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Moderate Exercise Mitigates the Detrimental Effects of Aging on Tendon Stem Cells

Aging is known to cause tendon degeneration whereas moderate exercise imparts beneficial effects on tendons. Since stem cells play a vital role in maintaining tissue integrity, in this study we aimed to define the effects of aging and moderate exercise on tendon stem/progenitor cells (TSCs) using in...

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Detalles Bibliográficos
Autores principales: Zhang, Jianying, Wang, James H-C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4472753/
https://www.ncbi.nlm.nih.gov/pubmed/26086850
http://dx.doi.org/10.1371/journal.pone.0130454
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author Zhang, Jianying
Wang, James H-C.
author_facet Zhang, Jianying
Wang, James H-C.
author_sort Zhang, Jianying
collection PubMed
description Aging is known to cause tendon degeneration whereas moderate exercise imparts beneficial effects on tendons. Since stem cells play a vital role in maintaining tissue integrity, in this study we aimed to define the effects of aging and moderate exercise on tendon stem/progenitor cells (TSCs) using in vitro and in vivo models. TSCs derived from aging mice (9 and 24 months) proliferated significantly slower than TSCs obtained from young mice (2.5 and 5 months). In addition, expression of the stem cell markers Oct-4, nucleostemin (NS), Sca-1 and SSEA-1 in TSCs decreased in an age-dependent manner. Interestingly, moderate mechanical stretching (4%) of aging TSCs in vitro significantly increased the expression of the stem cell marker, NS, but 8% stretching decreased NS expression. Similarly, 4% mechanical stretching increased the expression of Nanog, another stem cell marker, and the tenocyte-related genes, collagen I and tenomodulin. However, 8% stretching increased expression of the non-tenocyte-related genes, LPL, Sox-9 and Runx-2, while 4% stretching had minimal effects on the expression of these genes. In the in vivo study, moderate treadmill running (MTR) of aging mice (9 months) resulted in the increased proliferation rate of aging TSCs in culture, decreased lipid deposition, proteoglycan accumulation and calcification, and increased the expression of NS in the patellar tendons. These findings indicate that while aging impairs the proliferative ability of TSCs and reduces their stemness, moderate exercise can mitigate the deleterious effects of aging on TSCs and therefore may be responsible for decreased aging-induced tendon degeneration.
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spelling pubmed-44727532015-06-29 Moderate Exercise Mitigates the Detrimental Effects of Aging on Tendon Stem Cells Zhang, Jianying Wang, James H-C. PLoS One Research Article Aging is known to cause tendon degeneration whereas moderate exercise imparts beneficial effects on tendons. Since stem cells play a vital role in maintaining tissue integrity, in this study we aimed to define the effects of aging and moderate exercise on tendon stem/progenitor cells (TSCs) using in vitro and in vivo models. TSCs derived from aging mice (9 and 24 months) proliferated significantly slower than TSCs obtained from young mice (2.5 and 5 months). In addition, expression of the stem cell markers Oct-4, nucleostemin (NS), Sca-1 and SSEA-1 in TSCs decreased in an age-dependent manner. Interestingly, moderate mechanical stretching (4%) of aging TSCs in vitro significantly increased the expression of the stem cell marker, NS, but 8% stretching decreased NS expression. Similarly, 4% mechanical stretching increased the expression of Nanog, another stem cell marker, and the tenocyte-related genes, collagen I and tenomodulin. However, 8% stretching increased expression of the non-tenocyte-related genes, LPL, Sox-9 and Runx-2, while 4% stretching had minimal effects on the expression of these genes. In the in vivo study, moderate treadmill running (MTR) of aging mice (9 months) resulted in the increased proliferation rate of aging TSCs in culture, decreased lipid deposition, proteoglycan accumulation and calcification, and increased the expression of NS in the patellar tendons. These findings indicate that while aging impairs the proliferative ability of TSCs and reduces their stemness, moderate exercise can mitigate the deleterious effects of aging on TSCs and therefore may be responsible for decreased aging-induced tendon degeneration. Public Library of Science 2015-06-18 /pmc/articles/PMC4472753/ /pubmed/26086850 http://dx.doi.org/10.1371/journal.pone.0130454 Text en © 2015 Zhang, Wang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Jianying
Wang, James H-C.
Moderate Exercise Mitigates the Detrimental Effects of Aging on Tendon Stem Cells
title Moderate Exercise Mitigates the Detrimental Effects of Aging on Tendon Stem Cells
title_full Moderate Exercise Mitigates the Detrimental Effects of Aging on Tendon Stem Cells
title_fullStr Moderate Exercise Mitigates the Detrimental Effects of Aging on Tendon Stem Cells
title_full_unstemmed Moderate Exercise Mitigates the Detrimental Effects of Aging on Tendon Stem Cells
title_short Moderate Exercise Mitigates the Detrimental Effects of Aging on Tendon Stem Cells
title_sort moderate exercise mitigates the detrimental effects of aging on tendon stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4472753/
https://www.ncbi.nlm.nih.gov/pubmed/26086850
http://dx.doi.org/10.1371/journal.pone.0130454
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