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Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia

Trefoil factor 3 (TFF3), also called intestinal trefoil factor or Itf, is a 59 amino acid peptide found as a homodimer predominantly along the gastrointestinal tract and in serum. TFF3 expression is elevated during gastrointestinal adenoma progression and has been shown to promote mucosal wound heal...

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Autores principales: Ge, Hongfei, Gardner, Jonitha, Wu, Xiaosu, Rulifson, Ingrid, Wang, Jinghong, Xiong, Yumei, Ye, Jingjing, Belouski, Edward, Cao, Ping, Tang, Jie, Lee, Ki Jeong, Coberly, Suzanne, Wu, Xinle, Gupte, Jamila, Miao, Lynn, Yang, Li, Nguyen, Natalie, Shan, Bei, Yeh, Wen-Chen, Véniant, Murielle M., Li, Yang, Baribault, Helene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471263/
https://www.ncbi.nlm.nih.gov/pubmed/26083576
http://dx.doi.org/10.1371/journal.pone.0126924
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author Ge, Hongfei
Gardner, Jonitha
Wu, Xiaosu
Rulifson, Ingrid
Wang, Jinghong
Xiong, Yumei
Ye, Jingjing
Belouski, Edward
Cao, Ping
Tang, Jie
Lee, Ki Jeong
Coberly, Suzanne
Wu, Xinle
Gupte, Jamila
Miao, Lynn
Yang, Li
Nguyen, Natalie
Shan, Bei
Yeh, Wen-Chen
Véniant, Murielle M.
Li, Yang
Baribault, Helene
author_facet Ge, Hongfei
Gardner, Jonitha
Wu, Xiaosu
Rulifson, Ingrid
Wang, Jinghong
Xiong, Yumei
Ye, Jingjing
Belouski, Edward
Cao, Ping
Tang, Jie
Lee, Ki Jeong
Coberly, Suzanne
Wu, Xinle
Gupte, Jamila
Miao, Lynn
Yang, Li
Nguyen, Natalie
Shan, Bei
Yeh, Wen-Chen
Véniant, Murielle M.
Li, Yang
Baribault, Helene
author_sort Ge, Hongfei
collection PubMed
description Trefoil factor 3 (TFF3), also called intestinal trefoil factor or Itf, is a 59 amino acid peptide found as a homodimer predominantly along the gastrointestinal tract and in serum. TFF3 expression is elevated during gastrointestinal adenoma progression and has been shown to promote mucosal wound healing. Here we show that in contrast to other trefoil factor family members, TFF1 and TFF2, TFF3 is highly expressed in mouse duodenum, jejunum and ileum and that its expression is regulated by food intake. Overexpression of TFF3 using a recombinant adeno-associated virus (AAV) vector, or daily administration of recombinant TFF3 protein in vivo improved glucose tolerance in a diet-induced obesity mouse model. Body weight, fasting insulin, triglyceride, cholesterol and leptin levels were not affected by TFF3 treatment. Induction of mucinous metaplasia was observed in mice with AAV-mediated TFF3 overexpression, however, no such adverse histological effect was seen after the administration of recombinant TFF3 protein. Altogether these results suggest that the therapeutic potential of targeting TFF3 to treat T2D may be limited.
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spelling pubmed-44712632015-06-29 Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia Ge, Hongfei Gardner, Jonitha Wu, Xiaosu Rulifson, Ingrid Wang, Jinghong Xiong, Yumei Ye, Jingjing Belouski, Edward Cao, Ping Tang, Jie Lee, Ki Jeong Coberly, Suzanne Wu, Xinle Gupte, Jamila Miao, Lynn Yang, Li Nguyen, Natalie Shan, Bei Yeh, Wen-Chen Véniant, Murielle M. Li, Yang Baribault, Helene PLoS One Research Article Trefoil factor 3 (TFF3), also called intestinal trefoil factor or Itf, is a 59 amino acid peptide found as a homodimer predominantly along the gastrointestinal tract and in serum. TFF3 expression is elevated during gastrointestinal adenoma progression and has been shown to promote mucosal wound healing. Here we show that in contrast to other trefoil factor family members, TFF1 and TFF2, TFF3 is highly expressed in mouse duodenum, jejunum and ileum and that its expression is regulated by food intake. Overexpression of TFF3 using a recombinant adeno-associated virus (AAV) vector, or daily administration of recombinant TFF3 protein in vivo improved glucose tolerance in a diet-induced obesity mouse model. Body weight, fasting insulin, triglyceride, cholesterol and leptin levels were not affected by TFF3 treatment. Induction of mucinous metaplasia was observed in mice with AAV-mediated TFF3 overexpression, however, no such adverse histological effect was seen after the administration of recombinant TFF3 protein. Altogether these results suggest that the therapeutic potential of targeting TFF3 to treat T2D may be limited. Public Library of Science 2015-06-17 /pmc/articles/PMC4471263/ /pubmed/26083576 http://dx.doi.org/10.1371/journal.pone.0126924 Text en © 2015 Ge et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ge, Hongfei
Gardner, Jonitha
Wu, Xiaosu
Rulifson, Ingrid
Wang, Jinghong
Xiong, Yumei
Ye, Jingjing
Belouski, Edward
Cao, Ping
Tang, Jie
Lee, Ki Jeong
Coberly, Suzanne
Wu, Xinle
Gupte, Jamila
Miao, Lynn
Yang, Li
Nguyen, Natalie
Shan, Bei
Yeh, Wen-Chen
Véniant, Murielle M.
Li, Yang
Baribault, Helene
Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia
title Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia
title_full Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia
title_fullStr Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia
title_full_unstemmed Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia
title_short Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia
title_sort trefoil factor 3 (tff3) is regulated by food intake, improves glucose tolerance and induces mucinous metaplasia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471263/
https://www.ncbi.nlm.nih.gov/pubmed/26083576
http://dx.doi.org/10.1371/journal.pone.0126924
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