Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

We determined that the Vitek MS Plus matrix-assisted laser desorption ionization–time of flight mass spectrometry using research-use-only (RUO) v.4.12 and in vitro-diagnostic (IVD) v.3.0 databases accurately identified 41 Mycobacterium abscessus subsp. abscessus and 13 M. abscessus subsp. massiliens...

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Detalles Bibliográficos
Autores principales: Panagea, Theofano, Pincus, David H., Grogono, Dorothy, Jones, Melissa, Bryant, Josephine, Parkhill, Julian, Floto, R. Andres, Gilligan, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2015
Materias:
Mycobacteriology and Aerobic Actinomycetes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4473197/
https://www.ncbi.nlm.nih.gov/pubmed/25948607
http://dx.doi.org/10.1128/JCM.00494-15
Descripción
Sumario:We determined that the Vitek MS Plus matrix-assisted laser desorption ionization–time of flight mass spectrometry using research-use-only (RUO) v.4.12 and in vitro-diagnostic (IVD) v.3.0 databases accurately identified 41 Mycobacterium abscessus subsp. abscessus and 13 M. abscessus subsp. massiliense isolates identified by whole-genome sequencing to the species but not the subspecies level, from Middlebrook 7H11 and Burkholderia cepacia selective agars. Peak analysis revealed three peaks potentially able to differentiate between subspecies.

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