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Fluorescence Microscopy Visualization of Contacts Between Objects**

The area of contact between two objects was detected by using the strong enhancement of the fluorescence of rigidochromic probe molecules attached to one of the surfaces. Confinement of the molecules suppresses nonradiative decay and turns on the fluorescence. The approach is demonstrated by imaging...

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Detalles Bibliográficos
Autores principales: Suhina, Tomislav, Weber, Bart, Carpentier, Chantal E, Lorincz, Kinga, Schall, Peter, Bonn, Daniel, Brouwer, Albert M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471612/
https://www.ncbi.nlm.nih.gov/pubmed/25631338
http://dx.doi.org/10.1002/anie.201410240
Descripción
Sumario:The area of contact between two objects was detected by using the strong enhancement of the fluorescence of rigidochromic probe molecules attached to one of the surfaces. Confinement of the molecules suppresses nonradiative decay and turns on the fluorescence. The approach is demonstrated by imaging of the contact area of a plastic sphere in contact with a flat glass surface. Our results agree excellently with the prediction of Hertz’s classical theory based on elastic deformation.