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Lipid peroxidation is essential for α‐synuclein‐induced cell death
Parkinson's disease is the second most common neurodegenerative disease and its pathogenesis is closely associated with oxidative stress. Deposition of aggregated α‐synuclein (α‐Syn) occurs in familial and sporadic forms of Parkinson's disease. Here, we studied the effect of oligomeric α‐S...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471127/ https://www.ncbi.nlm.nih.gov/pubmed/25580849 http://dx.doi.org/10.1111/jnc.13024 |
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author | Angelova, Plamena R. Horrocks, Mathew H. Klenerman, David Gandhi, Sonia Abramov, Andrey Y. Shchepinov, Mikhail S. |
author_facet | Angelova, Plamena R. Horrocks, Mathew H. Klenerman, David Gandhi, Sonia Abramov, Andrey Y. Shchepinov, Mikhail S. |
author_sort | Angelova, Plamena R. |
collection | PubMed |
description | Parkinson's disease is the second most common neurodegenerative disease and its pathogenesis is closely associated with oxidative stress. Deposition of aggregated α‐synuclein (α‐Syn) occurs in familial and sporadic forms of Parkinson's disease. Here, we studied the effect of oligomeric α‐Syn on one of the major markers of oxidative stress, lipid peroxidation, in primary co‐cultures of neurons and astrocytes. We found that oligomeric but not monomeric α‐Syn significantly increases the rate of production of reactive oxygen species, subsequently inducing lipid peroxidation in both neurons and astrocytes. Pre‐incubation of cells with isotope‐reinforced polyunsaturated fatty acids (D‐PUFAs) completely prevented the effect of oligomeric α‐Syn on lipid peroxidation. Inhibition of lipid peroxidation with D‐PUFAs further protected cells from cell death induced by oligomeric α‐Syn. Thus, lipid peroxidation induced by misfolding of α‐Syn may play an important role in the cellular mechanism of neuronal cell loss in Parkinson's disease. [Image: see text] We have found that aggregated α‐synuclein‐induced production of reactive oxygen species (ROS) that subsequently stimulates lipid peroxidation and cell death in neurons and astrocytes. Specific inhibition of lipid peroxidation by incubation with reinforced polyunsaturated fatty acids (D‐PUFAs) completely prevented the effect of α‐synuclein on lipid peroxidation and cell death. |
format | Online Article Text |
id | pubmed-4471127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-44711272015-06-18 Lipid peroxidation is essential for α‐synuclein‐induced cell death Angelova, Plamena R. Horrocks, Mathew H. Klenerman, David Gandhi, Sonia Abramov, Andrey Y. Shchepinov, Mikhail S. J Neurochem ORIGINAL ARTICLES Parkinson's disease is the second most common neurodegenerative disease and its pathogenesis is closely associated with oxidative stress. Deposition of aggregated α‐synuclein (α‐Syn) occurs in familial and sporadic forms of Parkinson's disease. Here, we studied the effect of oligomeric α‐Syn on one of the major markers of oxidative stress, lipid peroxidation, in primary co‐cultures of neurons and astrocytes. We found that oligomeric but not monomeric α‐Syn significantly increases the rate of production of reactive oxygen species, subsequently inducing lipid peroxidation in both neurons and astrocytes. Pre‐incubation of cells with isotope‐reinforced polyunsaturated fatty acids (D‐PUFAs) completely prevented the effect of oligomeric α‐Syn on lipid peroxidation. Inhibition of lipid peroxidation with D‐PUFAs further protected cells from cell death induced by oligomeric α‐Syn. Thus, lipid peroxidation induced by misfolding of α‐Syn may play an important role in the cellular mechanism of neuronal cell loss in Parkinson's disease. [Image: see text] We have found that aggregated α‐synuclein‐induced production of reactive oxygen species (ROS) that subsequently stimulates lipid peroxidation and cell death in neurons and astrocytes. Specific inhibition of lipid peroxidation by incubation with reinforced polyunsaturated fatty acids (D‐PUFAs) completely prevented the effect of α‐synuclein on lipid peroxidation and cell death. John Wiley and Sons Inc. 2015-03-01 2015-05 /pmc/articles/PMC4471127/ /pubmed/25580849 http://dx.doi.org/10.1111/jnc.13024 Text en © 2015 The Authors. Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of The International Society for Neurochemistry This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | ORIGINAL ARTICLES Angelova, Plamena R. Horrocks, Mathew H. Klenerman, David Gandhi, Sonia Abramov, Andrey Y. Shchepinov, Mikhail S. Lipid peroxidation is essential for α‐synuclein‐induced cell death |
title | Lipid peroxidation is essential for α‐synuclein‐induced cell death |
title_full | Lipid peroxidation is essential for α‐synuclein‐induced cell death |
title_fullStr | Lipid peroxidation is essential for α‐synuclein‐induced cell death |
title_full_unstemmed | Lipid peroxidation is essential for α‐synuclein‐induced cell death |
title_short | Lipid peroxidation is essential for α‐synuclein‐induced cell death |
title_sort | lipid peroxidation is essential for α‐synuclein‐induced cell death |
topic | ORIGINAL ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471127/ https://www.ncbi.nlm.nih.gov/pubmed/25580849 http://dx.doi.org/10.1111/jnc.13024 |
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