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Epoetin Alpha and Epoetin Zeta: A Comparative Study on Stimulation of Angiogenesis and Wound Repair in an Experimental Model of Burn Injury
Deep second-degree burns are characterized by delayed formation of granulation tissue and impaired angiogenesis. Erythropoietin (EPO) is able to stimulate angiogenesis and mitosis, activating vascularization and cell cycle. The aim of our study was to investigate whether two biosimilar recombinant h...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471383/ https://www.ncbi.nlm.nih.gov/pubmed/26146639 http://dx.doi.org/10.1155/2015/968927 |
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author | Irrera, Natasha Bitto, Alessandra Pizzino, Gabriele Vaccaro, Mario Squadrito, Francesco Galeano, Mariarosaria Stagno d'Alcontres, Francesco Stagno d'Alcontres, Ferdinando Buemi, Michele Minutoli, Letteria Colonna, Michele Rosario Altavilla, Domenica |
author_facet | Irrera, Natasha Bitto, Alessandra Pizzino, Gabriele Vaccaro, Mario Squadrito, Francesco Galeano, Mariarosaria Stagno d'Alcontres, Francesco Stagno d'Alcontres, Ferdinando Buemi, Michele Minutoli, Letteria Colonna, Michele Rosario Altavilla, Domenica |
author_sort | Irrera, Natasha |
collection | PubMed |
description | Deep second-degree burns are characterized by delayed formation of granulation tissue and impaired angiogenesis. Erythropoietin (EPO) is able to stimulate angiogenesis and mitosis, activating vascularization and cell cycle. The aim of our study was to investigate whether two biosimilar recombinant human erythropoietins, EPO-α and EPO-Z, may promote these processes in an experimental model of burn injury. A total of 84 mice were used and a scald burn was produced on the back after shaving, in 80°C water for 10 seconds. Mice were then randomized to receive EPO-α (400 units/kg/day/sc) or EPO-Z (400 units/kg/day/sc) or their vehicle (100 μL/day/sc 0.9% NaCl solution). After 12 days, both EPO-α and EPO-Z increased VEGF protein expression. EPO-α caused an increased cyclin D1/CDK6 and cyclin E/CDK2 expression compared with vehicle and EPO-Z (p<0.001). Our study showed that EPO-α and EPO-Z accelerated wound closure and angiogenesis; however EPO-α resulted more effectively in achieving complete skin regeneration. Our data suggest that EPO-α and EPO-Z are not biosimilars for the wound healing effects. The higher efficacy of EPO-α might be likely due to its different conformational structure leading to a more efficient cell proliferation and skin remodelling. |
format | Online Article Text |
id | pubmed-4471383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-44713832015-07-05 Epoetin Alpha and Epoetin Zeta: A Comparative Study on Stimulation of Angiogenesis and Wound Repair in an Experimental Model of Burn Injury Irrera, Natasha Bitto, Alessandra Pizzino, Gabriele Vaccaro, Mario Squadrito, Francesco Galeano, Mariarosaria Stagno d'Alcontres, Francesco Stagno d'Alcontres, Ferdinando Buemi, Michele Minutoli, Letteria Colonna, Michele Rosario Altavilla, Domenica Biomed Res Int Research Article Deep second-degree burns are characterized by delayed formation of granulation tissue and impaired angiogenesis. Erythropoietin (EPO) is able to stimulate angiogenesis and mitosis, activating vascularization and cell cycle. The aim of our study was to investigate whether two biosimilar recombinant human erythropoietins, EPO-α and EPO-Z, may promote these processes in an experimental model of burn injury. A total of 84 mice were used and a scald burn was produced on the back after shaving, in 80°C water for 10 seconds. Mice were then randomized to receive EPO-α (400 units/kg/day/sc) or EPO-Z (400 units/kg/day/sc) or their vehicle (100 μL/day/sc 0.9% NaCl solution). After 12 days, both EPO-α and EPO-Z increased VEGF protein expression. EPO-α caused an increased cyclin D1/CDK6 and cyclin E/CDK2 expression compared with vehicle and EPO-Z (p<0.001). Our study showed that EPO-α and EPO-Z accelerated wound closure and angiogenesis; however EPO-α resulted more effectively in achieving complete skin regeneration. Our data suggest that EPO-α and EPO-Z are not biosimilars for the wound healing effects. The higher efficacy of EPO-α might be likely due to its different conformational structure leading to a more efficient cell proliferation and skin remodelling. Hindawi Publishing Corporation 2015 2015-06-04 /pmc/articles/PMC4471383/ /pubmed/26146639 http://dx.doi.org/10.1155/2015/968927 Text en Copyright © 2015 Natasha Irrera et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Irrera, Natasha Bitto, Alessandra Pizzino, Gabriele Vaccaro, Mario Squadrito, Francesco Galeano, Mariarosaria Stagno d'Alcontres, Francesco Stagno d'Alcontres, Ferdinando Buemi, Michele Minutoli, Letteria Colonna, Michele Rosario Altavilla, Domenica Epoetin Alpha and Epoetin Zeta: A Comparative Study on Stimulation of Angiogenesis and Wound Repair in an Experimental Model of Burn Injury |
title | Epoetin Alpha and Epoetin Zeta: A Comparative Study on Stimulation of Angiogenesis and Wound Repair in an Experimental Model of Burn Injury |
title_full | Epoetin Alpha and Epoetin Zeta: A Comparative Study on Stimulation of Angiogenesis and Wound Repair in an Experimental Model of Burn Injury |
title_fullStr | Epoetin Alpha and Epoetin Zeta: A Comparative Study on Stimulation of Angiogenesis and Wound Repair in an Experimental Model of Burn Injury |
title_full_unstemmed | Epoetin Alpha and Epoetin Zeta: A Comparative Study on Stimulation of Angiogenesis and Wound Repair in an Experimental Model of Burn Injury |
title_short | Epoetin Alpha and Epoetin Zeta: A Comparative Study on Stimulation of Angiogenesis and Wound Repair in an Experimental Model of Burn Injury |
title_sort | epoetin alpha and epoetin zeta: a comparative study on stimulation of angiogenesis and wound repair in an experimental model of burn injury |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471383/ https://www.ncbi.nlm.nih.gov/pubmed/26146639 http://dx.doi.org/10.1155/2015/968927 |
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