A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation

The utility of stem cells and their progeny in adult transplantation models has been limited by poor survival and integration. We designed an injectable and bioresorbable hydrogel blend of hyaluronan and methylcellulose (HAMC) and tested it with two cell types in two animal models, thereby gaining a...

Descripción completa

Detalles Bibliográficos
Autores principales: Ballios, Brian G., Cooke, Michael J., Donaldson, Laura, Coles, Brenda L.K., Morshead, Cindi M., van der Kooy, Derek, Shoichet, Molly S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471829/
https://www.ncbi.nlm.nih.gov/pubmed/25981414
http://dx.doi.org/10.1016/j.stemcr.2015.04.008
_version_ 1782376970042998784
author Ballios, Brian G.
Cooke, Michael J.
Donaldson, Laura
Coles, Brenda L.K.
Morshead, Cindi M.
van der Kooy, Derek
Shoichet, Molly S.
author_facet Ballios, Brian G.
Cooke, Michael J.
Donaldson, Laura
Coles, Brenda L.K.
Morshead, Cindi M.
van der Kooy, Derek
Shoichet, Molly S.
author_sort Ballios, Brian G.
collection PubMed
description The utility of stem cells and their progeny in adult transplantation models has been limited by poor survival and integration. We designed an injectable and bioresorbable hydrogel blend of hyaluronan and methylcellulose (HAMC) and tested it with two cell types in two animal models, thereby gaining an understanding of its general applicability for enhanced cell distribution, survival, integration, and functional repair relative to conventional cell delivery in saline. HAMC improves cell survival and integration of retinal stem cell (RSC)-derived rods in the retina. The pro-survival mechanism of HAMC is ascribed to the interaction of the CD44 receptor with HA. Transient disruption of the retinal outer limiting membrane, combined with HAMC delivery, results in significantly improved rod survival and visual function. HAMC also improves the distribution, viability, and functional repair of neural stem and progenitor cells (NSCs). The HAMC delivery system improves cell transplantation efficacy in two CNS models, suggesting broad applicability.
format Online
Article
Text
id pubmed-4471829
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-44718292015-06-22 A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation Ballios, Brian G. Cooke, Michael J. Donaldson, Laura Coles, Brenda L.K. Morshead, Cindi M. van der Kooy, Derek Shoichet, Molly S. Stem Cell Reports Article The utility of stem cells and their progeny in adult transplantation models has been limited by poor survival and integration. We designed an injectable and bioresorbable hydrogel blend of hyaluronan and methylcellulose (HAMC) and tested it with two cell types in two animal models, thereby gaining an understanding of its general applicability for enhanced cell distribution, survival, integration, and functional repair relative to conventional cell delivery in saline. HAMC improves cell survival and integration of retinal stem cell (RSC)-derived rods in the retina. The pro-survival mechanism of HAMC is ascribed to the interaction of the CD44 receptor with HA. Transient disruption of the retinal outer limiting membrane, combined with HAMC delivery, results in significantly improved rod survival and visual function. HAMC also improves the distribution, viability, and functional repair of neural stem and progenitor cells (NSCs). The HAMC delivery system improves cell transplantation efficacy in two CNS models, suggesting broad applicability. Elsevier 2015-05-14 /pmc/articles/PMC4471829/ /pubmed/25981414 http://dx.doi.org/10.1016/j.stemcr.2015.04.008 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Ballios, Brian G.
Cooke, Michael J.
Donaldson, Laura
Coles, Brenda L.K.
Morshead, Cindi M.
van der Kooy, Derek
Shoichet, Molly S.
A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation
title A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation
title_full A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation
title_fullStr A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation
title_full_unstemmed A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation
title_short A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation
title_sort hyaluronan-based injectable hydrogel improves the survival and integration of stem cell progeny following transplantation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471829/
https://www.ncbi.nlm.nih.gov/pubmed/25981414
http://dx.doi.org/10.1016/j.stemcr.2015.04.008
work_keys_str_mv AT balliosbriang ahyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT cookemichaelj ahyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT donaldsonlaura ahyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT colesbrendalk ahyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT morsheadcindim ahyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT vanderkooyderek ahyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT shoichetmollys ahyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT balliosbriang hyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT cookemichaelj hyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT donaldsonlaura hyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT colesbrendalk hyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT morsheadcindim hyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT vanderkooyderek hyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation
AT shoichetmollys hyaluronanbasedinjectablehydrogelimprovesthesurvivalandintegrationofstemcellprogenyfollowingtransplantation

Ejemplares similares