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Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase

Hormesis is an adaptive response to a variety of oxidative stresses that renders cells resistant to harmful doses of stressing agents. Caffeic acid (CaA) is an important antioxidant that has protective effects against DNA damage caused by reactive oxygen species (ROS). However, whether CaA-induced p...

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Autores principales: Li, Y., Chen, L.J., Jiang, F., Yang, Y., Wang, X.X., Zhang, Z., Li, Z., Li, L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470308/
https://www.ncbi.nlm.nih.gov/pubmed/25831202
http://dx.doi.org/10.1590/1414-431X20143729
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author Li, Y.
Chen, L.J.
Jiang, F.
Yang, Y.
Wang, X.X.
Zhang, Z.
Li, Z.
Li, L.
author_facet Li, Y.
Chen, L.J.
Jiang, F.
Yang, Y.
Wang, X.X.
Zhang, Z.
Li, Z.
Li, L.
author_sort Li, Y.
collection PubMed
description Hormesis is an adaptive response to a variety of oxidative stresses that renders cells resistant to harmful doses of stressing agents. Caffeic acid (CaA) is an important antioxidant that has protective effects against DNA damage caused by reactive oxygen species (ROS). However, whether CaA-induced protection is a hormetic effect remains unknown, as is the molecular mechanism that is involved. We found that a low concentration (10 μM) of CaA increased human liver L-02 cell viability, attenuated hydrogen peroxide (H(2)O(2))-mediated decreases in cell viability, and decreased the extent of H(2)O(2)-induced DNA double-strand breaks (DSBs). In L-02 cells exposed to H(2)O(2), CaA treatment reduced ROS levels, which might have played a protective role. CaA also activated the extracellular signal-regulated kinase (ERK) signal pathway in a time-dependent manner. Inhibition of ERK by its inhibitor U0126 or by its specific small interfering RNA (siRNA) blocked the CaA-induced improvement in cell viability and the protective effects against H(2)O(2)-mediated DNA damage. This study adds to the understanding of the antioxidant effects of CaA by identifying a novel molecular mechanism of enhanced cell viability and protection against DNA damage.
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spelling pubmed-44703082015-07-02 Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase Li, Y. Chen, L.J. Jiang, F. Yang, Y. Wang, X.X. Zhang, Z. Li, Z. Li, L. Braz J Med Biol Res Biomedical Sciences Hormesis is an adaptive response to a variety of oxidative stresses that renders cells resistant to harmful doses of stressing agents. Caffeic acid (CaA) is an important antioxidant that has protective effects against DNA damage caused by reactive oxygen species (ROS). However, whether CaA-induced protection is a hormetic effect remains unknown, as is the molecular mechanism that is involved. We found that a low concentration (10 μM) of CaA increased human liver L-02 cell viability, attenuated hydrogen peroxide (H(2)O(2))-mediated decreases in cell viability, and decreased the extent of H(2)O(2)-induced DNA double-strand breaks (DSBs). In L-02 cells exposed to H(2)O(2), CaA treatment reduced ROS levels, which might have played a protective role. CaA also activated the extracellular signal-regulated kinase (ERK) signal pathway in a time-dependent manner. Inhibition of ERK by its inhibitor U0126 or by its specific small interfering RNA (siRNA) blocked the CaA-induced improvement in cell viability and the protective effects against H(2)O(2)-mediated DNA damage. This study adds to the understanding of the antioxidant effects of CaA by identifying a novel molecular mechanism of enhanced cell viability and protection against DNA damage. Associação Brasileira de Divulgação Científica 2015-03-27 /pmc/articles/PMC4470308/ /pubmed/25831202 http://dx.doi.org/10.1590/1414-431X20143729 Text en http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biomedical Sciences
Li, Y.
Chen, L.J.
Jiang, F.
Yang, Y.
Wang, X.X.
Zhang, Z.
Li, Z.
Li, L.
Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase
title Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase
title_full Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase
title_fullStr Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase
title_full_unstemmed Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase
title_short Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase
title_sort caffeic acid improves cell viability and protects against dna damage: involvement of reactive oxygen species and extracellular signal-regulated kinase
topic Biomedical Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470308/
https://www.ncbi.nlm.nih.gov/pubmed/25831202
http://dx.doi.org/10.1590/1414-431X20143729
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