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Prohibitin as a novel autoantigen in rheumatoid arthritis
THE AIM OF THIS STUDY: The aim of this study was to verify whether prohibitin is a novel autoantigen in rheumatoid arthritis. MATERIAL AND METHODS: First, recombinant human prohibitin (rhPHB) protein was cloned, expressed, and purified. Then the anti-prohibitin autoantibodies were detected by wester...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Polish Society of Experimental and Clinical Immunology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4472543/ https://www.ncbi.nlm.nih.gov/pubmed/26155187 http://dx.doi.org/10.5114/ceji.2015.50837 |
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author | Shi, Li-Li Chen, Peng Xun, Yi-Ping Yang, Wei-Kang Yang, Chun-He Chen, Guang-Yu Du, Hong-Wu |
author_facet | Shi, Li-Li Chen, Peng Xun, Yi-Ping Yang, Wei-Kang Yang, Chun-He Chen, Guang-Yu Du, Hong-Wu |
author_sort | Shi, Li-Li |
collection | PubMed |
description | THE AIM OF THIS STUDY: The aim of this study was to verify whether prohibitin is a novel autoantigen in rheumatoid arthritis. MATERIAL AND METHODS: First, recombinant human prohibitin (rhPHB) protein was cloned, expressed, and purified. Then the anti-prohibitin autoantibodies were detected by western blotting by using rhPHB protein to incubate sera from patients with rheumatoid arthritis (RA). Next, immunoprecipitation was employed to further illustrate whether anti-prohibitin antibodies exist in RA patients. And finally, autoantibodies against the rhPHB protein were investigated using a homemade ELISA kit through the assessment of 258 real clinical samples. RESULTS: It was revealed that anti-prohibitin antibodies existed in the sera of patients with RA. Reactivity of serum IgG against rhPHB was detected in 26 of 86 RA patients (30.3%), 7 of 86 systemic lupus erythematosus (SLE) patients (8.1%), and 1 of 86 apparently healthy donors (HC) (1.2%). CONCLUSIONS: Prohibitin was proved to be a novel autoantigen and the corresponding anti-prohibitin autoantibodies were present in the RA patients' blood circulation. |
format | Online Article Text |
id | pubmed-4472543 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Polish Society of Experimental and Clinical Immunology |
record_format | MEDLINE/PubMed |
spelling | pubmed-44725432015-07-07 Prohibitin as a novel autoantigen in rheumatoid arthritis Shi, Li-Li Chen, Peng Xun, Yi-Ping Yang, Wei-Kang Yang, Chun-He Chen, Guang-Yu Du, Hong-Wu Cent Eur J Immunol Original Article THE AIM OF THIS STUDY: The aim of this study was to verify whether prohibitin is a novel autoantigen in rheumatoid arthritis. MATERIAL AND METHODS: First, recombinant human prohibitin (rhPHB) protein was cloned, expressed, and purified. Then the anti-prohibitin autoantibodies were detected by western blotting by using rhPHB protein to incubate sera from patients with rheumatoid arthritis (RA). Next, immunoprecipitation was employed to further illustrate whether anti-prohibitin antibodies exist in RA patients. And finally, autoantibodies against the rhPHB protein were investigated using a homemade ELISA kit through the assessment of 258 real clinical samples. RESULTS: It was revealed that anti-prohibitin antibodies existed in the sera of patients with RA. Reactivity of serum IgG against rhPHB was detected in 26 of 86 RA patients (30.3%), 7 of 86 systemic lupus erythematosus (SLE) patients (8.1%), and 1 of 86 apparently healthy donors (HC) (1.2%). CONCLUSIONS: Prohibitin was proved to be a novel autoantigen and the corresponding anti-prohibitin autoantibodies were present in the RA patients' blood circulation. Polish Society of Experimental and Clinical Immunology 2015-04-22 2015 /pmc/articles/PMC4472543/ /pubmed/26155187 http://dx.doi.org/10.5114/ceji.2015.50837 Text en Copyright © Central European Journal of Immunology 2015 http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Shi, Li-Li Chen, Peng Xun, Yi-Ping Yang, Wei-Kang Yang, Chun-He Chen, Guang-Yu Du, Hong-Wu Prohibitin as a novel autoantigen in rheumatoid arthritis |
title | Prohibitin as a novel autoantigen in rheumatoid arthritis |
title_full | Prohibitin as a novel autoantigen in rheumatoid arthritis |
title_fullStr | Prohibitin as a novel autoantigen in rheumatoid arthritis |
title_full_unstemmed | Prohibitin as a novel autoantigen in rheumatoid arthritis |
title_short | Prohibitin as a novel autoantigen in rheumatoid arthritis |
title_sort | prohibitin as a novel autoantigen in rheumatoid arthritis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4472543/ https://www.ncbi.nlm.nih.gov/pubmed/26155187 http://dx.doi.org/10.5114/ceji.2015.50837 |
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