Cargando…

Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave

Despite the abundance of fossil remains for the extinct steppe bison (Bison priscus), an animal that was painted and engraved in numerous European Paleolithic caves, a complete mitochondrial genome sequence has never been obtained for this species. In the present study we collected bone samples from...

Descripción completa

Detalles Bibliográficos
Autores principales: Marsolier-Kergoat, Marie-Claude, Palacio, Pauline, Berthonaud, Véronique, Maksud, Frédéric, Stafford, Thomas, Bégouën, Robert, Elalouf, Jean-Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471230/
https://www.ncbi.nlm.nih.gov/pubmed/26083419
http://dx.doi.org/10.1371/journal.pone.0128267
_version_ 1782376873385263104
author Marsolier-Kergoat, Marie-Claude
Palacio, Pauline
Berthonaud, Véronique
Maksud, Frédéric
Stafford, Thomas
Bégouën, Robert
Elalouf, Jean-Marc
author_facet Marsolier-Kergoat, Marie-Claude
Palacio, Pauline
Berthonaud, Véronique
Maksud, Frédéric
Stafford, Thomas
Bégouën, Robert
Elalouf, Jean-Marc
author_sort Marsolier-Kergoat, Marie-Claude
collection PubMed
description Despite the abundance of fossil remains for the extinct steppe bison (Bison priscus), an animal that was painted and engraved in numerous European Paleolithic caves, a complete mitochondrial genome sequence has never been obtained for this species. In the present study we collected bone samples from a sector of the Trois-Frères Paleolithic cave (Ariège, France) that formerly functioned as a pitfall and was sealed before the end of the Pleistocene. Screening the DNA content of the samples collected from the ground surface revealed their contamination by Bos DNA. However, a 19,000-year-old rib collected on a rock apart the pathway delineated for modern visitors was devoid of such contaminants and reproducibly yielded Bison priscus DNA. High-throughput shotgun sequencing combined with conventional PCR analysis of the rib DNA extract enabled to reconstruct a complete mitochondrial genome sequence of 16,318 bp for the extinct steppe bison with a 10.4-fold coverage. Phylogenetic analyses robustly established the position of the Bison priscus mitochondrial genome as basal to the clade delineated by the genomes of the modern American Bison bison. The extinct steppe bison sequence, which exhibits 93 specific polymorphisms as compared to the published Bison bison mitochondrial genomes, provides an additional resource for the study of Bovinae specimens. Moreover this study of ancient DNA delineates a new research pathway for the analysis of the Magdalenian Trois-Frères cave.
format Online
Article
Text
id pubmed-4471230
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-44712302015-06-29 Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave Marsolier-Kergoat, Marie-Claude Palacio, Pauline Berthonaud, Véronique Maksud, Frédéric Stafford, Thomas Bégouën, Robert Elalouf, Jean-Marc PLoS One Research Article Despite the abundance of fossil remains for the extinct steppe bison (Bison priscus), an animal that was painted and engraved in numerous European Paleolithic caves, a complete mitochondrial genome sequence has never been obtained for this species. In the present study we collected bone samples from a sector of the Trois-Frères Paleolithic cave (Ariège, France) that formerly functioned as a pitfall and was sealed before the end of the Pleistocene. Screening the DNA content of the samples collected from the ground surface revealed their contamination by Bos DNA. However, a 19,000-year-old rib collected on a rock apart the pathway delineated for modern visitors was devoid of such contaminants and reproducibly yielded Bison priscus DNA. High-throughput shotgun sequencing combined with conventional PCR analysis of the rib DNA extract enabled to reconstruct a complete mitochondrial genome sequence of 16,318 bp for the extinct steppe bison with a 10.4-fold coverage. Phylogenetic analyses robustly established the position of the Bison priscus mitochondrial genome as basal to the clade delineated by the genomes of the modern American Bison bison. The extinct steppe bison sequence, which exhibits 93 specific polymorphisms as compared to the published Bison bison mitochondrial genomes, provides an additional resource for the study of Bovinae specimens. Moreover this study of ancient DNA delineates a new research pathway for the analysis of the Magdalenian Trois-Frères cave. Public Library of Science 2015-06-17 /pmc/articles/PMC4471230/ /pubmed/26083419 http://dx.doi.org/10.1371/journal.pone.0128267 Text en © 2015 Marsolier-Kergoat et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Marsolier-Kergoat, Marie-Claude
Palacio, Pauline
Berthonaud, Véronique
Maksud, Frédéric
Stafford, Thomas
Bégouën, Robert
Elalouf, Jean-Marc
Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave
title Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave
title_full Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave
title_fullStr Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave
title_full_unstemmed Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave
title_short Hunting the Extinct Steppe Bison (Bison priscus) Mitochondrial Genome in the Trois-Frères Paleolithic Painted Cave
title_sort hunting the extinct steppe bison (bison priscus) mitochondrial genome in the trois-frères paleolithic painted cave
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471230/
https://www.ncbi.nlm.nih.gov/pubmed/26083419
http://dx.doi.org/10.1371/journal.pone.0128267
work_keys_str_mv AT marsolierkergoatmarieclaude huntingtheextinctsteppebisonbisonpriscusmitochondrialgenomeinthetroisfrerespaleolithicpaintedcave
AT palaciopauline huntingtheextinctsteppebisonbisonpriscusmitochondrialgenomeinthetroisfrerespaleolithicpaintedcave
AT berthonaudveronique huntingtheextinctsteppebisonbisonpriscusmitochondrialgenomeinthetroisfrerespaleolithicpaintedcave
AT maksudfrederic huntingtheextinctsteppebisonbisonpriscusmitochondrialgenomeinthetroisfrerespaleolithicpaintedcave
AT staffordthomas huntingtheextinctsteppebisonbisonpriscusmitochondrialgenomeinthetroisfrerespaleolithicpaintedcave
AT begouenrobert huntingtheextinctsteppebisonbisonpriscusmitochondrialgenomeinthetroisfrerespaleolithicpaintedcave
AT elaloufjeanmarc huntingtheextinctsteppebisonbisonpriscusmitochondrialgenomeinthetroisfrerespaleolithicpaintedcave