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RNA-seq analysis of an apical meristem time series reveals a critical point in Arabidopsis thaliana flower initiation
BACKGROUND: Floral transition is a critical event in the life cycle of a flowering plant as it determines its reproductive success. Despite extensive studies of specific genes that regulate this process, the global changes in transcript expression profiles at the point when a vegetative meristem tra...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470339/ https://www.ncbi.nlm.nih.gov/pubmed/26084880 http://dx.doi.org/10.1186/s12864-015-1688-9 |
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author | Klepikova, Anna V. Logacheva, Maria D. Dmitriev, Sergey E. Penin, Aleksey A. |
author_facet | Klepikova, Anna V. Logacheva, Maria D. Dmitriev, Sergey E. Penin, Aleksey A. |
author_sort | Klepikova, Anna V. |
collection | PubMed |
description | BACKGROUND: Floral transition is a critical event in the life cycle of a flowering plant as it determines its reproductive success. Despite extensive studies of specific genes that regulate this process, the global changes in transcript expression profiles at the point when a vegetative meristem transitions into an inflorescence have not been reported. We analyzed gene expression during Arabidopsis thaliana meristem development under long day conditions from day 7 to 16 after germination in one-day increments. RESULTS: The dynamics of the expression of the main flowering regulators was consistent with previous reports: notably, the expression of FLOWERING LOCUS C (FLC) decreased over the course of the time series while expression of LEAFY (LFY) increased. This analysis revealed a developmental time point between 10 and 12 days after germination where FLC expression had decreased but LFY expression had not yet increased, which was characterized by a peak in the number of differentially expressed genes. Gene Ontology (GO) enrichment analysis of these genes identified an overrepresentation of genes related to the cell cycle. CONCLUSIONS: We discovered an unprecedented burst of differential expression of cell cycle related genes at one particular point during transition to flowering. We suggest that acceleration of rate of the divisions and partial cell cycling synchronization takes place at this point. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-1688-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4470339 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44703392015-06-18 RNA-seq analysis of an apical meristem time series reveals a critical point in Arabidopsis thaliana flower initiation Klepikova, Anna V. Logacheva, Maria D. Dmitriev, Sergey E. Penin, Aleksey A. BMC Genomics Research Article BACKGROUND: Floral transition is a critical event in the life cycle of a flowering plant as it determines its reproductive success. Despite extensive studies of specific genes that regulate this process, the global changes in transcript expression profiles at the point when a vegetative meristem transitions into an inflorescence have not been reported. We analyzed gene expression during Arabidopsis thaliana meristem development under long day conditions from day 7 to 16 after germination in one-day increments. RESULTS: The dynamics of the expression of the main flowering regulators was consistent with previous reports: notably, the expression of FLOWERING LOCUS C (FLC) decreased over the course of the time series while expression of LEAFY (LFY) increased. This analysis revealed a developmental time point between 10 and 12 days after germination where FLC expression had decreased but LFY expression had not yet increased, which was characterized by a peak in the number of differentially expressed genes. Gene Ontology (GO) enrichment analysis of these genes identified an overrepresentation of genes related to the cell cycle. CONCLUSIONS: We discovered an unprecedented burst of differential expression of cell cycle related genes at one particular point during transition to flowering. We suggest that acceleration of rate of the divisions and partial cell cycling synchronization takes place at this point. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-1688-9) contains supplementary material, which is available to authorized users. BioMed Central 2015-06-18 /pmc/articles/PMC4470339/ /pubmed/26084880 http://dx.doi.org/10.1186/s12864-015-1688-9 Text en © Klepikova et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Klepikova, Anna V. Logacheva, Maria D. Dmitriev, Sergey E. Penin, Aleksey A. RNA-seq analysis of an apical meristem time series reveals a critical point in Arabidopsis thaliana flower initiation |
title | RNA-seq analysis of an apical meristem time series reveals a critical point in Arabidopsis thaliana flower initiation |
title_full | RNA-seq analysis of an apical meristem time series reveals a critical point in Arabidopsis thaliana flower initiation |
title_fullStr | RNA-seq analysis of an apical meristem time series reveals a critical point in Arabidopsis thaliana flower initiation |
title_full_unstemmed | RNA-seq analysis of an apical meristem time series reveals a critical point in Arabidopsis thaliana flower initiation |
title_short | RNA-seq analysis of an apical meristem time series reveals a critical point in Arabidopsis thaliana flower initiation |
title_sort | rna-seq analysis of an apical meristem time series reveals a critical point in arabidopsis thaliana flower initiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470339/ https://www.ncbi.nlm.nih.gov/pubmed/26084880 http://dx.doi.org/10.1186/s12864-015-1688-9 |
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