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Alternative preparation of inclusion bodies excludes interfering non-protein contaminants and improves the yield of recombinant proinsulin
The goal of simple, high-yield expression and purification of recombinant human proinsulin has proven to be a considerable challenge. First, proinsulin forms inclusion bodies during bacterial expression. While this phenomenon can be exploited as a capture step, conventionally prepared inclusion bodi...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4472945/ https://www.ncbi.nlm.nih.gov/pubmed/26150942 http://dx.doi.org/10.1016/j.mex.2014.07.005 |