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Expression of Adiponectin Receptors on Peripheral Blood Leukocytes of Hypertensive Children Is Associated with the Severity of Hypertension

The aim of the study was to find out whether peripheral blood leukocyte adiponectin receptors 1 and 2 (AdipoR1, AdipoR2) protein expression patterns (flow cytometry) differ between the primary hypertension children (n = 57) and healthy controls (n = 19) and if their expression levels are related to...

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Autores principales: Gackowska, Lidia, Litwin, Mieczyslaw, Trojanek, Joanna, Eljaszewicz, Andrzej, Kubiszewska, Izabela, Niemirska, Anna, Wierzbicka, Aldona, Michalkiewicz, Jacek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471253/
https://www.ncbi.nlm.nih.gov/pubmed/26146630
http://dx.doi.org/10.1155/2015/742646
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author Gackowska, Lidia
Litwin, Mieczyslaw
Trojanek, Joanna
Eljaszewicz, Andrzej
Kubiszewska, Izabela
Niemirska, Anna
Wierzbicka, Aldona
Michalkiewicz, Jacek
author_facet Gackowska, Lidia
Litwin, Mieczyslaw
Trojanek, Joanna
Eljaszewicz, Andrzej
Kubiszewska, Izabela
Niemirska, Anna
Wierzbicka, Aldona
Michalkiewicz, Jacek
author_sort Gackowska, Lidia
collection PubMed
description The aim of the study was to find out whether peripheral blood leukocyte adiponectin receptors 1 and 2 (AdipoR1, AdipoR2) protein expression patterns (flow cytometry) differ between the primary hypertension children (n = 57) and healthy controls (n = 19) and if their expression levels are related to selected clinical parameters. The group of 26 patients [AdipoR(−)] showed lower and the group of 31 patients [AdipoR(+)] showed higher AdipoRs protein expression than the control and each other (P < 0.01 for neutrophils, P < 0.05 for monocytes). The AdipoR(+) leukocytes expressed higher AdipoR1 mRNA levels (RT-PCR) than AdipoR(−) ones and controls (P = 0.022 and P = 0.007, resp.). Despite greater BMI, the AdipoR(−) patients had unchanged serum adiponectin levels. In contrast, AdipoR(+) patients had lower serum adiponectin concentrations than the AdipoR(−) ones and controls (P < 0.001). The AdipoR(+) patients had higher blood pressure (P = 0.042) and greater carotid intima-media thickness (P = 0.017) than the AdipoR(−) ones. The stage of hypertension was associated with increased neutrophil but not monocyte AdipoR1 density (AdipoR1 MFI) (P < 0.05). Severe ambulatory hypertension was presented more often in AdipoR(+) patients than in AdipoR(−) ones (51.6% versus 26.9%, resp.; P < 0.01). In conclusion, neutrophil AdipoRs upregulation was associated with early stages of vascular injury, hypertension severity, and low serum levels of adiponectin.
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spelling pubmed-44712532015-07-05 Expression of Adiponectin Receptors on Peripheral Blood Leukocytes of Hypertensive Children Is Associated with the Severity of Hypertension Gackowska, Lidia Litwin, Mieczyslaw Trojanek, Joanna Eljaszewicz, Andrzej Kubiszewska, Izabela Niemirska, Anna Wierzbicka, Aldona Michalkiewicz, Jacek Biomed Res Int Research Article The aim of the study was to find out whether peripheral blood leukocyte adiponectin receptors 1 and 2 (AdipoR1, AdipoR2) protein expression patterns (flow cytometry) differ between the primary hypertension children (n = 57) and healthy controls (n = 19) and if their expression levels are related to selected clinical parameters. The group of 26 patients [AdipoR(−)] showed lower and the group of 31 patients [AdipoR(+)] showed higher AdipoRs protein expression than the control and each other (P < 0.01 for neutrophils, P < 0.05 for monocytes). The AdipoR(+) leukocytes expressed higher AdipoR1 mRNA levels (RT-PCR) than AdipoR(−) ones and controls (P = 0.022 and P = 0.007, resp.). Despite greater BMI, the AdipoR(−) patients had unchanged serum adiponectin levels. In contrast, AdipoR(+) patients had lower serum adiponectin concentrations than the AdipoR(−) ones and controls (P < 0.001). The AdipoR(+) patients had higher blood pressure (P = 0.042) and greater carotid intima-media thickness (P = 0.017) than the AdipoR(−) ones. The stage of hypertension was associated with increased neutrophil but not monocyte AdipoR1 density (AdipoR1 MFI) (P < 0.05). Severe ambulatory hypertension was presented more often in AdipoR(+) patients than in AdipoR(−) ones (51.6% versus 26.9%, resp.; P < 0.01). In conclusion, neutrophil AdipoRs upregulation was associated with early stages of vascular injury, hypertension severity, and low serum levels of adiponectin. Hindawi Publishing Corporation 2015 2015-06-04 /pmc/articles/PMC4471253/ /pubmed/26146630 http://dx.doi.org/10.1155/2015/742646 Text en Copyright © 2015 Lidia Gackowska et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gackowska, Lidia
Litwin, Mieczyslaw
Trojanek, Joanna
Eljaszewicz, Andrzej
Kubiszewska, Izabela
Niemirska, Anna
Wierzbicka, Aldona
Michalkiewicz, Jacek
Expression of Adiponectin Receptors on Peripheral Blood Leukocytes of Hypertensive Children Is Associated with the Severity of Hypertension
title Expression of Adiponectin Receptors on Peripheral Blood Leukocytes of Hypertensive Children Is Associated with the Severity of Hypertension
title_full Expression of Adiponectin Receptors on Peripheral Blood Leukocytes of Hypertensive Children Is Associated with the Severity of Hypertension
title_fullStr Expression of Adiponectin Receptors on Peripheral Blood Leukocytes of Hypertensive Children Is Associated with the Severity of Hypertension
title_full_unstemmed Expression of Adiponectin Receptors on Peripheral Blood Leukocytes of Hypertensive Children Is Associated with the Severity of Hypertension
title_short Expression of Adiponectin Receptors on Peripheral Blood Leukocytes of Hypertensive Children Is Associated with the Severity of Hypertension
title_sort expression of adiponectin receptors on peripheral blood leukocytes of hypertensive children is associated with the severity of hypertension
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471253/
https://www.ncbi.nlm.nih.gov/pubmed/26146630
http://dx.doi.org/10.1155/2015/742646
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