range interactions are unknown. Here, we show that the replication terminator protein Fob1 of Saccharomyces cerevisiae promoted chromosome kissing that initiated rDNA recombination and controlled the replicative life span (RLS). Oligomerization of Fob1 caused synaptic (kissing) interactions between pairs of terminator (Ter) sites that initiated recombination in rDNA. Fob1 oligomerization and Ter–Ter kissing were regulated by intramolecular inhibitory interactions between the C-terminal domain (C-Fob1) and the N-terminal domain (N-Fob1). Phosphomimetic substitutions of specific residues of C-Fob1 counteracted the inhibitory interaction. A mutation in either N-Fob1 that blocked Fob1 oligomerization or C-Fob1 that blocked its phosphorylation antagonized chromosome kissing and recombination and enhanced the RLS. The results provide novel insights into a mechanism of regulation of Fob1-mediated chromosome kissing. |
format | Online Article Text |
id | pubmed-4470286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-44702862015-12-01 Mechanism of regulation of ‘chromosome kissing’ induced by Fob1 and its physiological significance Choudhury, Malay Zaman, Shamsu Jiang, James C. Jazwinski, S. Michal Bastia, Deepak Genes Dev Research Paper Protein-mediated “chromosome kissing” between two DNA sites in trans (or in cis) is known to facilitate three-dimensional control of gene expression and DNA replication. However, the mechanisms of regulation of the long-range interactions are unknown. Here, we show that the replication terminator protein Fob1 of Saccharomyces cerevisiae promoted chromosome kissing that initiated rDNA recombination and controlled the replicative life span (RLS). Oligomerization of Fob1 caused synaptic (kissing) interactions between pairs of terminator (Ter) sites that initiated recombination in rDNA. Fob1 oligomerization and Ter–Ter kissing were regulated by intramolecular inhibitory interactions between the C-terminal domain (C-Fob1) and the N-terminal domain (N-Fob1). Phosphomimetic substitutions of specific residues of C-Fob1 counteracted the inhibitory interaction. A mutation in either N-Fob1 that blocked Fob1 oligomerization or C-Fob1 that blocked its phosphorylation antagonized chromosome kissing and recombination and enhanced the RLS. The results provide novel insights into a mechanism of regulation of Fob1-mediated chromosome kissing. Cold Spring Harbor Laboratory Press 2015-06-01 /pmc/articles/PMC4470286/ /pubmed/26063576 http://dx.doi.org/10.1101/gad.260844.115 Text en © 2015 Choudhury et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Paper Choudhury, Malay Zaman, Shamsu Jiang, James C. Jazwinski, S. Michal Bastia, Deepak Mechanism of regulation of ‘chromosome kissing’ induced by Fob1 and its physiological significance |
title | Mechanism of regulation of ‘chromosome kissing’ induced by Fob1 and its physiological significance |
title_full | Mechanism of regulation of ‘chromosome kissing’ induced by Fob1 and its physiological significance |
title_fullStr | Mechanism of regulation of ‘chromosome kissing’ induced by Fob1 and its physiological significance |
title_full_unstemmed | Mechanism of regulation of ‘chromosome kissing’ induced by Fob1 and its physiological significance |
title_short | Mechanism of regulation of ‘chromosome kissing’ induced by Fob1 and its physiological significance |
title_sort | mechanism of regulation of ‘chromosome kissing’ induced by fob1 and its physiological significance |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470286/ https://www.ncbi.nlm.nih.gov/pubmed/26063576 http://dx.doi.org/10.1101/gad.260844.115 |
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