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Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia
Trefoil factor 3 (TFF3), also called intestinal trefoil factor or Itf, is a 59 amino acid peptide found as a homodimer predominantly along the gastrointestinal tract and in serum. TFF3 expression is elevated during gastrointestinal adenoma progression and has been shown to promote mucosal wound heal...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471263/ https://www.ncbi.nlm.nih.gov/pubmed/26083576 http://dx.doi.org/10.1371/journal.pone.0126924 |
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author | Ge, Hongfei Gardner, Jonitha Wu, Xiaosu Rulifson, Ingrid Wang, Jinghong Xiong, Yumei Ye, Jingjing Belouski, Edward Cao, Ping Tang, Jie Lee, Ki Jeong Coberly, Suzanne Wu, Xinle Gupte, Jamila Miao, Lynn Yang, Li Nguyen, Natalie Shan, Bei Yeh, Wen-Chen Véniant, Murielle M. Li, Yang Baribault, Helene |
author_facet | Ge, Hongfei Gardner, Jonitha Wu, Xiaosu Rulifson, Ingrid Wang, Jinghong Xiong, Yumei Ye, Jingjing Belouski, Edward Cao, Ping Tang, Jie Lee, Ki Jeong Coberly, Suzanne Wu, Xinle Gupte, Jamila Miao, Lynn Yang, Li Nguyen, Natalie Shan, Bei Yeh, Wen-Chen Véniant, Murielle M. Li, Yang Baribault, Helene |
author_sort | Ge, Hongfei |
collection | PubMed |
description | Trefoil factor 3 (TFF3), also called intestinal trefoil factor or Itf, is a 59 amino acid peptide found as a homodimer predominantly along the gastrointestinal tract and in serum. TFF3 expression is elevated during gastrointestinal adenoma progression and has been shown to promote mucosal wound healing. Here we show that in contrast to other trefoil factor family members, TFF1 and TFF2, TFF3 is highly expressed in mouse duodenum, jejunum and ileum and that its expression is regulated by food intake. Overexpression of TFF3 using a recombinant adeno-associated virus (AAV) vector, or daily administration of recombinant TFF3 protein in vivo improved glucose tolerance in a diet-induced obesity mouse model. Body weight, fasting insulin, triglyceride, cholesterol and leptin levels were not affected by TFF3 treatment. Induction of mucinous metaplasia was observed in mice with AAV-mediated TFF3 overexpression, however, no such adverse histological effect was seen after the administration of recombinant TFF3 protein. Altogether these results suggest that the therapeutic potential of targeting TFF3 to treat T2D may be limited. |
format | Online Article Text |
id | pubmed-4471263 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44712632015-06-29 Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia Ge, Hongfei Gardner, Jonitha Wu, Xiaosu Rulifson, Ingrid Wang, Jinghong Xiong, Yumei Ye, Jingjing Belouski, Edward Cao, Ping Tang, Jie Lee, Ki Jeong Coberly, Suzanne Wu, Xinle Gupte, Jamila Miao, Lynn Yang, Li Nguyen, Natalie Shan, Bei Yeh, Wen-Chen Véniant, Murielle M. Li, Yang Baribault, Helene PLoS One Research Article Trefoil factor 3 (TFF3), also called intestinal trefoil factor or Itf, is a 59 amino acid peptide found as a homodimer predominantly along the gastrointestinal tract and in serum. TFF3 expression is elevated during gastrointestinal adenoma progression and has been shown to promote mucosal wound healing. Here we show that in contrast to other trefoil factor family members, TFF1 and TFF2, TFF3 is highly expressed in mouse duodenum, jejunum and ileum and that its expression is regulated by food intake. Overexpression of TFF3 using a recombinant adeno-associated virus (AAV) vector, or daily administration of recombinant TFF3 protein in vivo improved glucose tolerance in a diet-induced obesity mouse model. Body weight, fasting insulin, triglyceride, cholesterol and leptin levels were not affected by TFF3 treatment. Induction of mucinous metaplasia was observed in mice with AAV-mediated TFF3 overexpression, however, no such adverse histological effect was seen after the administration of recombinant TFF3 protein. Altogether these results suggest that the therapeutic potential of targeting TFF3 to treat T2D may be limited. Public Library of Science 2015-06-17 /pmc/articles/PMC4471263/ /pubmed/26083576 http://dx.doi.org/10.1371/journal.pone.0126924 Text en © 2015 Ge et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ge, Hongfei Gardner, Jonitha Wu, Xiaosu Rulifson, Ingrid Wang, Jinghong Xiong, Yumei Ye, Jingjing Belouski, Edward Cao, Ping Tang, Jie Lee, Ki Jeong Coberly, Suzanne Wu, Xinle Gupte, Jamila Miao, Lynn Yang, Li Nguyen, Natalie Shan, Bei Yeh, Wen-Chen Véniant, Murielle M. Li, Yang Baribault, Helene Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia |
title | Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia |
title_full | Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia |
title_fullStr | Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia |
title_full_unstemmed | Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia |
title_short | Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia |
title_sort | trefoil factor 3 (tff3) is regulated by food intake, improves glucose tolerance and induces mucinous metaplasia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471263/ https://www.ncbi.nlm.nih.gov/pubmed/26083576 http://dx.doi.org/10.1371/journal.pone.0126924 |
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