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Fluorescence Microscopy Visualization of Contacts Between Objects**
The area of contact between two objects was detected by using the strong enhancement of the fluorescence of rigidochromic probe molecules attached to one of the surfaces. Confinement of the molecules suppresses nonradiative decay and turns on the fluorescence. The approach is demonstrated by imaging...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
WILEY-VCH Verlag
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471612/ https://www.ncbi.nlm.nih.gov/pubmed/25631338 http://dx.doi.org/10.1002/anie.201410240 |
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author | Suhina, Tomislav Weber, Bart Carpentier, Chantal E Lorincz, Kinga Schall, Peter Bonn, Daniel Brouwer, Albert M |
author_facet | Suhina, Tomislav Weber, Bart Carpentier, Chantal E Lorincz, Kinga Schall, Peter Bonn, Daniel Brouwer, Albert M |
author_sort | Suhina, Tomislav |
collection | PubMed |
description | The area of contact between two objects was detected by using the strong enhancement of the fluorescence of rigidochromic probe molecules attached to one of the surfaces. Confinement of the molecules suppresses nonradiative decay and turns on the fluorescence. The approach is demonstrated by imaging of the contact area of a plastic sphere in contact with a flat glass surface. Our results agree excellently with the prediction of Hertz’s classical theory based on elastic deformation. |
format | Online Article Text |
id | pubmed-4471612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | WILEY-VCH Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-44716122015-06-23 Fluorescence Microscopy Visualization of Contacts Between Objects** Suhina, Tomislav Weber, Bart Carpentier, Chantal E Lorincz, Kinga Schall, Peter Bonn, Daniel Brouwer, Albert M Angew Chem Int Ed Engl Communications The area of contact between two objects was detected by using the strong enhancement of the fluorescence of rigidochromic probe molecules attached to one of the surfaces. Confinement of the molecules suppresses nonradiative decay and turns on the fluorescence. The approach is demonstrated by imaging of the contact area of a plastic sphere in contact with a flat glass surface. Our results agree excellently with the prediction of Hertz’s classical theory based on elastic deformation. WILEY-VCH Verlag 2015-03-16 2015-01-28 /pmc/articles/PMC4471612/ /pubmed/25631338 http://dx.doi.org/10.1002/anie.201410240 Text en © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution Non-Commercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Communications Suhina, Tomislav Weber, Bart Carpentier, Chantal E Lorincz, Kinga Schall, Peter Bonn, Daniel Brouwer, Albert M Fluorescence Microscopy Visualization of Contacts Between Objects** |
title | Fluorescence Microscopy Visualization of Contacts Between Objects** |
title_full | Fluorescence Microscopy Visualization of Contacts Between Objects** |
title_fullStr | Fluorescence Microscopy Visualization of Contacts Between Objects** |
title_full_unstemmed | Fluorescence Microscopy Visualization of Contacts Between Objects** |
title_short | Fluorescence Microscopy Visualization of Contacts Between Objects** |
title_sort | fluorescence microscopy visualization of contacts between objects** |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471612/ https://www.ncbi.nlm.nih.gov/pubmed/25631338 http://dx.doi.org/10.1002/anie.201410240 |
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