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Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology
Bone tissue engineering is a powerful tool to treat bone defects caused by trauma, infection, tumors and other factors. Both silk fibroin (SF) and chitosan (CS) are non-toxic and have good biocompatibility, but are poor biological scaffolds when used alone. In this study, the microscopic structure a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470591/ https://www.ncbi.nlm.nih.gov/pubmed/26083846 http://dx.doi.org/10.1371/journal.pone.0128658 |
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author | Zeng, Shuguang Liu, Lei Shi, Yong Qiu, Junqi Fang, Wei Rong, Mingdeng Guo, Zehong Gao, Wenfeng |
author_facet | Zeng, Shuguang Liu, Lei Shi, Yong Qiu, Junqi Fang, Wei Rong, Mingdeng Guo, Zehong Gao, Wenfeng |
author_sort | Zeng, Shuguang |
collection | PubMed |
description | Bone tissue engineering is a powerful tool to treat bone defects caused by trauma, infection, tumors and other factors. Both silk fibroin (SF) and chitosan (CS) are non-toxic and have good biocompatibility, but are poor biological scaffolds when used alone. In this study, the microscopic structure and related properties of SF/CS composite scaffolds with different component ratios were examined. The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects. First, via freeze-drying and chemical crosslinking methods, SF/CS composites with different component ratios were prepared and their structure was characterized. Changes in the internal structure of the SF and CS mixture were observed, confirming that the mutual modification between the two components was complete and stable. The internal structure of the composite material was porous and three-dimensional with a porosity above 90%. We next studied the pore size, swelling ratio, water absorption ratio, degradation and in vitro cell proliferation. For the 40% SF-60% CS group, the pore size of the scaffold was suitable for the growth of osteoblasts, and the rate of degradation was steady. This favors the early adhesion, growth and proliferation of MG-63 cells. In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts. Thus, 40% SF-60% CS is a good material for bone tissue engineering. |
format | Online Article Text |
id | pubmed-4470591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44705912015-06-29 Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology Zeng, Shuguang Liu, Lei Shi, Yong Qiu, Junqi Fang, Wei Rong, Mingdeng Guo, Zehong Gao, Wenfeng PLoS One Research Article Bone tissue engineering is a powerful tool to treat bone defects caused by trauma, infection, tumors and other factors. Both silk fibroin (SF) and chitosan (CS) are non-toxic and have good biocompatibility, but are poor biological scaffolds when used alone. In this study, the microscopic structure and related properties of SF/CS composite scaffolds with different component ratios were examined. The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects. First, via freeze-drying and chemical crosslinking methods, SF/CS composites with different component ratios were prepared and their structure was characterized. Changes in the internal structure of the SF and CS mixture were observed, confirming that the mutual modification between the two components was complete and stable. The internal structure of the composite material was porous and three-dimensional with a porosity above 90%. We next studied the pore size, swelling ratio, water absorption ratio, degradation and in vitro cell proliferation. For the 40% SF-60% CS group, the pore size of the scaffold was suitable for the growth of osteoblasts, and the rate of degradation was steady. This favors the early adhesion, growth and proliferation of MG-63 cells. In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts. Thus, 40% SF-60% CS is a good material for bone tissue engineering. Public Library of Science 2015-06-17 /pmc/articles/PMC4470591/ /pubmed/26083846 http://dx.doi.org/10.1371/journal.pone.0128658 Text en © 2015 Zeng et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zeng, Shuguang Liu, Lei Shi, Yong Qiu, Junqi Fang, Wei Rong, Mingdeng Guo, Zehong Gao, Wenfeng Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology |
title | Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology |
title_full | Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology |
title_fullStr | Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology |
title_full_unstemmed | Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology |
title_short | Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology |
title_sort | characterization of silk fibroin/chitosan 3d porous scaffold and in vitro cytology |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470591/ https://www.ncbi.nlm.nih.gov/pubmed/26083846 http://dx.doi.org/10.1371/journal.pone.0128658 |
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