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A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents

A liquid chromatography–MS (LC-MS) metabolomics analysis of plasma from portal–arterial catheterised pigs fed breads prepared with whole-grain rye or wheat flour with added concentrated arabinoxylan (AX) or β-glucan (BG) was conducted. Comparison of the effects of concentrated fibres with whole grai...

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Autores principales: Nielsen, Kirstine Lykke, Hedemann, Mette Skou, Lærke, Helle Nygaard, Jørgensen, Henry, Bach Knudsen, Knud Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4473157/
https://www.ncbi.nlm.nih.gov/pubmed/26101587
http://dx.doi.org/10.1017/jns.2014.14
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author Nielsen, Kirstine Lykke
Hedemann, Mette Skou
Lærke, Helle Nygaard
Jørgensen, Henry
Bach Knudsen, Knud Erik
author_facet Nielsen, Kirstine Lykke
Hedemann, Mette Skou
Lærke, Helle Nygaard
Jørgensen, Henry
Bach Knudsen, Knud Erik
author_sort Nielsen, Kirstine Lykke
collection PubMed
description A liquid chromatography–MS (LC-MS) metabolomics analysis of plasma from portal–arterial catheterised pigs fed breads prepared with whole-grain rye or wheat flour with added concentrated arabinoxylan (AX) or β-glucan (BG) was conducted. Comparison of the effects of concentrated fibres with whole grains has received little attention. Six female catheterised pigs were given two white wheat breads with wheat AX or oat BG, two rye breads with ground rye (GR) or intact rye kernels (RK), and a control white wheat bread (WF) on separate occasions in a randomised cross-over design. The amount of available carbohydrate was similar for the five breads but varied in the content of protein. Plasma was collected continuously for 4 h after feeding. Glucose levels in the portal vein were reduced postprandially in response to the AX, GR and RK breads that had high contents of AX compared with WF bread (P < 0·03). AX and RK breads further tended to decrease plasma levels of some lysophosphatidylcholine species (P ≤ 0·10). The abundance of amino acids in plasma correlated with the protein contents in the breads and leucine uptake significantly affected insulin secretion in the mesenteric artery. In conclusion, the present study revealed that concentrated AX in wheat bread had similar positive effects as whole-grain rye bread on glucose and lipid metabolism.
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spelling pubmed-44731572015-06-22 A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents Nielsen, Kirstine Lykke Hedemann, Mette Skou Lærke, Helle Nygaard Jørgensen, Henry Bach Knudsen, Knud Erik J Nutr Sci Molecular Nutrition A liquid chromatography–MS (LC-MS) metabolomics analysis of plasma from portal–arterial catheterised pigs fed breads prepared with whole-grain rye or wheat flour with added concentrated arabinoxylan (AX) or β-glucan (BG) was conducted. Comparison of the effects of concentrated fibres with whole grains has received little attention. Six female catheterised pigs were given two white wheat breads with wheat AX or oat BG, two rye breads with ground rye (GR) or intact rye kernels (RK), and a control white wheat bread (WF) on separate occasions in a randomised cross-over design. The amount of available carbohydrate was similar for the five breads but varied in the content of protein. Plasma was collected continuously for 4 h after feeding. Glucose levels in the portal vein were reduced postprandially in response to the AX, GR and RK breads that had high contents of AX compared with WF bread (P < 0·03). AX and RK breads further tended to decrease plasma levels of some lysophosphatidylcholine species (P ≤ 0·10). The abundance of amino acids in plasma correlated with the protein contents in the breads and leucine uptake significantly affected insulin secretion in the mesenteric artery. In conclusion, the present study revealed that concentrated AX in wheat bread had similar positive effects as whole-grain rye bread on glucose and lipid metabolism. Cambridge University Press 2014-08-22 /pmc/articles/PMC4473157/ /pubmed/26101587 http://dx.doi.org/10.1017/jns.2014.14 Text en © The Author(s) 2014 The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution license <http://creativecommons.org/licenses/by/3.0/.
spellingShingle Molecular Nutrition
Nielsen, Kirstine Lykke
Hedemann, Mette Skou
Lærke, Helle Nygaard
Jørgensen, Henry
Bach Knudsen, Knud Erik
A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents
title A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents
title_full A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents
title_fullStr A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents
title_full_unstemmed A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents
title_short A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents
title_sort metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents
topic Molecular Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4473157/
https://www.ncbi.nlm.nih.gov/pubmed/26101587
http://dx.doi.org/10.1017/jns.2014.14
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